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Bulletin of Experimental Biology and Medicine

, Volume 163, Issue 4, pp 439–442 | Cite as

Effects of Miramistin and Phosprenil on Microbial Biofilms

  • T. A. DanilovaEmail author
  • G. A. Danilina
  • A. A. Adzhieva
  • A. G. Minko
  • T. N. Nikolaeva
  • V. G. Zhukhovitskii
  • A. V. Pronin
IMMUNOLOGY AND MICROBIOLOGY

Effects of Miramistin and Phosprenil on biofilms of S. pyogenes, S. aureus, E. coli, L. acidophilus, and L. plantarum were studied. Significant differences in the effects of these substances on mature biofilms of microorganisms and the process of their formation were observed. Miramistin had significant inhibiting effects on the forming of biofilms and on the formed biofilms of all studied microorganisms. Treatment with Miramistin inhibited biofilm formation by 2-3 times compared to the control. This effect was found already after using of Miramistin in the low doses (3.12 μg/ml). Inhibition of the growth of a formed biofilm was observed only after treatment with Miramistin in the high doses (25-50 μg/ml). Phosprenil in the high doses (15-30 mg/ml) inhibited the forming of biofilms, especially the biofilms of S. pyogenes and L. plantarum (by 3-4.5 times). Treatment of formed biofilms with the agent in doses of 6.0 and 0.6 mg/ml was associated with pronounced stimulation of its growth in S. pyogenes, S. aureus, and L. acidophilus.

Key Words

microorganisms biofilms Miramistin Phosprenil 

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References

  1. 1.
    Danilova TA, Danilina GA, Adzhieva AA, Minko AG, Alekseeva NV. Biofilm formation by group A Streptococci of various types and study of antibiotics effect on this process. Zh. Mikrobiol. Immunol. 2015(2):50-54. Russian.Google Scholar
  2. 2.
    Romanova JM, Didenko LV, Tolordava ER, Gintsburg AL. Biofilms of pathogenic bacteria and their role in chronization of infectious process. The search for the means to control biofilms. Vestn. Ross. Akad. Med. Nauk. 2011(10):31-39. Russian.Google Scholar
  3. 3.
    Tetz GV, Artemenko NK, Zaslavskaya NV, Tetz VV. Characteristics of bacterial biofilms during long-term culturing. Bull. Exp. Biol. Med. 2013;155(4):467-469.CrossRefPubMedGoogle Scholar
  4. 4.
    Bengmark S. Ecological control of the gastrointestinal tract. The role of probiotic flora. Gut. 1998;42(1):2-7.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Conley J, Olson ME, Cook LS, Ceri H, Phan V, Davies HD. Biofilm formation by group A streptococci: is there a relationship with treatment failure? J. Clin. Microbiol. 2003;41(9):4043-4048.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Hentzer M, Givskov M. Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. J. Clin. Invest. 2003;112(9):1300-1307.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Maddocks SE, Lopez MS, Rowlands RS, Cooper RA. Manuka honey inhibits the development of Streptococcus pyogenes biofilms and causes reduced expression of two fibronectin binding proteins. Microbiology. 2012;158(Pt 3):781-790.CrossRefPubMedGoogle Scholar
  8. 8.
    Marks LR, Mashburn-Warren L, Federle MJ, Hakansson AP. Streptococcus pyogenes biofilm growth in vitro and in vivo and its role in colonization, virulence and genetic exchange. J. Infect. Dis. 2014;210(1):25-34.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Nobbs AH, Lamont RJ, Jenkinson HF. Streptococcus adherence and colonization. Microbiol. Mol. Biol. Rev. 2009;73(3):407-450.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Nithyanand P, Beema Shafreen RM, Muthamil S, Karutha Pandian S. Usnic acid, a lichen secondary metabolite inhibits Group A Streptococcus biofilms. Antonie van Leeuwenhoek. 2015;107(1):263-272.CrossRefPubMedGoogle Scholar
  11. 11.
    O’Toole GA, Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceedsvia multiple, convergent signalling pathways: a genetic analysis. Mol. Microbiol. 1998;28(3):449-461.CrossRefPubMedGoogle Scholar
  12. 12.
    Pronin AV, Danilov LL, Narovlyansky AN, Sanin AV. Plant polyisoprenoids and control of cholesterol level. Arch. Immunol. Ther. Exp. (Warsz). 2014;62(1):31-39.CrossRefGoogle Scholar
  13. 13.
    Subramenium GA, Vijayakumar K, Pandian SK. Limonene inhibits streptococcal biofilm formation by targeting surface associated virulence factors. J. Med. Microbiol. 2015;64(8):879-890.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • T. A. Danilova
    • 1
    Email author
  • G. A. Danilina
    • 1
    • 3
  • A. A. Adzhieva
    • 1
  • A. G. Minko
    • 1
  • T. N. Nikolaeva
    • 2
  • V. G. Zhukhovitskii
    • 1
    • 3
  • A. V. Pronin
    • 2
  1. 1.Laboratory of Indication and Ultrastructural Analysis of MicroorganismsMoscowRussia
  2. 2.Laboratory of Natural Immunity, N. F. Gamaleya Federal Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian FederationMoscowRussia
  3. 3.I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian FederationMoscowRussia

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