Summary
Porcine enteroviruses isolated in the United Kingdom between 1972 and 1976 were compared with the 8 serotypes previously described and with human coxsackie B virus types 1 to 6 for ability to grow in different cell lines. This allowed the classification of all strains into 3 broad groups according to type of cytopathic effect in IBRS-2 cells and further subdivision on the basis of production of cytopathic effect in BHK 21, HeLa and VERO cells.
None of the porcine enterovirus strains was neutralized by antisera to human enteroviruses (Lim Benyesh-Melnick Pools) with the exception of swine vesicular disease virus, which was neutralised by coxsackie B5 antiserum. Antisera prepared either in gnotobiotic pigs or in guinea pigs against the 8 porcine enterovirus serotypes failed to neutralize 9 isolates which could be classified into 3 new serotypes, for which Nos. 9, 10 and 11 are proposed. Guinea pig sera could be used as an alternative to gnotobiotic pig sera for type differentiation.
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References
Alexander, T. J. L., Betts, A. O.: Further studies on porcine enteroviruses isolated at Cambridge. II. Serological grouping. Res. vet. Sci.8, 330–337 (1967).
Betts, A. O., Kelly, D. F., Lamont, P. H., Sheffy, B. E.: The isolation and characterisation of some enteroviruses from pigs. Vet. Rec.73, 752–755 (1961).
Brown, F., Cartwright, B.: Purification of radioactive foot-and-mouth disease virus. Nature (London)199, 1168–1170 (1963).
Brown, F., Crick, J.: Application of agar-gel diffusion analysis to a study of the antigenic structure of inactivated vaccines prepared from the virus of foot-and-mouth disease. J. Immunol.82, 444–447 (1959).
Brown, F., Goodridge, D., Burrows, R.: Infection of man by swine vesicular disease virus. J. comp. Pathol.86, 409–414 (1976).
Brown, F., Talbot, P., Burrows, R.: Antigenic differences between isolates of swine vesicular disease virus and their relationship to coxsackie B5 virus. Nature (London)245, 315–316 (1973).
Brown, F., Wild, F.: Variation in the coxsackievirus type B5 and its possible role in the etiology of swine vesicular disease. Intervirology3, 125–128 (1974).
Brown, F., Wild, T. F., Rowe, L. W., Underwood, B. O., Harris, T. J. R.: Comparison of swine vesicular disease virus and coxsackie B5 virus by serological and RNA hybridization methods. J. gen. Virol.31, 231–237 (1976).
Buckley, L. S., Osborne, R. W., Pereira, H. G.: Laboratory diagnosis of foot-and-mouth disease and swine vesicular disease. Bull. Off. Int. Epiz.83, 123–129 (1975).
Christofinis, G. J., Edington, N., Betts, A. O., Prydie, J., Pirie, G.: Preparation in gnotobiotic animals of monospecific reference serums against viruses. Amer. J. vet. Res.33, 1915–1923 (1972).
Dawe, P. S., Forman, A. J., Smale, C. J.: A preliminary investigation of the swine vesicular disease epidemic in Britain. Nature (London)241, 540–542 (1973).
Dunne, H. W.: Porcine Enteroviruses. In:Dunne, H. W., Leman, A. D. (eds.), Diseases of swine, 4th ed., chap. 15. Ames, Iowa: Iowa State University Press 1975.
Dunne, H. W., Kradel, D. C., Clark, C. D., Bubash, G. R., Ammerman, E.: Porcine enteroviruses: A serologic comparison of thirty-eight Pennsylvania isolates with other reported North American strains, Teschen, Talfan and T80 serums—a progress report. Amer. J. vet. Res.28, 557–567 (1967).
Dunne, H. W., Wang, J. T., Ammerman, E. H.: Classification of North American porcine enteroviruses: A comparison with European and Japanese strains. Infec. Immun.4, 619–631 (1971).
Graves, J. H.: Serological relationship of swine vesicular disease virus and coxsackie B5 virus. Nature (London)245, 314–315 (1973).
Harris, T. J. R., Brown, F.: Correlation of polypeptide composition with antigenic variation in the swine vesicular disease and coxsackie B5 viruses. Nature (London)258, 758–760 (1975).
Harris, T. J. R., Brown, F.: Swine vesicular disease and its relationship to coxsackie B5 virus. ARC Research Review3, 18–21 (1977).
Harris, T. J. R., Doel, T. R., Brown, F.: Molecular aspects of the antigenic variation of swine vesicular disease and coxsackie B5 viruses. J. gen. Virol.35, 299–315 (1977).
Kapikian, A. Z., and 17 others: Rhinoviruses: A numbering system. Nature (London)213, 761–763 (1967).
Karber, G.: Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch. exp. Pathol. Pharmakol.162, 480–483 (1931).
Schmidt, N. J., Melnick, J. L., Wenner, H. A., Ho, H. H., Burkhardt, M. A.: Evaluation of enterovirus immune horse serum pools for identification of virus field strains. Bull. Wld. Hlth. Org.45, 317–330 (1971).
Young, N. A.: Polioviruses, coxsackieviruses and echoviruses: Comparison of the genomes by RNA hybridization. J. Virol.11, 832–839 (1973).
Zoletto, R.: Differential characteristics of swine enteroviruses. Vet. Ital.16, 3–12 (translation16, 13–20) (1965).
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Knowles, N.J., Buckley, L.S. & Pereira, H.G. Classification of porcine enteroviruses by antigenic analysis and cytopathic effects in tissue culture: Description of 3 new serotypes. Archives of Virology 62, 201–208 (1979). https://doi.org/10.1007/BF01317552
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DOI: https://doi.org/10.1007/BF01317552