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Cytotechnology

, Volume 68, Issue 5, pp 2061–2073 | Cite as

Generation and characterization of human cardiac resident and non-resident mesenchymal stem cell

  • Baskar Subramani
  • Sellamuthu Subbannagounder
  • Sekar Palanivel
  • Chithra Ramanathanpullai
  • Sivakumar Sivalingam
  • Azhari Yakub
  • Manjunath SadanandaRao
  • Arivudainambi Seenichamy
  • Ashok Kumar Pandurangan
  • Jun Jie Tan
  • Rajesh RamasamyEmail author
Original Article

Abstract

Despite the surgical and other insertional interventions, the complete recuperation of myocardial disorders is still elusive due to the insufficiency of functioning myocardiocytes. Thus, the use of stem cells to regenerate the affected region of heart becomes a prime important. In line with this human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have gained considerable interest due to their potential use for mesodermal cell based replacement therapy and tissue engineering. Since MSCs are harvested from various organs and anatomical locations of same organism, thus the cardiac regenerative potential of human cardiac-derived MSCs (hC-MSCs) and human umbilical cord Wharton’s Jelly derived MSC (hUC-MSCs) were tested concurrently. At in vitro culture, both hUC-MSCs and hC-MSCs assumed spindle shape morphology with expression of typical MSC markers namely CD105, CD73, CD90 and CD44. Although, hUC-MSCs and hC-MSCs are identical in term of morphology and immunophenotype, yet hUC-MSCs harbored a higher cell growth as compared to the hC-MSCs. The inherent cardiac regenerative potential of both cells were further investigated with mRNA expression of ion channels. The RT-PCR results demonstrated that both MSCs were expressing a notable level of delayed rectifier-like K+ current (I KDR ) ion channel, yet the relative expression level was considerably varied between hUC-MSCs and hC-MSCs that Kv1.1(39 ± 0.6 vs 31 ± 0.8), Kv2.1 (6 ± 0.2 vs 21 ± 0.12), Kv1.5 (7.4 ± 0.1 vs 6.8 ± 0.06) and Kv7.3 (27 ± 0.8 vs 13.8 ± 0.6). Similarly, the Ca2+-activated K+ current (I KCa ) channel encoding gene, transient outward K+ current (I to ) and TTX-sensitive transient inward sodium current (I Na.TTX ) encoding gene (Kv4.2, Kv4.3 and hNE-Na) expressions were detected in both groups as well. Despite the morphological and phenotypical similarity, the present study also confirms the existence of multiple functional ion channel currents IKDR, IKCa, Ito, and INa.TTX in undifferentiated hUC-MSCs as of hC-MSCs. Thus, the hUC-MSCs can be exploited as a potential candidate for future cardiac regeneration.

Keywords

Mesenchymal stem cell Electrophysiology Cardiac resident stem cell and umbilical cord stem cell 

Abbreviations

MSCs

Mesenchymal stem cells

BM-MSCs

Bone marrow-derived MSCs

CSC

Cardiac stem cells

hC-MSC

Human cardiac MSC

hUC-MSCs

Human umbilical cord derived MSCs

Notes

Compliance with ethical standards

Conflict of interest

The authors express no conflicts of interest towards the publication of this paper.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Baskar Subramani
    • 1
    • 2
  • Sellamuthu Subbannagounder
    • 1
  • Sekar Palanivel
    • 3
  • Chithra Ramanathanpullai
    • 1
  • Sivakumar Sivalingam
    • 4
  • Azhari Yakub
    • 4
  • Manjunath SadanandaRao
    • 5
  • Arivudainambi Seenichamy
    • 6
  • Ashok Kumar Pandurangan
    • 7
  • Jun Jie Tan
    • 8
  • Rajesh Ramasamy
    • 9
    • 10
    Email author
  1. 1.Nichi-Asia Life Sdn Bhd.Petaling JayaMalaysia
  2. 2.Bharathiyar UniversityCoimbatoreIndia
  3. 3.Departments of ZoologyGovernment Arts College (Autonomous)SalemIndia
  4. 4.Cardiothoracic Surgery UnitNational Heart InstituteKuala LumpurMalaysia
  5. 5.Micro Therapeutic Research Labs Pvt. LtdChennaiIndia
  6. 6.Department of Veterinary Pathology and MicrobiologyUniversiti Putra MalaysiaSerdangMalaysia
  7. 7.Department of Pharmacology, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia
  8. 8.Regeneration Medicine Cluster, Advanced Medicine and Dental InstituteUniversiti Sains MalaysiaGeorge TownMalaysia
  9. 9.Stem Cell and Immunity Group, Immunology Laboratory Unit, Department Of Pathology, Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdangMalaysia
  10. 10.Stem Cell Research Laboratory, Genetic and Regenerative Medicine Research Center, Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdangMalaysia

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