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Immunocytochemistry assay in BHK-21 cell line infected with Porcine Sapelovirus

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Abstract

The present study describes an immunocytochemistry (ICC) assay with self-raised hyperimmune sera and a Baby Hamster Kidney-21 (BHK-21) cell line infected with Porcine Sapelovirus (PSV). Sapelovivus/IVRI/SPF-c-6/2015 strain Indian PSV was isolated from the porcine IBRS-2 cell line and investigated for growth on non-porcine cell lines. After two passages, PSV was successfully grown in BHK-21 and produced the same cytopathic effects as in IBRS-2 such as shrinking of cytoplasm, rounding of cells and detachment of cells from the surface of flask within 24 h. For raising of hyperimmune sera, PSV was grown in IBRS-2 cell line up to the required volume and purified by ultracentrifugation. With self-raised hyperimmune sera in laboratory rats, ICC was performed in BHK-21 cells infected with PSV. Positive signals consisted of large granular aggregates of virus in the cytoplasm near the nucleus, suggesting that PSV can infect cell lines other than those of porcine origin.

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Acknowledgements

The authors are highly thankful to the institute’s authority for providing necessary facilities to carry out the research work. Funding was provided by ICAR-Indian Veterinary Research Institute.

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Authors and Affiliations

  1. Division of Pathology, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

    Swati Kumari, Rahul Singh, P. A. Desingu, P. K. Ray & G. Saikumar

  2. Physiology and Climatology Division, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

    G. Taru Sharma

  3. Molecular Pathology Laboratory, Swine Disease Laboratory, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

    G. Saikumar

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  1. Swati Kumari
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  2. Rahul Singh
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  3. P. A. Desingu
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  4. P. K. Ray
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  5. G. Taru Sharma
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Correspondence to G. Saikumar.

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All the experimental procedures on animals were carried out according to the recommendations and approval of the Institute Animal Ethics Committee (IAEC) as per the guidelines set forth by the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA).

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Kumari, S., Singh, R., Desingu, P.A. et al. Immunocytochemistry assay in BHK-21 cell line infected with Porcine Sapelovirus. Cytotechnology 71, 751–755 (2019). https://doi.org/10.1007/s10616-019-00315-4

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  • DOI: https://doi.org/10.1007/s10616-019-00315-4

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