Bioprocess and Biosystems Engineering

, Volume 30, Issue 6, pp 419–427

Butanol production from wheat straw hydrolysate using Clostridium beijerinckii

Original Paper

Abstract

In these studies, butanol (acetone butanol ethanol or ABE) was produced from wheat straw hydrolysate (WSH) in batch cultures using Clostridium beijerinckii P260. In control fermentation 48.9 g L−1 glucose (initial sugar 62.0 g L−1) was used to produce 20.1 g L−1 ABE with a productivity and yield of 0.28 g L−1 h−1 and 0.41, respectively. In a similar experiment where WSH (60.2 g L−1 total sugars obtained from hydrolysis of 86 g L−1 wheat straw) was used, the culture produced 25.0 g L−1 ABE with a productivity and yield of 0.60 g L−1 h−1 and 0.42, respectively. These results are superior to the control experiment and productivity was improved by 214%. When WSH was supplemented with 35 g L−1 glucose, a reactor productivity was improved to 0.63 g L−1 h−1 with a yield of 0.42. In this case, ABE concentration in the broth was 28.2 g L−1. When WSH was supplemented with 60 g L−1 glucose, the resultant medium containing 128.3 g L−1 sugars was successfully fermented (due to product removal) to produce 47.6 g L−1 ABE, and the culture utilized all the sugars (glucose, xylose, arabinose, galactose, and mannose). These results demonstrate that C. beijerinckii P260 has excellent capacity to convert biomass derived sugars to solvents and can produce over 28 g L−1 (in one case 41.7 g L−1 from glucose) ABE from WSH. Medium containing 250 g L−1 glucose resulted in no growth and no ABE production. Mixtures containing WSH + 140 g L−1 glucose (total sugar approximately 200 g L−1) showed poor growth and poor ABE production.

Keywords

Wheat straw hydrolysate (WSH) Clostridium beijerinckii Butanol or ABE Productivity Yield 

References

  1. 1.
    Ezeji TC, Qureshi N, Karcher P, Blaschek HP (2006) Production of butanol from corn. In: Minteer S (ed) Alcoholic fuels. Taylor and Francis Group, LLC, CRC Press, Boca Raton, pp 99–122Google Scholar
  2. 2.
    Zverlov VV, Berezina O, Velikodvorskaya GA, Schwarz WH (2006) Bacterial acetone butanol production by industrial fermentation in Soviet Union: use of hydrolyzed agricultural waste fro biorefining. Appl Microbiol Biotechnol 71:587–597CrossRefGoogle Scholar
  3. 3.
    Formanek J, Mackie R, Blaschek HP (1997) Enhanced butanol production by Clostridium beijerinckii BA101 grown in semi-defined P2 medium containing 6 percent maltodextrin or glucose. Appl Environ Microbiol 63:2306–2310Google Scholar
  4. 4.
    Parekh M, Formanek J, Blaschek HP (1998) Development of cost-effective glucose-corn steep medium for the production of butanol by Clostridium beijerinckii. J Ind Microbiol Biotechnol 21:187–191CrossRefGoogle Scholar
  5. 5.
    Parekh M, Formanek J, Blaschek HP (1999) Pilot-scale production of butanol by Clostridium beijerinckii BA101 using low-cost fermentation medium based on corn steep water. Appl Microbiol Biotechnol 51:152–157CrossRefGoogle Scholar
  6. 6.
    Ezeji TC, Qureshi N, Blaschek HP (2005) Industrially relevant fermentation. In: Durre P (ed) Handbook on Clostridia. Taylor & Francis Group, CRC Press, Boca Raton, pp 797–812Google Scholar
  7. 7.
    Ladisch MR (1991) Fermentation derived butanol and scenarios for its uses in energy-related applications. Enzyme Microbial Technol 13:280–283CrossRefGoogle Scholar
  8. 8.
    Durre P (1998) New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation. Appl Microbiol Biotechnol 49:639–648CrossRefGoogle Scholar
  9. 9.
    Qureshi N, Li X-L, Hughes S, Saha BC, Cotta MA (2006) Butanol production from corn fiber xylan using Clostridium acetobutlycum. Biotechnol Prog 22:673–680CrossRefGoogle Scholar
  10. 10.
    Ezeji TC, Qureshi N, Blaschek HP (2004) Acetone–butanol–ethanol production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping. Appl Micriobiol Biotechnol 63:653–658CrossRefGoogle Scholar
  11. 11.
    Qureshi N, Maddox IS (1992) Application of novel technology to the ABE fermentation process: an economic analysis. Appl Biochem Biotechnol 34:441–448CrossRefGoogle Scholar
  12. 12.
    Qureshi N, Blaschek HP (2000) Economics of butanol fermentation using hyper-butanol producing Clostridium beijerinckii BA101. Trans Inst Chem Eng (Trans IChemE): (Chem Eng Res Design) 78:139–144Google Scholar
  13. 13.
    Qureshi N, Blaschek HP (2001) Evaluation of recent advances in butanol fermentation, upstream, and downstream processing. Bioproc Biosys Eng 24:219-226CrossRefGoogle Scholar
  14. 14.
    Tabka MG, Herpoel-Gimbert I, Monod F, Asther M, Sigoillot JC (2006) Enzymatic saccharification of wheat straw for bioethanol production by a combined cellulose xylanase and feruloyl esterase treatment. Enzyme Microb Technol 39:897–902CrossRefGoogle Scholar
  15. 15.
    Esteghlalian A, Hashimoto AG, Fenske JJ, Penner AH (1997) Modeling and optimization of dilute-sulfuric-acid pretreatment of corn stover, poplar, and switchgrass. Biores Technol 59:129–136CrossRefGoogle Scholar
  16. 16.
    Qureshi N, Blaschek HP (2005) Butanol production from agricultural biomass. In: Shetty K, Pometto A, Paliyath G (eds) Food biotechnology. Taylor & Francis Group plc, Boca Raton, pp 525–551Google Scholar
  17. 17.
    Jones DT, Woods DR (1986) Acetone–butanol fermentation revisited. Microbiol Rev 50:484–524Google Scholar
  18. 18.
    Ebener J, Qureshi N, Blaschek HP (2003) Corn fiber hydrolysis and fermentation to butanol using Clostridium beijerinckii BA101. In: 25th Biotechnology Symposium for Fuels and Chemicals, Breckenridge, May 4–7Google Scholar
  19. 19.
    Qureshi N, Ebener J, Ezeji TC, Dien B, Cotta MA, Blaschek HP (2007) Butanol production by Clostridium beijerinckii BA101. Part I: Use of acid and enzyme hydrolysed corn fiber. Biores Technol (Submitted)Google Scholar
  20. 20.
    Ezeji TC, Qureshi N, Blaschek HP (2007) Butanol production from agricultural residues: Impact of degradation products on Clostridium beijerinckii growth and butanol fermentation. Biotechnol Bioeng (in press)Google Scholar
  21. 21.
    Feedstuffs (2006) BP, Dupont to develop biofuels, June 26, p 20Google Scholar
  22. 22.
    Industrial Bioprocessing (2006) Dupont and BP will produce butanol for motor fuel, July issue, 28(7)Google Scholar
  23. 23.
    Ennis BM, Maddox IS (1985) Use of Clostridium acetobutylicum P262 for production of solvents from whey permeate. Biotechnol Lett 7:601–606CrossRefGoogle Scholar
  24. 24.
    Qureshi N, Blaschek HP (1999) Butanol recovery from model solution/fermentation broth by pervaporation: evaluation of membrane performance. Biomass Bioenergy 17:175–184CrossRefGoogle Scholar
  25. 25.
    Ezeji TC, Qureshi N, Blaschek HP (2003) Production of butanol by Clostridium beijerinckii BA101 and in-situ recovery by gas stripping. World J Microbiol Biotechnol 19:595–603CrossRefGoogle Scholar
  26. 26.
    Qureshi N, Li X, Hughes SR, Saha BC, Cotta MA (2006) Production of acetone butanol ethanol from corn fiber xylan using Clostridium acetobutylicum. Biotechnol Prog 22:673–680CrossRefGoogle Scholar
  27. 27.
    Maddox IS, Qureshi N, Roberts-Thomson K (1995) Production of acetone–butanol–ethanol from concentrated substrates using Clostridium acetobutylicum in an integrated fermentation-product removal process. Process Biochem 30:209–215CrossRefGoogle Scholar
  28. 28.
    Qureshi N, Maddox IS (2005) Reduction in butanol inhibition by perstraction: utilization of concentrated lactose/whey permeate by Clostridium acetobutylicum to enhance butanol fermentation economics. Official J Eur Fed Chem Eng (formerly Trans IChemE; Chem Eng Res Design): Food Bioproducts Processing C 83(C1):43–52Google Scholar
  29. 29.
    Qureshi N, Blaschek HP (1999) Production of acetone–butanol–ethanol (ABE) by hyper-butanol producing mutant strain of Clostridium beijerinckii BA101 and recovery by pervaporation. Biotechnol Prog 15:594–602CrossRefGoogle Scholar
  30. 30.
    Qureshi N, Maddox IS, Friedl A (1992) Application of continuous substrate feeding to the ABE fermentation: relief of product inhibition using extraction, perstraction, stripping and pervaporation. Biotechnol Prog 8:382–390CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Nasib Qureshi
    • 1
  • Badal C. Saha
    • 1
  • Michael A. Cotta
    • 1
  1. 1.United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization ResearchFermentation Biotechnology Research UnitPeoriaUSA

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