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Mimicking breast cancer-induced bone metastasis in vivo: current transplantation models and advanced humanized strategies

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Abstract

Bone metastasis is a complication that occurs in 80 % of women with advanced breast cancer. Despite the prevalence of bone metastatic disease, the avenues for its clinical management are still restricted to palliative treatment options. In fact, the underlying mechanisms of breast cancer osteotropism have not yet been fully elucidated due to a lack of suitable in vivo models that are able to recapitulate the human disease. In this work, we review the current transplantation-based models to investigate breast cancer-induced bone metastasis and delineate the strengths and limitations of the use of different grafting techniques, tissue sources, and hosts. We further show that humanized xenograft models incorporating human cells or tissue grafts at the primary tumor site or the metastatic site mimic more closely the human disease. Tissue-engineered constructs are emerging as a reproducible alternative to recapitulate functional humanized tissues in these murine models. The development of advanced humanized animal models may provide better platforms to investigate the mutual interactions between human cancer cells and their microenvironment and ultimately improve the translation of preclinical drug trials to the clinic.

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Acknowledgments

Some figures were produced using the image bank at www.servier.com with permission from Servier Medical Art. The work presented by the authors is supported by the Australian Research Council and the National Health and Medical Research Council. A.V.T. and B.M.H. are supported by the German Research Foundation (DFG HO 5068/1-1 to B.M.H.).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD, 4049, Australia

    Laure Thibaudeau, Boris M. Holzapfel, Anna V. Taubenberger & Dietmar W. Hutmacher

  2. Department of Obstetrics and Gynecology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg, Universitaetsstraße 21-23, 91054, Erlangen, Germany

    Verena M. Quent

  3. Orthopedic Center for Musculoskeletal Research, University of Wuerzburg, Brettreichstraße 11, 97072, Wuerzburg, Germany

    Boris M. Holzapfel

  4. Biotec TU Dresden, Tatzberg 47/49, 01307, Dresden, Germany

    Anna V. Taubenberger

  5. Institute of Pathology, Technical University Munich, Trogerstraße 18, 81675, Munich, Germany

    Melanie Straub

  6. George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 801 Ferst Drive Northwest, Atlanta, GA, 30332, USA

    Dietmar W. Hutmacher

  7. Institute for Advanced Study, Technical University Munich, Lichtenbergstraße 2a, 85748, Garching, Germany

    Dietmar W. Hutmacher

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  1. Laure Thibaudeau
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  4. Anna V. Taubenberger
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  6. Dietmar W. Hutmacher
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Correspondence to Laure Thibaudeau or Dietmar W. Hutmacher.

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Thibaudeau, L., Quent, V.M., Holzapfel, B.M. et al. Mimicking breast cancer-induced bone metastasis in vivo: current transplantation models and advanced humanized strategies. Cancer Metastasis Rev 33, 721–735 (2014). https://doi.org/10.1007/s10555-014-9499-z

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  • DOI: https://doi.org/10.1007/s10555-014-9499-z

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