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Biobutanol production by batch and fed-batch fermentations from the green coconut husk hydrolysate using C. beijerinckii ATCC 10132

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Abstract

The present study evaluates the potential of green coconut fiber in the production of cellulosic butanol by ABE (acetone-butanol-ethanol) fermentation, using Clostridium beijerinckii ATCC 10132. Batch fermentation tests were carried out to investigate the influence of some nutrients (sources of nitrogen and mineral medium) in the supplementation of the enzymatic hydrolyzate of pretreated green coconut husks, as well as the effects of the initial concentration of sugars and the initial concentration of inoculum on the production of butanol among other fermentation products. The mode of conducting the process in a fed-batch was carried out to improve the yield and productivity of butanol. Using the enzymatic hydrolyzate obtained with 9 g/L of sugars (glucose + xylose), the ABE fermentation reached a yield of 0.53 g/g after 96 h, in which 3.4 g/L of butanol was obtained. The absence or insufficiency of some nutrients (minerals and phosphate buffer) resulted in low yields of ABE products, indicating the importance of adapting supplements to the chosen fermentation medium and the type of microorganism used. Fed-batch increased productivity in ABE fermentation (0.08 g/L.h for butanol). The results indicate that green coconut residues have energy potential and are low-cost raw materials for the biotechnological production of butanol, as an alternative and renewable product.

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Acknowledgements

This study was developed in the Graduate Program in Chemical Engineering at the Federal University of Rio Grande do Norte (PPGEQ / UFRN), Brazil. The authors would like to thank the Federal University of Rio Grande do Norte (UFRN), especially the Biochemical Engineering Laboratory, for the physical space and infrastructure made available for carrying out the experiments, and the AquaCoco Industry for donating the green coconut husks.

Funding

Financial support is granted by the CAPES (Coordination for the Improvement of Higher Education Personnel – Finance code 001).

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  1. Laboratory of Biochemical Engineering, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal/RN, 59078‑900, Brazil

    Petrúcia Karine Santos de Brito Bezerra, Juliana Chris Silva de Azevedo & Everaldo Silvino dos Santos

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  1. Petrúcia Karine Santos de Brito Bezerra
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  2. Juliana Chris Silva de Azevedo
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Contributions

Petrúcia Bezerra: conceptualization, methodology, data curation, writing—original draft preparation. Juliana Azevedo: visualization, reviewing, and editing. Everaldo Santos: writing—original draft preparation, funding acquisition, supervision writing.

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Correspondence to Everaldo Silvino dos Santos.

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Highlights

• Green coconut fiber was used to production of cellulosic butanol by ABE (acetone-butanol-ethanol) fermentation, using Clostridium beijerinckii ATCC 10132

• Fed-batch operation mode improved the yield and productivity of butanol

• The productivity of ABE fermentation reached 0.08 g/L.h for butanol in fed-batch

• Green coconut fiber is a low-cost residue for butanol production, an alternative, and renewable product

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de Brito Bezerra, P.K.S., de Azevedo, J.C.S. & dos Santos, E.S. Biobutanol production by batch and fed-batch fermentations from the green coconut husk hydrolysate using C. beijerinckii ATCC 10132. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04537-7

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