Microchimica Acta

, 186:618 | Cite as

Gold nanoparticle-based detection of dopamine based on fluorescence resonance energy transfer between a 4-(4-dialkylaminostyryl)pyridinium derived fluorophore and citrate-capped gold nanoparticles

  • Juanjuan PengEmail author
  • Na Zhou
  • Yang Zhong
  • Yaoquan Su
  • Lingzhi ZhaoEmail author
  • Young-Tae ChangEmail author
Original Paper


A colorimetric/fluorometric dual-signal assay is described for the determination of dopamine (DA). A nanoprobe was obtained by linking a 4-(4-dialkylaminostyryl)pyridinium derived fluorophore to citrate-capped gold nanoparticles (AuNPs). The fluorescence of the fluorophore is quenched by the AuNPs via fluorescence resonance energy transfe. In the presence of DA, the catechol group of DA can absorb on the surface of AuNPs to induce aggregation, which is accompanied by a color change from red to blue. The yellow fluorescence of the fluorophore with excitation/emission maximum at 365/570 nm is recovered. The dual-signal detection allows the quantitative analysis of DA within 300 μM by the colorimetric method and 80 μM by the fluorometric method. The detection limits for the colorimetric/fluorometric methods are 1.85 μM and 0.29 μM, respectively. Quantitative determination of DA in spiked urine samples was successfully demonstrated, with recoveries ranging from 98.2 to 106.0%.

Graphical abstract

A colorimetric/fluorometric dual-signal assay is described for the determination of dopamine by linking a fluorophore to gold nanoparticles. The dopamine causes aggregation of the nanoparticles to induce color change, which is followed by the recovery of the fluorescence.


Dual-signal probe Colorimetry Fluorometry Turn-on Urine samples 



We sincerely acknowledge the financial support of the National Natural Science Foundation of China (81701766 and 81702998).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3727_MOESM1_ESM.docx (2.8 mb)
ESM 1 (DOCX 2.76 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Natural Medicines, School of Basic Medical Sciences and Clinical PharmacyChina Pharmaceutical UniversityNanjingChina
  2. 2.School of Materials Science and Engineering, Hebei Provincial Key Laboratory of Traffic Engineering MaterialsShijiazhuang Tiedao UniversityShijiazhuangChina
  3. 3.Center for Self-assembly and ComplexityInstitute for Basic Science (IBS)PohangSouth Korea
  4. 4.Department of ChemistryPohang University of Science and TechnologyPohangSouth Korea

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