We compared the effects of disinfectants on biofilms of 10 Pseudomonas aeruginosa strains isolated from different loci of patients with purulent-septic infections. Identification was carried out by standard bacteriological methods. To substantiate the prospects of using in hospitals and to assess the effect of disinfectants on P. aeruginosa biofilms, the following disinfectants were used in various concentrations: Sekusept Aktiv, A-DEZ, and Monitor Oxy. All clinical strains of P. aeruginosa showed the ability to form biofilms. Both oxygen-containing and quaternary ammonium compounds effectively inhibited the formation of biofilms. In more than 50% cases, disinfectants with different active ingredients did not destroy pre-formed of P. aeruginosa biofilms.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Grenkova TA, Sel’kova EP, Gusarova MP, Ershova ON, Aleksandrova IA, Sazykina SYu, Kurdyumova NV. Stability control of microorganisms to antibiotics, antiseptics and disinfectants. Epidemiol. Vaktsinoprof. 2014;(1):29-33. Russian.
Noskova OA, Savilov ED, Chemezova NN, Belkova NL. Antibiotic resistance of pathogens of generalized purulent septic infections in children. Epidemiol. Vaktsinoprof. 2020;19(6):56-61. https://doi.org/10.31631/2073-3046-2020-19-6-56-61. Russian.
Sergevnin VI, Kudryavtseva LG, Pegyshina OG, Volkova EO, Reshetnikova NI. Group incidence by purulent-septic infections of clebsiellous etiology in cardiosurgical patients. Epidemiol. Vaktsinoprof. 2020;19(1):90-98. https://doi.org/10.31631/2073-3046-2020-19-1-90-98. Russian.
Akturk E, Oliveira H, Santos S.B, Costa S, Kuyumcu S, Melo LDR, Azeredo J. Synergistic action of phage and antibiotics: parameters to enhance the killing efficacy against mono and dual-species biofilms. Antibiotics (Basel). 2019;8(3):103. https://doi.org/10.3390/antibiotics8030103
Amini Tapouk F, Nabizadeh R, Mirzaei N, Hosseini Jazani N, Yousefi M, Valizade Hasanloei MA. Comparative efficacy of hospital disinfectants against nosocomial infection pathogens. Antimicrob. Resist. Infect. Control. 2020;9(1):115. https://doi.org/10.1186/s13756-020-00781-y
Exner M, Bhattacharya S, Christiansen B, Gebel J, Goroncy-Bermes P, Hartemann P, Heeg P, Ilschner C, Kramer A, Larson E, Merkens W, Mielke M, Oltmanns P, Ross B, Rotter M, Schmithausen RM, Sonntag HG, Trautmann M. Antibiotic resistance: what is so special about multidrug-resistant Gramnegative bacteria? GMS Hyg. Infect. Control. 2017;12. Doc05. https://doi.org/10.3205/dgkh000290
Haney EF, Trimble MJ, Cheng JT, Vallé Q, Hancock REW. Critical assessment of methods to quantify biofilm growth and evaluate antibiofilm activity of host defence peptides. Biomolecules. 2018;8(2):29. https://doi.org/10.3390/biom8020029
Lineback CB, Nkemngong CA, Wu ST, Li X, Teska PJ, Oliver HF. Hydrogen peroxide and sodium hypochlorite disinfectants are more effective against Staphylococcus aureus and Pseudomonas aeruginosa biofilms than quaternary ammonium compounds. Antimicrob. Resist. Infect. Control. 2018;7:154. https://doi.org/10.1186/s13756-018-0447-5
Ma YX, Wang CY, Li YY, Li J, Wan QQ, Chen JH, Tay FR, Niu LN. Considerations and caveats in combating ESKAPE pathogens against nosocomial infections. Adv. Sci. (Weinh). 2019;7(1):1901872. https://doi.org/10.1002/advs.201901872
Montagna MT, Triggiano F, Barbuti G, Bartolomeo N, De Giglio O, Diella G, Lopuzzo M, Rutigliano S, Serio G, Caggiano G. Study on the in vitro activity of five disinfectants against nosocomial bacteria. Int. J. Environ. Res. Public Health. 2019;16(11):1895. https://doi.org/10.3390/ijerph16111895
Muhammad MH, Idris AL, Fan X, Guo Y, Yu Y, Jin X, Qiu J, Guan X, Huang T. Beyond risk: bacterial biofilms and their regulating approaches. Front. Microbiol. 2020;11:928. https://doi.org/10.3389/fmicb.2020.00928
Murray J, Muruko T, Gill CIR, Kearney MP, Farren D, Scott MG, McMullan G, Ternan NG. Evaluation of bactericidal and anti-biofilm properties of a novel surface-active organosilane biocide against healthcare associated pathogens and Pseudomonas aeruginosa biolfilm. PLoS One. 2017;12(8):e0182624. https://doi.org/10.1371/journal.pone.0182624
Nemchenko UM, Kungurtseva EA, Grigorova EV, Belkova NL, Markova YA, Noskova OA, Chemezova NN, Savilov ED. Simulation of bacterial biofilms and estimation of the sensitivity of healthcare-associated infection pathogens to bactericide Sekusept active. Klin. Lab. Diagn. 2020;65(10):652-658. https://doi.org/10.18821/0869-2084-2020-65-10-652-658
Teirlinck E, Xiong R, Brans T, Forier K, Fraire J, Van Acker H, Matthijs N, De Rycke R, De Smedt SC, Coenye T, Braeckmans K. Laser-induced vapour nanobubbles improve drug diffusion and efficiency in bacterial biofilms. Nat. Commun. 2018;9(1):4518. https://doi.org/10.1038/s41467-018-06884-w
Verderosa A.D, Totsika M, Fairfull-Smith KE. Bacterial biofilm eradication agents: a current review. Front. Chem. 2019;7:824. https://doi.org/10.3389/fchem.2019.00824
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 6, pp. 733-738, June, 2021
Rights and permissions
About this article
Cite this article
Grigorova, E.V., Nemchenko, U.M., Voropaeva, N.M. et al. Effect of Disinfectants with Different Active Ingredients on Biofilm Formation in Pseudomonas aeruginosa. Bull Exp Biol Med 171, 745–749 (2021). https://doi.org/10.1007/s10517-021-05308-y
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-021-05308-y