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Regulation of ethylene levels in canola (Brassica campestris) by 1-aminocyclopropane-1-carboxylate deaminase-containing Methylobacterium fujisawaense

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Abstract

We report the presence of ACC deaminase in Methylobacterium fujisawaense and its lowering of ethylene levels and promotion of root elongation in canola seedlings under gnotobiotic conditions. To test a part of the previous model proposed for ACC deaminase producing bacteria with Methylobacterium, ACC levels and various enzyme activities were monitored in canola. Lower amounts of ACC were present in the tissues of seeds treated with M. fujisawaense strains than in control seeds treated with MgSO4. Though the increased activities of ACC synthase in the tissue extracts of the treated seedlings might be due to bacterial indole-3-acetic acid, the amount of ACC was reduced due to bacterial ACC deaminase activity. The activities of ACC oxidase, the enzyme catalyzing conversion of ACC to ethylene remained lower in M. fujisawaense treated seedlings. This consequently lowered the ethylene in plants and prevented ethylene inhibition of root elongation. Our results collectively suggest that Methylobacterium commonly found in soils, as well as on the surfaces of leaves, seeds, and in the rhizosphere of a wide variety of plants could be better exploited to promote plant growth.

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Abbreviations

ACC:

1-aminocyclopropane-1-carboxylate

ACO:

ACC oxidase

ACS:

ACC synthase

AMS:

Ammonium mineral salts

AVG:

l-α-(2-aminoethoxyvinyl) glycine hydrochloride

CFU:

Colony-forming units

IAA:

Indole-3-acetic acid

iPA:

Isopentenyladenosine

MTA:

5′-methylthioadenosine

PGPR:

Plant growth promoting rhizobacteria

PLP:

Pyridoxal phosphate

PPFMs:

Pink-pigmented facultative methylotrophic bacteria

PVPP:

Polyvinylpolypyrrolidone

SAM:

S-adenosyl methionine

t-ZR:

trans-Zeatin riboside

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Acknowledgments

This work was supported by grants from the Korea Research Foundation, under the Foreign Scientist and Engineers programme research awarded to M. Madhaiyan and Rural Development Administration (RDA), Republic of Korea. The authors wish to thank anonymous referees for their valuable comments on the manuscript.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, Chungbuk National University, 361-763, Cheongju, Chungbuk, Republic of Korea

    Munusamy Madhaiyan, Selvaraj Poonguzhali, Jeounghyun Ryu & Tongmin Sa

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  1. Munusamy Madhaiyan
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  2. Selvaraj Poonguzhali
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  3. Jeounghyun Ryu
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  4. Tongmin Sa
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Correspondence to Tongmin Sa.

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Madhaiyan, M., Poonguzhali, S., Ryu, J. et al. Regulation of ethylene levels in canola (Brassica campestris) by 1-aminocyclopropane-1-carboxylate deaminase-containing Methylobacterium fujisawaense . Planta 224, 268–278 (2006). https://doi.org/10.1007/s00425-005-0211-y

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