Abstract
Cell based therapy (CBT) provides an alternative option to replace damaged myocardium and vasculature and restore cardiac contractility. Several cardiac and non-cardiac stem/precursor/progenitor cells have been used in both pre-clinical studies and clinical trials to regenerate the failing heart. CBT should be carried out by using cardiac lineage-committed stem cells that are physically capable of replacing lost native CMs and functionally integrate within the beating heart. This novel context has led to the re-appreciation of the therapeutic use of pluripotent embryonic stem cells (ESCs), unspecialized cells which can be indefinitely cultivated in vitro. The present chapter aims at addressing the basic issues regarding isolation, characterization and CM differentiation of mESCs and hESCs and at focusing on their advantages and disadvantages for cardiac regeneration.
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Moccia, F., Diofano, F., Rebuzzini, P., Zuccolo, E. (2016). Embryonic Stem Cells for Cardiac Regeneration. In: Madonna, R. (eds) Stem Cells and Cardiac Regeneration. Stem Cell Biology and Regenerative Medicine. Springer, Cham. https://doi.org/10.1007/978-3-319-25427-2_2
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