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Oecologia

, Volume 170, Issue 1, pp 111–122 | Cite as

Phenotypic and genetic differentiation among yellow monkeyflower populations from thermal and non-thermal soils in Yellowstone National Park

  • Ylva Lekberg
  • Beth Roskilly
  • Margaret F. Hendrick
  • Catherine A. Zabinski
  • Camille M. Barr
  • Lila FishmanEmail author
Population ecology - Original research

Abstract

In flowering plants, soil heterogeneity can generate divergent natural selection over fine spatial scales, and thus promote local adaptation in the absence of geographic barriers to gene flow. Here, we investigate phenotypic and genetic differentiation in one of the few flowering plants that thrives in both geothermal and non-thermal soils in Yellowstone National Park (YNP). Yellow monkeyflowers (Mimulus guttatus) growing at two geothermal (“thermal”) sites in YNP were distinct in growth form and phenology from paired populations growing nearby (<500 m distant) in non-thermal soils. In simulated thermal and non-thermal environments, thermal plants remained significantly divergent from non-thermal plants in vegetative, floral, mating system, and phenological traits. Plants from both thermal populations flowered closer to the ground, allocated relatively more to sexual reproduction, were more likely to initiate flowering under short daylengths, and made smaller flowers that could efficiently self-fertilize without pollinators. These shared differences are consistent with local adaptation to life in the ephemeral window for growth and reproduction created by winter and spring snowmelt on hot soils. In contrast, habitat type (thermal vs. non-thermal) explained little of the genetic variation at neutral markers. Instead, we found that one thermal population (Agrostis Headquarters; AHQ-T) was strongly differentiated from all other populations (all F ST > 0.34), which were only weakly differentiated from each other (all F ST < 0.07). Phenotypic differentiation of thermal M. guttatus, but little population genetic evidence of long-term ecotypic divergence, encourages further investigations of the potential for fine-scale adaptation and reproductive isolation across the geothermal gradient in Yellowstone.

Keywords

Life-history evolution Mating system evolution Reproductive isolation Mimulus guttatus Ecotype 

Notes

Acknowledgments

We thank Samantha Campbell, Karin Neff, Jill Grenon, and Kate Matney for assistance with plant care and data collection. We are grateful to Christie Hendrix and other Yellowstone National Park personnel for logistical support and permission for work in the Park. Financial support was provided by National Science Foundation grants DBI-0328326 and DEB-0918902 to L.F., and by the Thermal Biology Institute and the Montana Space Grant Consortium to Y.L. and C.A.Z. B.R. was supported in part by fellowships from the Montana Integrative Learning Experience for Students (MILES) program, through a grant to The University of Montana from the Howard Hughes Medical Institute-Undergraduate Science Education Program; Grant No. 52005905. All experiments were conducted in accordance with current US law. The authors declare that they have no conflict of interest.

Supplementary material

442_2012_2297_MOESM1_ESM.doc (884 kb)
Supplementary material 1 (DOC 884 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Ylva Lekberg
    • 1
    • 2
    • 3
  • Beth Roskilly
    • 4
  • Margaret F. Hendrick
    • 4
  • Catherine A. Zabinski
    • 1
  • Camille M. Barr
    • 4
  • Lila Fishman
    • 4
    Email author
  1. 1.Department of Land Resources and Environmental SciencesMontana State UniversityBozemanUSA
  2. 2.MPG RanchMissoulaUSA
  3. 3.Department of Ecosystem and Conservation SciencesUniversity of MontanaMissoulaUSA
  4. 4.Division of Biological SciencesUniversity of MontanaMissoulaUSA

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