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Mini-titins in striated and smooth molluscan muscles: structure, location and immunological crossreactivity

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Summary

Invertebrate mini-titins are members of a class of myosin-binding proteins belonging to the immunoglobulin superfamily that may have structural and/or regulatory properties. We have isolated mini-titins from three molluscan sources: the striated and smooth adductor muscles of the scallop, and the smooth catch muscles of the mussel. Electron microscopy reveals flexible rod-like molecules about 0.2 μm long and 30 Å wide with a distinctive polarity. Antibodies to scallop mini-titin label the A-band and especially the A/I junction of scallop striated muscle myofibrils by indirect immunofluorescence and immuno-electron microscopy. This antibody crossreacts with mini-titins in scallop smooth and Mytilus catch muscles, as well as with proteins in striated muscles from Limulus, Lethocerus (asynchronous flight muscle), and crayfish. It labels the A/I junction (I-region in Lethocerus) in these striated muscles as well as in chicken skeletal muscle. Antibodies to the repetitive immunoglobulin-like regions and also to the kinase domain of nematode twitchin crossreact with scallop mini-titin and label the A-band of scallop myofibrils. Electron microscopy of single molecules shows that antibodies to twitchin kinase bind to scallop mini-titin near one end of the molecule, suggesting how the scallop structure might be aligned with the sequence of nematode twitchin.

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Authors and Affiliations

  1. Rosenstiel Center, Brandeis University, 02254, Waltham, MA, USA

    Peter Vibert, Stacey M. Edelstein & Bruce W. Elliott Jr

  2. Department of Experimental Medicine and Biological Chemistry, Universita' Tor Vergata, Viale Marx, 15/43, Rome, Italy

    Loriana Castellani

  3. CNR, Institute of Neurobiology, Viale Marx, 15/43, Rome, Italy

    Loriana Castellani

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  1. Peter Vibert
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  2. Stacey M. Edelstein
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  3. Loriana Castellani
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  4. Bruce W. Elliott Jr
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Vibert, P., Edelstein, S.M., Castellani, L. et al. Mini-titins in striated and smooth molluscan muscles: structure, location and immunological crossreactivity. J Muscle Res Cell Motil 14, 598–607 (1993). https://doi.org/10.1007/BF00141557

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  • DOI: https://doi.org/10.1007/BF00141557

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