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Circular RNA circMMP1 Contributes to the Progression of Glioma Through Regulating TGIF2 Expression by Sponging miR-195-5p

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Abstract

Glioma is characterized by high morbidity and mortality worldwide. Circular RNA (circRNA) matrix metallopeptidase 1 (circMMP1, hsa_circ_0024108) was reported to be increased in glioma. This study is designed to explore the role and mechanism of circMMP1 in glioma progression. CircMMP1, linear MMP1, microRNA-195-5p (miR-195-5p), and transforming growth factor-beta-induced 2 (TGIF2) level were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The protein levels of TGIF2, Beclin1, and p62 were examined by Western blot assay. Colony number, migration, invasion, and apoptosis were detected by Colony formation, transwell, and flow cytometry assays, severally. The binding relationship between miR-195-5p and circMMP1 or TGIF2 was predicted by starbase or Targetscan and then verified by a dual-luciferase reporter and RNA Immunoprecipitation (RIP) assays. The biological role of circMMP1 on glioma cell growth was examined by the xenograft tumor model in vivo. CircMMP1 and TGIF2 expression were upregulated, and miR-195-5p expression was downregulated in glioma tissues and cells. And the knockdown of circMMP1 could block colony formation, migration, and invasion and expedite apoptosis and autophagy in glioma cells. The mechanical analysis discovered that circMMP1 acted as a sponge of miR-195-5p to regulate TGIF2 expression. CircMMP1 knockdown suppressed cell growth of glioma in vivo. CircMMP1 boosted glioma progression partly by targeting the miR-195-5p/TGIF2 axis, suggesting a promising circRNA-targeted therapy for glioma treatment.

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Data Availability

All data generated or analyzed during this study are included in this article.

Abbreviations

TGIF2:

Transforming growth factor-beta-induced 2

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Acknowledgements

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Funding

This study was supported by Key Discipline Construction Project of Pudong Health Bureau of Shanghai (Grant No. PWZxk2017-23, PWYgf2018-05).

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Author notes

  1. Kuiming Zhang and Qi Wang are co-first authors. They are contributed equally to this work.

Authors and Affiliations

  1. Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China

    Kuiming Zhang & Qi Wang

  2. Department of Neurosurgery, Baoshan People’s Hospital, Baoshan, Yunnan, China

    Dehao Zhao

  3. Department of Neurosurgery, Nanyang Second General Hospital, No. 66 Jianshe East Road, Nanyang City, 473012, Henan Province, China

    Zhen Liu

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  1. Kuiming Zhang
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  2. Qi Wang
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  3. Dehao Zhao
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  4. Zhen Liu
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Contributions

KZ conceived and designed the experiments, performed the experiments, analyzed and interpreted the data, and wrote the paper. QW analyzed and interpreted the data and performed the experiments. DZ performed the experiments and analyzed the data. ZL analyzed and interpreted the data.

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Correspondence to Zhen Liu.

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The research related to human use has been complied with all the relevant national regulations and has been approved by the review board of Shanghai East Hospital.

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Zhang, K., Wang, Q., Zhao, D. et al. Circular RNA circMMP1 Contributes to the Progression of Glioma Through Regulating TGIF2 Expression by Sponging miR-195-5p. Biochem Genet 60, 770–789 (2022). https://doi.org/10.1007/s10528-021-10119-x

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  • DOI: https://doi.org/10.1007/s10528-021-10119-x

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