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Microparticle-enhanced Aspergillus ficuum phytase production and evaluation of fungal morphology in submerged fermentation

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Abstract

Phytase can be used in animal’s diets to increase the absorption of several divalent ions, amino acids and proteins and to decrease the excessive phosphorus release in manure to prevent negative effects on the environment. This study aimed to enhance the current submerged fungal phytase productions with a novel fermentation technique by evaluating the effect of the various microparticles on Aspergillus ficuum phytase production. It was observed that microparticles prevented bulk fungal pellet growth, decreased average fungal pellet size and significantly increased phytase activity in the submerged fermentation. Microbial structure imaging results showed that the average fungal pellet radius decreased from 800 to 500 and 200 µm by addition of 15 g/L aluminum oxide and talcum, respectively, in shake-flask fermentation. Also, addition of 15 g/L of talcum and aluminum oxide increased phytase activity to 2.01 and 2.93 U/ml, respectively, compared to control (1.02 U/ml) in shake-flask fermentation. Additionally, phytase activity reached 6.49 U/ml within 96 h of fermentation with the addition of 15 g/L of talcum, whereas the maximum phytase activity was only 3.45 U/ml at 120 h of fermentation for the control in the 1-L working volume bioreactors. In conclusion, microparticles significantly increased fungal phytase activity and production yield compared to control fermentation.

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Acknowledgments

This work was supported in part by the Turkish Ministry of Education which provided scholarship to Hasan Bugra Coban and the Pennsylvania Agricultural Experiment Station.

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Authors and Affiliations

  1. Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA, 16802, USA

    Hasan B. Coban & Ali Demirci

  2. The Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA

    Ali Demirci

  3. Department of Food Engineering, Akdeniz University, Antalya, 07058, Turkey

    Irfan Turhan

Authors
  1. Hasan B. Coban
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  2. Ali Demirci
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  3. Irfan Turhan
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Correspondence to Ali Demirci.

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Coban, H.B., Demirci, A. & Turhan, I. Microparticle-enhanced Aspergillus ficuum phytase production and evaluation of fungal morphology in submerged fermentation. Bioprocess Biosyst Eng 38, 1075–1080 (2015). https://doi.org/10.1007/s00449-014-1349-4

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  • DOI: https://doi.org/10.1007/s00449-014-1349-4

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