Skip to main content
SpringerLink
Log in

Synthesis of Polymeric Matrices for Adsorption and Purification of Endoglucanase

  • Original Paper
  • Published:
Journal of Polymers and the Environment Aims and scope Submit manuscript

Abstract

Chitosan (CHS), chitosan–Eudragit® EPO (CHS–EPO) and chitosan beads partially cross-linked with glutaraldehyde (CHS–GLUT) were obtained in order to improve the adsorption selectivity of endoglucanase from a fungal culture obtained under SSF by Aspergillus niger using soybean harvest residues as support. The matrices synthetized were characterized in terms of physical and chemical changes. Fourier-transformed infrared spectroscopy with attenuated total reflectance device (FTIR-ATR) was employed to verify the chemical changes on the CHS matrix after the synthesis of CHS–GLUT and CHS–EPO. Scanning electron microscopy (SEM) was performed to compare the surface morphology of the polymeric beads. Two variables, purification factor and yield percentage of the adsorption process, were analyzed using a bifactorial ANOVA considering the matrix–time first order interaction. SEM results exhibited greater surface roughness in the CHS–GLUT and CHS–EPO matrices which may enhance endoglucanase adsorption. FTIR-ATR results confirmed an effective chemical modification of the CHS matrix after crosslinking with GLUT and corroborated the efficiency of the synthesis of the CHS–EPO matrix by the presence of chemical groups of the EPO polymer. An endoglucanase purification factor close to 9 was achieved with the CHS–GLUT matrix and a yield percentage of 60% was obtained with the CHS–EPO matrix. Bifactorial ANOVA results showed the matrix–time interaction to be significant for both variables. The CHS–GLUT matrix with low crosslinking times and the novel CHS–EPO matrix could be included in the bioseparation stage of endoglucanase using a simple and a low-cost method such as batch adsorption.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Saqib AA, Hassan M, Khan NF, Baig S (2010) Thermostability of crude endoglucanase from Aspergillus fumigatus grown under solid state fermentation (SSF) and submerged fermentation (SmF). Process Biochem 45:641–646

    Article  CAS  Google Scholar 

  2. Bassani G, Farruggia B, Picó G (2011) Cationic polyelectrolytes–lipases complexes formation as tool for recovery of these enzymes from their natural sources. Int J Biol Macromol 49:351–355

    Article  CAS  Google Scholar 

  3. Valetti NW, Boeris V, Picó G (2013) Characterization of chymotrypsin–ι-carrageenan complex in aqueous solution: a solubility and thermodynamical stability study. Int J Biol Macromol 52:45–51

    Article  CAS  Google Scholar 

  4. Yin L-J, Lin H-H, Xiao Z-R (2010) Purification and characterization of a cellulase from Bacillus subtilis YJ1. J Mar Sci Technol 18:466–471

    Google Scholar 

  5. Gupta V, Prasanna R, Chaudhary V, Nain L (2012) Biochemical, structural and functional characterization of two novel antifungal endoglucanases from Anabaena laxa. Biocatal Agric Biotechnol 1:338–347

    CAS  Google Scholar 

  6. Murashima K, Nishimura T, Nakamura Y, Koga J, Moriya T, Sumida N, Yaguchi T, Kono T (2002) Purification and characterization of new endo-1, 4-β-d-glucanases from Rhizopus oryzae. Enzyme Microb Tech 30:319–326

    Article  CAS  Google Scholar 

  7. Daoud FB-O, Kaddour S, Sadoun T (2010) Adsorption of cellulase Aspergillus niger on a commercial activated carbon: kinetics and equilibrium studies. Coll Surf B 75:93–99

    Article  CAS  Google Scholar 

  8. Dorraji MS, Amani-Ghadim A, Hanifehpour Y, Joo SW, Figoli A, Carraro M, Tasselli F (2017) Performance of chitosan based nanocomposite hollow fibers in the removal of selenium (IV) from water. Chem Eng Res Des 117:309–317

    Article  Google Scholar 

  9. Bibi F, Irshad M, Anwar Z, Bhatti KH, Raza A (2017) Improved catalytic functionalities of purified pristine and chitosan-immobilized polygalacturonase, and pectin lyase. Chem Eng Res Des 128:146–154

    Article  CAS  Google Scholar 

  10. Dayarian S, Zamani A, Moheb A, Masoomi M (2014) Physico-mechanical properties of films of chitosan, carboxymethyl chitosan, and their blends. J Polym Environ 22:409–416

    Article  CAS  Google Scholar 

  11. Sila A, Mlaik N, Sayari N, Balti R, Bougatef A (2014) Chitin and chitosan extracted from shrimp waste using fish proteases aided process: efficiency of chitosan in the treatment of unhairing effluents. J Polym Environ 22:78–87

    Article  CAS  Google Scholar 

  12. Islam S, Bhuiyan MR, Islam M (2017) Chitin and chitosan: structure, properties and applications in biomedical engineering. J Polym Environ 25:854–866

    Article  CAS  Google Scholar 

  13. Asgher M, Noreen S, Bilal M (2017) Enhancing catalytic functionality of Trametes versicolor IBL-04 laccase by immobilization on chitosan microspheres. Chem Eng Res Des 119:1–11

    Article  CAS  Google Scholar 

  14. Ma H-F, Meng G, Cui B-K, Si J, Dai Y-C (2018) Chitosan crosslinked with genipin as supporting matrix for biodegradation of synthetic dyes: laccase immobilization and characterization. Chem Eng Res Des 132:664–676

    Article  CAS  Google Scholar 

  15. Vieira RS, Beppu MM (2006) Dynamic and static adsorption and desorption of Hg(II) ions on chitosan membranes and spheres. Water Res 40:1726–1734

    Article  CAS  Google Scholar 

  16. Moustafine RI, Margulis EB, Sibgatullina LF, Kemenova VA, Van den Mooter G (2008) Comparative evaluation of interpolyelectrolyte complexes of chitosan with Eudragit® L100 and Eudragit® L100-55 as potential carriers for oral controlled drug delivery. Eur J Pharm Biopharm 70:215–225

    Article  CAS  Google Scholar 

  17. Kamari A, Ngah WW, Chong M, Cheah M (2009) Sorption of acid dyes onto GLA and H2SO4 cross-linked chitosan beads. Desalination 249:1180–1189

    Article  CAS  Google Scholar 

  18. Ngah WW, Endud C, Mayanar R (2002) Removal of copper (II) ions from aqueous solution onto chitosan and cross-linked chitosan beads. React Funct Polym 50:181–190

    Article  CAS  Google Scholar 

  19. Ngah WW, Ab Ghani S, Kamari A (2005) Adsorption behaviour of Fe(II) and Fe(III) ions in aqueous solution on chitosan and cross-linked chitosan beads. Bioresour Technol 96:443–450

    Article  Google Scholar 

  20. Beppu MM, Arruda EJ, Santana CC (1999) Síntese e caracterização de estruturas densas e porosas de quitosana. Polímeros 9(4):163–169

    Article  CAS  Google Scholar 

  21. Zhang Y, Xu J, Yuan Z, Xu H, Yu Q (2010) Artificial neural network-genetic algorithm based optimization for the immobilization of cellulase on the smart polymer Eudragit L-100. Bioresour Technol 101:3153–3158

    Article  CAS  Google Scholar 

  22. Zhang Y, Xu J-L, Li D, Yuan Z-H (2010) Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100. Biocatal Biotransform 28:313–319

    Article  CAS  Google Scholar 

  23. Homaei AA, Sariri R, Vianello F, Stevanato R (2013) Enzyme immobilization: an update. J Chem Biol 6:185–205

    Article  Google Scholar 

  24. Warburg O, Christian W (1941) Isolation and crystallization of the glycolytic enzyme enolase. Biochem Zeitschrift 31:384–421

    Google Scholar 

  25. Lenth RV,Hervé M, (2015) lsmeans: Least-squares means. R package version 2.17: 20-3

  26. Team RC (2015) R: A language and environment for statistical computing.

  27. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680

    Article  CAS  Google Scholar 

  28. Medve J, Lee D, Tjerneld F (1998) Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography. J Chromatogr A 808:153–165

    Article  CAS  Google Scholar 

  29. Rodrigues EC, Bezerra BT, Farias BV, Adriano WS, Vieira RS, Azevedo DC, Silva IJ Jr (2013) Adsorption of cellulase isolated from Aspergillus niger on chitosan/alginate particles functionalized with epichlorohydrin. Adsorpt Sci Technol 31:17–34

    Article  CAS  Google Scholar 

  30. Corazzari I, Nisticò R, Turci F, Faga MG, Franzoso F, Tabasso S, Magnacca G (2015) Advanced physico-chemical characterization of chitosan by means of TGA coupled on-line with FTIR and GCMS: Thermal degradation and water adsorption capacity. Polym Degrad Stab 112:1–9

    Article  CAS  Google Scholar 

  31. Pawlak A, Mucha M (2003) Thermogravimetric and FTIR studies of chitosan blends. Thermochim Acta 396:153–166

    Article  CAS  Google Scholar 

  32. Beppu M, Vieira R, Aimoli C, Santana C (2007) Crosslinking of chitosan membranes using glutaraldehyde: effect on ion permeability and water absorption. J Membr Sci 301:126–130

    Article  CAS  Google Scholar 

  33. Jeganathan B, Prakya V (2015) Interpolyelectrolyte complexes of Eudragit® EPO with hypromellose acetate succinate and Eudragit® EPO with hypromellose phthalate as potential carriers for oral controlled drug delivery. AAPS Pharm Sci Tech 16:878–888

    Article  CAS  Google Scholar 

  34. Chandrasekaran N, Dimartino S, Fee CJ (2013) Study of the adsorption of proteins on stainless steel surfaces using QCM-D. Chem Eng Res Des 91:1674–1683

    Article  CAS  Google Scholar 

  35. Beppu M, Arruda E, Vieira R, Santos N (2004) Adsorption of Cu(II) on porous chitosan membranes functionalized with histidine. J Membr Sci 240:227–235

    Article  CAS  Google Scholar 

  36. Çetinus ŞA, Şahin E, Saraydin D (2009) Preparation of Cu(II) adsorbed chitosan beads for catalase immobilization. Food Chem 114:962–969

    Article  Google Scholar 

  37. Boggione MJ, Mahl CR, Beppu MM, Farruggia B (2017) Synthesis and characterization of chitosan membranes functionalized with amino acids and copper for adsorption of endoglucanase. Powder Technol 315:250–257

    Article  CAS  Google Scholar 

  38. Ferchak J, Pye E (1980) Saccharification of cellulose by the cellulolytic enzyme system of Thermomonospora species. I. Stability of cellulolytic activities with respect to time, temperature and pH. Biotech Bioeng 22:1515–1526

    Article  Google Scholar 

  39. Hussain Z, Gohar UF, Dilshad M, Khan MA (2014) Characterization of immobilized endo-1, 4-β-glucanase from Thermothoga petrophila takahata et al. Int J Biol Biotechnol 11:525–535

    CAS  Google Scholar 

  40. Trimukhe K, Mahadik N, Gokhale D, Varma A (2008) Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of Aspergillus niger. Int J Biol Macromol 43:422–425

    Article  CAS  Google Scholar 

  41. Khademi S, Zhang D, Swanson SM, Wartenberg A, Witte K, Meyer EF (2002) Determination of the structure of an endoglucanase from Aspergillus niger and its mode of inhibition by palladium chloride. Acta Crystallogr D 58:660–667

    Article  Google Scholar 

  42. Bakare M, Adewale I, Ajayi A, Shonukan O (2005) Purification and characterization of cellulase from the wild-type and two improved mutants of Pseudomonas fluorescens. Afr J Biotechnol 4:898–904

    CAS  Google Scholar 

  43. Bischoff KM, Rooney AP, Li X-L, Liu S, Hughes SR (2006) Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis. Biotechnol Lett 28:1761–1765

    Article  CAS  Google Scholar 

  44. Boggione MJ, Becher R, Farruggia B (2016) Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation. Biocatal Agric Biotechnol 7:118–126

    Google Scholar 

  45. Zhou J, Wang Y-H, Chu J, Zhuang Y-P, Zhang S-L, Yin P (2008) Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T 100-14. Bioresour Technol 99:6826–6833

    Article  CAS  Google Scholar 

  46. Adeoye A, Lateef A, Gueguim-Kana E (2015) Optimization of citric acid production using a mutant strain of Aspergillus niger on cassava peel substrate. Biocatal Agric Biotechnol 4:568–574

    Google Scholar 

  47. Boggione MJ, Allasia MB, Bassani G, Farruggia B (2016) Potential use of soybean hulls and waste paper as supports in SSF for cellulase production by Aspergillus niger. Biocatal Agric Biotechnol 6:1–8

    Google Scholar 

Download references

Acknowledgements

This work was supported by CONICET (PIP 112 2011 01 00578). Authors would like to thank the Staff from the English Department (Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario), for correcting the language of this manuscript.

Author information

Authors and Affiliations

  1. IPROByQ-CONICET, Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario, Suipacha 531, 2000, Rosario, Argentina

    María Julia Boggione, María Paula Zilli & Beatriz Farruggia

  2. Area of Statistics and Data Processing, Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario, Suipacha 531, 2000, Rosario, Argentina

    María Belén Allasia

Authors
  1. María Julia Boggione
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. María Paula Zilli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. María Belén Allasia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Beatriz Farruggia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to María Julia Boggione.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Boggione, M.J., Zilli, M.P., Allasia, M.B. et al. Synthesis of Polymeric Matrices for Adsorption and Purification of Endoglucanase. J Polym Environ 26, 4321–4330 (2018). https://doi.org/10.1007/s10924-018-1303-7

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10924-018-1303-7

Keywords

Search

Navigation