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Grapefruit juice exerts anti-osteoporotic activities in a prednisolone-induced osteoporosis rat femoral fracture model, possibly via the RANKL/OPG axis

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Abstract

This study aimed to shed light on the protective and therapeutic anti-osteoporotic effects and mechanisms of action of grapefruit juice (GFJ) on prednisolone-induced osteoporosis a rat femoral fracture model. We found that treating rats with GFJ before and/or after prednisolone-induced osteoporosis resulted in increased bone density, total mineral content, and calcium content to counteract the osteoporotic effects of prednisolone. In parallel, the histological and ultrastructural results of the GFJ-treated groups correlated well with enhanced breaking strength of femurs subjected to a constant load. Furthermore, GFJ treatment before and after prednisolone-induced osteoporosis decreased plasma alkaline phosphatase and tartrate-resistant acid phosphatase activities and increased the level of insulin-like growth factor 1. Mechanistically, our immunohistochemistry study showed that GFJ ameliorated prednisolone-induced osteocalcin depletion, decreased receptor activator of nuclear factor kappa-B ligand (RANKL) expression, and increased osteoprotegerin (OPG) expression. GFJ showed a beneficial anti-osteoporotic effect against prednisolone-induced osteoporosis in rats, possibly via the RANKL/OPG axis, suggesting that GFJ might be a good candidate for developing anti-osteoporotic drugs.

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Authors and Affiliations

  1. Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan

    Eslam Muhammad Bastawy

  2. Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

    Eslam Muhammad Bastawy, Fatma Kamal Abd El-Hady & Monir Ali El-Ganzuri

  3. Zoology Department, Faculty of Science, Beni-Seuif University, Beni Suef, Egypt

    Rasha Rashad Ahmed

  4. Pharmacology and Experimental Oncology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt

    Amer Ali Abd El-Hafeez

  5. Department of Cellular and Molecular Medicine, School of medicine, University of California, San Diego, La Jolla, CA, USA

    Amer Ali Abd El-Hafeez

  6. Pharmacotherapy Department, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan

    Toru Hosoi & Koichiro Ozawa

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  1. Eslam Muhammad Bastawy
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Correspondence to Eslam Muhammad Bastawy or Amer Ali Abd El-Hafeez.

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The authors declare that there are no studies conducted with human participants. Animals were treated in accordance with the guidelines of the Committee on Animal Experimentation of Beni-Seuif University, Egypt.

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Bastawy, E.M., Ahmed, R.R., Abd El-Hafeez, A.A. et al. Grapefruit juice exerts anti-osteoporotic activities in a prednisolone-induced osteoporosis rat femoral fracture model, possibly via the RANKL/OPG axis. Cytotechnology 71, 769–783 (2019). https://doi.org/10.1007/s10616-019-00321-6

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