Effects of Miramistin and Phosprenil on biofilms of S. pyogenes, S. aureus, E. coli, L. acidophilus, and L. plantarum were studied. Significant differences in the effects of these substances on mature biofilms of microorganisms and the process of their formation were observed. Miramistin had significant inhibiting effects on the forming of biofilms and on the formed biofilms of all studied microorganisms. Treatment with Miramistin inhibited biofilm formation by 2-3 times compared to the control. This effect was found already after using of Miramistin in the low doses (3.12 μg/ml). Inhibition of the growth of a formed biofilm was observed only after treatment with Miramistin in the high doses (25-50 μg/ml). Phosprenil in the high doses (15-30 mg/ml) inhibited the forming of biofilms, especially the biofilms of S. pyogenes and L. plantarum (by 3-4.5 times). Treatment of formed biofilms with the agent in doses of 6.0 and 0.6 mg/ml was associated with pronounced stimulation of its growth in S. pyogenes, S. aureus, and L. acidophilus.
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Danilova TA, Danilina GA, Adzhieva AA, Minko AG, Alekseeva NV. Biofilm formation by group A Streptococci of various types and study of antibiotics effect on this process. Zh. Mikrobiol. Immunol. 2015(2):50-54. Russian.
Romanova JM, Didenko LV, Tolordava ER, Gintsburg AL. Biofilms of pathogenic bacteria and their role in chronization of infectious process. The search for the means to control biofilms. Vestn. Ross. Akad. Med. Nauk. 2011(10):31-39. Russian.
Tetz GV, Artemenko NK, Zaslavskaya NV, Tetz VV. Characteristics of bacterial biofilms during long-term culturing. Bull. Exp. Biol. Med. 2013;155(4):467-469.
Bengmark S. Ecological control of the gastrointestinal tract. The role of probiotic flora. Gut. 1998;42(1):2-7.
Conley J, Olson ME, Cook LS, Ceri H, Phan V, Davies HD. Biofilm formation by group A streptococci: is there a relationship with treatment failure? J. Clin. Microbiol. 2003;41(9):4043-4048.
Hentzer M, Givskov M. Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. J. Clin. Invest. 2003;112(9):1300-1307.
Maddocks SE, Lopez MS, Rowlands RS, Cooper RA. Manuka honey inhibits the development of Streptococcus pyogenes biofilms and causes reduced expression of two fibronectin binding proteins. Microbiology. 2012;158(Pt 3):781-790.
Marks LR, Mashburn-Warren L, Federle MJ, Hakansson AP. Streptococcus pyogenes biofilm growth in vitro and in vivo and its role in colonization, virulence and genetic exchange. J. Infect. Dis. 2014;210(1):25-34.
Nobbs AH, Lamont RJ, Jenkinson HF. Streptococcus adherence and colonization. Microbiol. Mol. Biol. Rev. 2009;73(3):407-450.
Nithyanand P, Beema Shafreen RM, Muthamil S, Karutha Pandian S. Usnic acid, a lichen secondary metabolite inhibits Group A Streptococcus biofilms. Antonie van Leeuwenhoek. 2015;107(1):263-272.
O’Toole GA, Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceedsvia multiple, convergent signalling pathways: a genetic analysis. Mol. Microbiol. 1998;28(3):449-461.
Pronin AV, Danilov LL, Narovlyansky AN, Sanin AV. Plant polyisoprenoids and control of cholesterol level. Arch. Immunol. Ther. Exp. (Warsz). 2014;62(1):31-39.
Subramenium GA, Vijayakumar K, Pandian SK. Limonene inhibits streptococcal biofilm formation by targeting surface associated virulence factors. J. Med. Microbiol. 2015;64(8):879-890.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 4, pp. 436-439, April, 2017
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Danilova, T.A., Danilina, G.A., Adzhieva, A.A. et al. Effects of Miramistin and Phosprenil on Microbial Biofilms. Bull Exp Biol Med 163, 439–442 (2017). https://doi.org/10.1007/s10517-017-3823-x
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DOI: https://doi.org/10.1007/s10517-017-3823-x