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Pseudomonas sesami sp. nov., a plant growth-promoting Gammaproteobacteria isolated from the rhizosphere of Sesamum indicum L.

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Abstract

A novel Gram-stain negative, aerobic, motile, rod-shaped bacterium was isolated from Sesame (Sesamum indicum L.) rhizosphere soil. Based on the 16S rRNA gene similarity value (99.4–98.6%) obtained with phylogenetically closely related strains and through analyses of their house keeping genes (atpD, infB and rpoB), the strain SI-P133T was delineated among the species of the genus Pseudomonas and was subjected to polyphasic taxonomic analysis. It was a chemoorganotroph which grew at wide range of temperature (4–45 °C), pH (5.5–9.5) and NaCl concentrations (0–7% (w/v). DNA–DNA hybridization values with closely related type strains DSM 9751T, DSM 19095T, DSM 21509T, ICMP 9151T and DSM 6929T ranged from 23.1 to 44.2%. The most abundant fatty acids were C16:0, C10:0 3-OH, summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C17:0 cyclo and C12:0 3-OH. The major isoprenoid quinone system was ubiquinone 9 (Q-9) and the G+C content was 61.3 mol%. The major polar lipids of the strain SI-P133T were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. With regard to prospective use in agriculture, plant growth-promoting properties of the strain were tested and plant growth-promotion was demonstrated under in vitro conditions. Based on the various polyphasic taxonomic traits analysed, the strain SI-P-133T was novel and placed within the genus Pseudomonas. Hence we propose a novel species named Pseudomonas sesami sp. nov., for which the type strain is SI-P133T (=NCIMB 14519T = KCTC 22518T).

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Acknowledgements

Authors are grateful to Professor Jean Euzéby for the support in the Latin etymology of the new species name. The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding of this research through the Research Group project No RGP-213.

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Authors and Affiliations

  1. Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore, Tamilnadu, 641 003, India

    Munusamy Madhaiyan & Selvaraj Poonguzhali

  2. Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore

    Munusamy Madhaiyan & Selvaraj Poonguzhali

  3. Department of Microbiology, Indira Gandhi College of Arts and Science, Kathirkamam, Pondicherry, 605009, India

    Venkatakrishnan Sivaraj Saravanan & Kumaran Selvapravin

  4. Addiriyah Chair for Environmental Studies, Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia

    Veeramuthu Duraipandiyan & Naif Abdullah Al-Dhabi

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  1. Munusamy Madhaiyan
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  2. Selvaraj Poonguzhali
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  3. Venkatakrishnan Sivaraj Saravanan
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  4. Kumaran Selvapravin
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  5. Veeramuthu Duraipandiyan
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  6. Naif Abdullah Al-Dhabi
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Correspondence to Munusamy Madhaiyan.

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Madhaiyan, M., Poonguzhali, S., Saravanan, V.S. et al. Pseudomonas sesami sp. nov., a plant growth-promoting Gammaproteobacteria isolated from the rhizosphere of Sesamum indicum L.. Antonie van Leeuwenhoek 110, 843–852 (2017). https://doi.org/10.1007/s10482-017-0859-x

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