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Metabacillus rhizolycopersici sp. nov., Isolated from the Rhizosphere Soil of Tomato Plants

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Abstract

A Gram-positive, endospore-forming, rod-shaped and aerobic bacterium, with swarming and swimming motility, designated strain DBTR6T, was isolated from the rhizosphere soil of tomato plants. Strain DBTR6T grew at 20–45 ℃ (optimum 30–37℃), pH 4–9 (optimum 7–8) and at salinities from 0 to 5% (optimum 1%). Phylogenetic analysis using 16S rRNA gene sequences showed this strain belonged to the genus Metabacillus and was most closely related to Metabacillus litoralis DSM 16303 T (98.3%) and Metabacillus sediminilitoris MCCC 1K03777T (98.3%). The DNA G + C content of the genomic DNA was 36.4%. The digital DNA–DNA hybridization value between strain DBTR6T and reference strains M. sediminilitoris MCCC 1K03777T and “M. bambusae” BG109T were less than 70% (26.7% and 26.0%), and the average nucleotide identity score were less than 95% (78.55% and 78.38%), and the Amino Acid Identity values calculated were less than 96% (79.99% and 80.18%), respectively, suggesting that strain DBTR6T represented a novel species in the genus Metabacillus. Chemotaxonomic analysis showed that strain DBTR6T contained MK-7 as the major respiratory quinone. The predominant fatty acids (> 10.0%) were iso-C15:0, anteiso-C15:0 and C16:0. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), glycolipids (GL) and three unidentified lipids (L). Based on the differential physiological properties, biochemical characteristics and genotypic data, strain DBTR6T represents a novel species of the genus Metabacillus, for which the name Metabacillus rhizolycopersici sp. nov. is proposed. The type strain is DBTR6T (= ACCC 61900 T = JCM 35080 T).

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Abbreviations

ANI:

Average nucleotide identity

DDH:

DNA–DNA hybridization

GGDC:

Genome-to-Genome Distance Calculator

TSA:

Tryptic Soy Agar

R2A:

Reasoner’s 2A

NJ:

Neighbor joining

ML:

Maximum likelihood

MP:

Maximun parsimony

HPLC:

High-performance liquid chromatography

TLC:

Thin-layer chromatography

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Funding

Funding was provided by Key Technologies Research and Development Program of China (CN) (2019YFD1002000), The Agricultural Science and Technology Innovation Program (ASTIP No. CAAS-ZDRW202201), and Beijing Innovation Consortium of Special Crops Research System.

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Authors and Affiliations

  1. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Rong Ma, Xing Wang, Kyu Kyu Thin & Xiao-Xia Zhang

  2. Shanghai Academy of Landscape Architecture Science and Planning, Shanghai, 200232, People’s Republic of China

    Shan-Wen He & Ji-Gang Han

  3. Department of Zoology, University of Magway, Magway, Myanmar

    Kyu Kyu Thin

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  1. Rong Ma
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Contributions

All the authors contributed to the study conception and design. RM and SWH collected and analyzed samples. RM and XW drafted the manuscript. KKT and JGH edited the manuscript. XXZ designed experiment, edited, and approved the final manuscript. All authors read and approved the final version of the manuscript.

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Correspondence to Xiao-Xia Zhang.

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The draft genome and 16S rRNA gene sequences of strain DBTR6T have been deposited at GenBank/EMBL/DDBJ under accession numbers JAIQUM000000000 and OK274256 respectively.

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Ma, R., He, SW., Wang, X. et al. Metabacillus rhizolycopersici sp. nov., Isolated from the Rhizosphere Soil of Tomato Plants. Curr Microbiol 79, 302 (2022). https://doi.org/10.1007/s00284-022-02995-2

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