Nucleo-cytoplasmic transport of TDP-43 studied in real time: impaired microglia function leads to axonal spreading of TDP-43 in degenerating motor neurons
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Transactivating DNA-binding protein-43 (TDP-43) deposits represent a typical finding in almost all ALS patients, more than half of FTLD patients and patients with several other neurodegenerative disorders. It appears that perturbation of nucleo-cytoplasmic transport is an important event in these conditions but the mechanistic role and the fate of TDP-43 during neuronal degeneration remain elusive. We have developed an experimental system for visualising the perturbed nucleocytoplasmic transport of neuronal TDP-43 at the single-cell level in vivo using zebrafish spinal cord. This approach enabled us to image TDP-43-expressing motor neurons before and after experimental initiation of cell death. We report the formation of mobile TDP-43 deposits within degenerating motor neurons, which are normally phagocytosed by microglia. However, when microglial cells were depleted, injury-induced motor neuron degeneration follows a characteristic process that includes TDP-43 redistribution into the cytoplasm, axon and extracellular space. This is the first demonstration of perturbed TDP-43 nucleocytoplasmic transport in vivo, and suggests that impairment in microglial phagocytosis of dying neurons may contribute towards the formation of pathological TDP-43 presentations in ALS and FTLD.
KeywordsAmyotrophic lateral sclerosis (ALS) Motor neuron disease (MND) Neurodegeneration Microglia TDP-43 Pathological spreading Zebrafish
Pathological deposition of protein aggregates characterises a large group of neurodegenerative diseases in which the key event is considered to be either the malformation of a key protein, or the failing of a crucial metabolic cascade, leading to the generation of a pathological proteinaceous agent that can self-propagate and result in gain- or loss-of-function progressing toward cell death .
An aggregating proteinopathy involving the TAR DNA-binding protein (TDP-43) provides a molecular link between two progressive neurodegenerative diseases: amyotrophic lateral sclerosis (ALS) of the motor cortex, brainstem and spinal cord, and frontotemporal lobar degeneration (FTLD) of the frontal, insular, and temporal cortex (non-SOD1 ALS and non-tau FTLD, termed ALS-FTLD; [3, 13, 15, 22, 43]).
In biochemical and cell culture models, TDP-43 has been identified as spontaneously aggregation prone [1, 27]. This propensity has been localised to a low-complexity domain in the C-terminal region that may play a role in RNA binding, granule formation, and/or liquid–liquid phase separation to subserve transcription dynamics and mRNA transport [6, 12, 32, 34, 55, 56]. It is hypothesised that inherited or spontaneous mutation of the encoding gene, TARDBP, or of an interacting partner, upsets the balance between aggregation and dissolution of TDP-43 [14, 71], which may be differentially regulated under changing conditions, including during the cellular stress/death response [7, 30].
TDP-43 also appears to display self-propagating behaviour. Feiler et al.  found that naive cultured mouse neurons will internalise tagged, oligomerized TDP-43 from the culture medium of transfected neurons. This was mediated by the packaging of TDP-43 into microvesicles or exosomes, which were more effective at transmission than free protein. Linking this phenomenon to clinical observations, when cell culture models were seeded with phosphorylated TDP-43+ve insoluble fractions from ALS and FTLD patient brain or spinal cord, there was a significant increase in intracellular endogenous TDP-43 aggregation and subsequent cell death [44, 57]. To follow on from this we examined how motor neuron injury and subsequent degeneration mobilises TDP-43 in vivo and may contribute to pathogenicity in ALS.
We have developed an in vivo transgenic model to visualise subcellular compartments of individual motor neurons in zebrafish spinal cord. Using this new model, we have expressed fluorophore-tagged human TDP-43 within individual spinal motor neurons to observe the intraneuronal localisation of TDP-43 over time. After confirming that the subcellular localisation of TDP-43 is the same in zebrafish and human, we discovered in vivo that TDP-43 concentrated in highly dynamic nuclear granules with droplet-like properties. By application of a cell-specific UV injury, we were able to induce neuronal degeneration, within which the fate of TDP-43 could be precisely followed. We found that motor neurons underwent a stereotyped neurodegenerative process with structural changes closely mirroring those of degenerating neurons in human tissue. We further report upon live-imaging evidence for different pathways of TDP-43 release and distribution; as aggregated fragments, diffuse in cytoplasm, as a component of pyknotic or karryhorexic nuclear fragmentation, anterograde axonal diffusion or as a component of microglial uptake.
Materials and methods
Zebrafish (Danio rerio) were maintained under standard conditions . Experiments were conducted under Macquarie University Animal Ethics and Biosafety approvals (2012/050 and 2015/033; 5201401007). Larvae were raised in E3 medium at 28 °C on a 14:10 light:dark cycle. 1-Phenyl-2-thiourea (PTU) was added to the E3 medium at 24 hpf at 0.005% to inhibit pigmentation. All experiments in this study were conducted at 3–5 days post-fertilisation (dpf).
Tg(-3mnx1:kalTA4-4xnrUAS-E1b-H2B-mCerulean3-P2A-mKO2-CAAX) (MQ13) was generated using recombined p5E-3mnx1 , pME: kalTA4-4xnrUAS-E1b, p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX , and pTol2pA2 . The pME:kalTA4-4xnrUAS-E1b construct was generated from PCR amplification of EcoRI- and Xho1-flanked kalTA4, and Xho1- and Sal1-flanked 4xnrUAS sequences from pME:kalTA4 and pME:4xnrUAS vectors, respectively (both vectors were a kind gift from the Parton Lab, University of Queensland). A Sal1- and Spe1-flanked E1b minimal promoter was added to the 3ʹ end and the construct subcloned into a lab derived pDONR 221 (Invitrogen) vector with EcoRI and Spe1 sites. The codon-optimised H2B–mCerulean3–P2A–mKOFP2–CAAX sequence was ordered from GeneArt and recombined into pDONRP2R-P3 (Invitrogen) to create the p3E–H2B–mCerulean3–P2A–mKOFP2–CAAX . It incorporated a nuclear-localised mCerulean3 fluorophore and a membrane-localised mKOFP2 (mKO2) fluorophore expressed in motor neurons.
Tg (-3mnx1:eGFP-HsaTDP-43WT) was generated using recombined p5E-3mnx1 (#74632) , pME-EGFP , p3E-HsaTDP-43 and pTol2pA2 . The p3E-HsaTDP-43 was generated by subcloning a BamHI–Spe1-flanked gene string encoding HsaTDP-43 (GeneArt) into the BamHI–Spe1 sites of p3E-MCS (#75174) .
Transgenic lines were generated by co-injection of the Tol2-flanked constructs and transposase .
PU.1 (spi1b) morpholino
PU.1 (spi1b)-targeted active MO: 5ʹ-GATATACTGATACTCCATTGGTGGT-3ʹ
PU.1 (spi1b) mismatch MO: 5ʹ-GATAAACTGTTACTCGATTGCTGGT-3ʹ
Notes on morpholino and zebrafish PU.1 orthologues spi1a/b
An in silico analysis showed that for the two zebrafish orthologues of PU.1, spi1a and spi1b, the morpholino above contains a matching sequence only for spi1b (D. rerio, GRCz10). Given the efficacy of the morpholino in this and previous studies [25, 48, 49, 50] knockdown of spi1b appears sufficient to induce primitive monocyte lineage inhibition in the zebrafish.
Imaging and UV injury induction
Imaging was conducted on a Leica SP5 confocal microscope. mTagBFP was excited by 405-nm laser diode, which was also used at higher power to induce UV injury . eGFP was excited by an argon laser at 488 nm. mKO2-CAAX and mCherry-CAAX were excited by a Leica white-light laser at 552 and 587 nm, respectively. Imaging for Fig. 2 and Online Resources 1 and 6 was conducted with an 8 kHz Leica resonance scanner. Objectives used were a Leica 40x HCX APO L U-V-I water immersion and a Leica 63X HC APO U-V-I CS2 water immersion.
Imaging processing was conducted in ImageJ (1.51n). Deconvolution was applied for Figs. 1, 2, 4, 5, 6 and 7. Point spread functions were generated by the Richards and Wolf algorithm  as implemented in the PSF generator plugin for ImageJ . Richardson–Lucy deconvolution was carried out in the DeconvolutionLab plugin for ImageJ . A mean filter of radius 1 was applied to Figs. 4 and 6.
3D volume analysis of TDP-43 nuclear/cytoplasm ratio
The ratio of nuclear to cytoplasmic eGFP-TDP43WT over the 3D neuron volume was derived by first establishing a separation of voxels containing eGFP-TDP43WT alone (cytoplasm) or both eGFP-TDP43WT and H2B-mCerulean3 (nucleus) using a custom macro in ImageJ. Fluorescence grey value of each voxel was regarded as an indication of fluorophore concentration and the voxel grey value was summed over each set. The neurons used in this analysis were imaged from the same crossing and with identical microscope excitation and collection settings.
3D volume analysis of TDP-43 accumulations
The volume measurements of the nuclear accumulations of eGFP-TDP43WT were derived by automatic 3D object recognition in ImageJ. 205-µm × 51-µm × 24-µm z-stacks were acquired in the spinal cord of three larvae expressing mnx1-driven eGFP-TDP43WT from the same crossing. Object counting was conducted with the Object Counter3D plugin in ImageJ (plugin authors are F. Cordelières and J. Jackson. The plugin is available at http://rsbweb.nih.gov/ij/plugins/track/objects.html).
Axonal eGFP localisation
For the analysis of eGFP in the axon before and after UV injury of Fig. 7 and Online Resource 9, the Simple Neurite Tracer plugin for ImageJ (v3.1.3, ) was used to generate a neurite vector and gather individual segment and whole neurite fluorescence intensity measures.
For the baseline of eGFP signal present in uninjured motor neuron axons of Supplementary Figure 4 (Online Resource 10), an 8-µm segmented line was drawn along the axon path from the hillock in Z-projected stacks, as visualised by the mKO2-CAAX fluorophore. The mean grey value was measured in the voxels falling on this line for both mKO2-CAAX and eGFP. To demonstrate that any eGFP signal was not lost to a process of background subtraction, we sampled two 8-µm lines in regions of the same image without a positively expressing cell to determine the background levels for each fluorophore in each image. These background values were averaged and reported to give the background value used for comparison to the axon path. The neurons used in this analysis were imaged from the same crossing and with identical microscope excitation and collection settings.
The software R (3.3.2) was used for statistical analyses. Differences between the means were evaluated using ANOVAs and post hoc Tukey tests. For all statistical tests, significance was taken as P < 0.05. Unless otherwise indicated, data values are presented as the mean ± standard error of the mean (SEM). Graphs were generated in R (3.3.2) and Microsoft Excel 2016.
Visualising the distribution of TDP-43 within spinal motor neurons using sub-compartment-specific reporters
In the motor neurons of the zebrafish spinal cord, eGFP-TDP43WT was observed to be strongly concentrated in the nucleus while relatively diffuse in the cytosol (Figs. 1biv, 2). This is in keeping with qualitative observations in human tissue section [43, 61], mouse tissue [39, 54] and cell culture models [4, 64]. To quantify this distribution, we assayed the relative distribution of eGFP-TDP43WT across the nucleus and cytoplasm over the entire cell body as a 3D volume, as determined by comparison with the co-expressed nuclear marker, H2B-mCerulean3 (see “Materials and methods”). We found that 78.2% of eGFP-TDP43WT localised to the nucleus (± 4.6%, n = 12 neurons in 4 larvae). This percentage is in line with a ~ 85% nuclear localisation reported previously using western blot quantitation of nuclear/cytoplasmic fractions from cultured hippocampal neurons .
Visualisation of dynamic TDP-43 granula in the nucleus of spinal motor neurons
The nucleus contained eGFP-TDP43WT in the form of discrete fluorescent “granula”. Automatic 3D object analysis of the TDP-43 aggregates revealed a volume range of 0.023–5.7 µm3, with 83% falling in the range of 0.023–0.048 µm3 (132 accumulations in three larvae, Supplementary Fig. 1 (Online Resource 10)). Live imaging revealed that these TDP-43 granula were not stationary but showed highly dynamic movements within the nucleus (Online Resource 1). Using high-frequency image acquisition (8 kHz resonance scanner, image every 50 s), droplet-like interactions between the accumulations could be observed (Fig. 2 and Online Resource 1). These may be indicative of TDP-43 incorporation in nuclear phase-separation compartmentalisation [60, 70].
Targeted UV-mediated injury in motor neurons induced a local microglial response including phagocytosis of intracellular TDP-43
We have previously demonstrated that application of a UV laser (405 nm) can selectively induce injury and cell death in zebrafish motor neurons in vivo [40, 41]. The UV laser beam can be focused to a narrow column of subcellular width. Accordingly, as shown in experiments with spinal cord motor neurons expressing the photoconvertible Kaede fluorophore, a single motor neuron can be precisely targeted . To further characterise the UV-mediated cellular damage in this in vivo model, we examined the bleaching curves in the regions immediately neighbouring the laser beam. Bleaching did occur around the target column; however, it was attenuated by 50% within 10 µm (approx. one cell width) and nearly 80% within 20 µm [Supplementary Fig. 2 (Online Resource 10)]. This steep power attenuation in the immediate surroundings of the laser beam supports the view that our method can be used to inflict very precise subcellular damage to selectively induce degeneration of a TDP-43-expressing neuron without inducing any significant injury to surrounding cells.
To examine the response of spinal microglia to the degeneration of a single spinal motor neuron, we generated transgenic fish that co-express cytoplasmic mTagBFP in motor neurons  and mCherry-CAAX in macrophages/microglia (mpeg1.1 promoter, ), respectively. Using DNA injections of eGFP-TDP43WT into these larvae we achieved expression of tagged TDP-43 in individual spinal motor neurons that allowed precise visualisation of TDP-43 distribution after laser ablation (n = 7 motor neurons in 6 larvae).
In three experiments in which we observed microglia phagocytosis of targeted neurons, the microglia ingested TDP-43 as part of their phagocytic response. Visualising the localisation of eGFP-TDP43WT during this process showed that during laser-induced neuronal degeneration microglia internalised TDP-43 predominantly as a component of the neuron nucleus. The fluorescence was lost rapidly (between frames, < 4 min) in the microglial phagosome, likely due to lysosomal fusion and rapid acidification of the organelle . This is reminiscent of the finding in the zebrafish tectum that microglia phagocytosis occurs early in the cell death process, usually preceding the binding of extracellular AnnexinV [48, 62] and suggests that microglia play an important role in phagocytosing degenerating motor neurons and intraneuronal TDP-43.
Depletion of microglia revealed the sequence of neurodegeneration and TDP-43 redistribution
To fully elucidate the fate of TDP-43 in a dying motor neuron in vivo, we inhibited microglial phagocytosis by morpholino targeting of PU.1 (zebrafish spi1b, see “Materials and methods”). PU.1 is a well-characterised transcription factor and essential for monocyte to macrophage lineage progression, and knockdown of PU.1 has been shown to reliably cause macrophage deficiency in zebrafish [25, 48, 49, 50].
Larvae were injected with a PU.1-targeted morpholino or a 4-bp-mismatch control and screened for effective macrophage knockdown as well as common morpholino-associated developmental abnormalities . Specific morpholino-induced abnormalities were not observed [Supplementary Fig. 3a (Online Resource 10)] and macrophage knockdown was confirmed at 72 h post-fertilisation (hpf) [Supplementary Fig. 3b (Online Resource 10)]. Repopulation of macrophages in these knockdown larvae had begun at 120 hpf.
Importantly, during the phase of cellular expansion, eGFP-TDP43WT rapidly redistributed from the nucleus to the cytoplasm (Figs. 4b, 5b). This process was confirmed using high-frequency image acquisition (8 kHz resonance scanner sequence, Online Resource 6). The nucleus remained visible as the brightest accumulation of eGFP-TDP43WT in either a condensed form (pyknotic) (Fig. 4biii) or it became fragmented (karyorrhexic) (Fig. 5 bii–iv). In the case of pyknosis, the remaining core of nuclear eGFP-TDP43WT persisted initially and disintegrated slowly thereafter (see next section).
TDP-43 fragments are released by dying motor neurons
Prior to the expansion and dissolution of the degenerating neuron, and in the absence of phagocytosing microglia, we occasionally observed fluorescently tagged fragments moving from the cytoplasm into the extracellular space. As shown in Fig. 6 and Online Resource 7, these fragments occasionally contained eGFP-TDP43WT. After expansion and dissolution, and after the nucleus had assumed a pyknotic morphology, the remaining core of eGFP-TDP43WT could be observed to gradually fragment and disperse (Fig. 6cvi and best illustrated live in Online Resource 7).
Axonal mislocalisation of TDP-43 prior to cellular disintegration
In addition to morphological alterations of the cell body, we also observed beading and disintegration of the axon as a sequela of UV-induced degeneration of spinal motor neurons (Online Resource 8). To follow up on this, we investigated whether the mislocalisation of TDP-43 into the cytoplasm was accompanied by an increase in axonal TDP-43. To establish a baseline, we examined eGFP-TDP43WT distribution in the axons of uninjured neurons in mKO2-CAAX co-expressing larvae. eGFP-TDP43WT was not detected along the axon proximal to the cell body [n = 8 neurons in three larvae, Supplementary Fig. 4 (Online Resource 10)]. Similarly, for the motor neurons targeted by UV to induce degeneration, such as the neuron demonstrated in Fig. 7, no eGFP-TDP43WT was detectable along the motor axon from the hillock to its conjunction with the axon bundle leading down into the myotome, whereas mTagBFP was present throughout the cytoplasm and along the axon. However, in two of the ten motor neurons that underwent UV-induced degeneration, we observed that the process of cellular expansion was accompanied by a rapid movement of eGFP-TDP43WT into the axon. The process is illustrated in Fig. 7 and Online Resource 9, both visually and by plotting the eGFP fluorescence intensity along the 3D traced axon over time. Following injury, the mean eGFP-TDP43WT intensity along the axon increased during the process of degeneration before spiking rapidly ~ 5 h after the initial UV stress (but prior to dissolution as described above). The observation of inducible spreading of TDP-43 into axons in a living organism points to the existence of a potential molecular mechanism for progressive transmission and redistribution of pathological TDP-43 to neighbouring and distal neurons within the motor system.
The subcellular redistribution of TDP-43 from the nucleus to the cytoplasm in injured motor neurons (when microglia were depleted and, therefore, the cells were not rapidly phagocytosed) was especially striking, and this study represents the first real-time in vivo observation of the phenomenon. This redistribution of TDP-43 to the cytoplasm has been suggested to represent a landmark feature of the onset and progression of ALS/FTLD [23, 43], and in cell culture the disrupted trafficking of TDP-43 is a precursor to the formation of cytoplasmic TDP-43 inclusions . Previously, Sato et al.,  have reported the cytoplasmic mislocalisation of TDP-43 in response to neuronal stress in a mouse ligation model. Our observations provide in vivo support for the hypothesis that cytoplasmic accumulation of TDP-43 in motor neurons could occur as a direct downstream consequence of neuronal stress or injury. It is important to point out that in our experiments microglia did not directly prevent the cytoplasmic mislocalisation of TDP-43 in UV-injured motor neurons, rather this process was circumvented by the microglial phagocytosis of the degenerating neuron. This raises an intriguing question—why is TDP-43 pathology limited to ALS/FTLD and not other neurodegenerative diseases (such as Alzheimer’s, Parkinson’s and Huntington diseases)? It is possible that the TDP-43 redistribution that we have observed is elicited by specific types of neuronal stress/injury (such as UV that was used in this study). Alternatively, it is possible that microglia are specifically defective in their phagocytic function in ALS/FTLD (and not other diseases), therefore, allowing intracellular TDP-43 redistribution to occur. These are important directions for future follow-up. Interestingly, Spiller and colleagues recently demonstrated the selective uptake of TDP-43 by reactive microglia in mice, and that this process is likely to play an important role in clearing pathological (mislocalised) TDP-43 from neurons .
Significant recent interest in the field has focussed on the potential cell-to-cell transfer of TDP-43 and/or associated proteins, and the possibility that this might facilitate propagation of the disease process through the motor system. In vitro, the intercellular transfer of TDP-43 has been demonstrated [21, 47, 57, 67]. However, whether this also occurs in vivo remains an open question. In our study, we observed two pathways for transfer of TDP-43 from the originating dying neuron: uptake by microglia via direct phagocytosis and transfer into the surrounding neuropil.
UV injury-induced degeneration resulted in a phagocytic response by spinal microglia, which took up predominantly nuclear TDP-43. This represents the first in vivo demonstration of TDP-43 uptake by microglia as well as the prevention of TDP-43 spreading by normal microglia. Further study into the fate of TDP-43 and whether microglial function is altered following TDP-43 uptake in the zebrafish model may help expand upon the early evidence for microglial involvement in the progression of ALS/FTLD [8, 35, 46]. For example, phagocytosis of degradation-resistant TDP-43 aggregates  may disrupt the lysosomal pathway in microglia and, therefore, impair their ability for clearance of TDP-43. Interestingly, Paolicelli et al. have recently reported that TDP-43 depletion in Cx3Cr1-expressing cells (including microglia) led to an increase of phagocytosis and elevated transcription of lysosomal components . This suggests that both endogenous and exogenously acquired TDP-43 may alter the functional behaviour of microglia, and contribute towards the progression of ALS/FTLD.
Depletion of microglia through PU.1 knockdown allowed us to visualise intracellular spreading of TDP-43. In fact, we observed at least three pathways for TDP-43 dispersal following motor neuron degeneration after injury: this included diffusion of TDP-43 from the nucleus to the cytoplasm after neuronal swelling, compartmentalised TDP-43 release in discrete quanta, and the breakdown of homeostatic TDP-43 exclusion from the axon. Concerning the interesting axonal spreading of TDP-43 it is important to note that we consistently did not detect any eGFP-TDP43WT inside axons of healthy spinal motor neurons. Neuronal cell cultures and drosophila imaging have identified axonal TDP-43 as a component of transport vesicles in keeping with a role as a chaperone of RNA transport [2, 19]. We frequently observed the cytoplasmic mTagBFP and the CAAX-tagged membrane-bound mKO2 fluorophores incorporated into transport vesicles moving antero- as well as retro-grade along the axon (data not shown). However, we never observed this active transport for eGFP-TDP43WT. This difference indicates the intriguing possibility that the incorporation of TDP-43 into transport vesicles may be differentially regulated between neuronal cell types, in an in vivo setting (i.e. cell culture vs vertebrate spinal cord) or in distinct environmental or developmental contexts.
This study provides the first live-imaging data to support axonal-mediated spreading of TDP-43 [9, 45]. In combination with observations obtained in cell cultures suggesting TDP-43 transfer via the axonal membrane , this provides evidence that axonal TDP-43 might be an important mediator of ALS/FTLD pathology. It is worth noting that alongside our observations of spinal microglia engulfment of motor neuron cell bodies in this study and in two previous studies [40, 41], we have never observed microglial phagocytosis of degenerating axons. Given that microglial neuronophagia preceded TDP-43 nucleo-cytoplasmic transport and that the anterograde movement of TDP-43 was a late process in neuron degeneration (that we could observe best when microglia were depleted), it is reasonable to conclude that rapid microglial phagocytosis of injured motor neuron somata is an effective mechanism for the prevention of TDP-43 spread.
Taken together, nucleo-cytoplasmic transport of TDP-43 was studied in real time in the living spinal cord, and revealed that depletion of functional microglia leads to axonal and extracellular spreading of the pathological protein. This provides new insight into the potential direct role of microglia as well as pathways for protein distribution in the cascade of cellular and molecular events that cause ALS and FTLD.
The authors would like to thank Thomas Hall in the Parton Lab at the University of Queensland for providing the kalTA4 and 4xnrUAS sequences. We also thank Leica Microsystems and in particular Stephen Thompson for their support. We wish to thank the Snow Foundation for their generous support towards establishing the transgenic zebrafish facility at Macquarie University.
Compliance with ethical standards
This work was supported by MNDRIA (GIA 1838, GIA 1638—Cure for MND Research Grant), ARC (DP150104472) and donations made towards MND research at Macquarie University.
Conflict of interest
The authors declare that they have no conflicts of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. This article does not contain any studies with human participants performed by any of the authors.
- 1.Afroz T, Hock EM, Ernst P, Foglieni C, Jambeau M, Gilhespy LAB, Laferriere F, Maniecka Z, Plückthun A, Mittl P, Paganetti P, Allain FHT, Polymenidou M (2017) Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation. Nat Commun 8:45. https://doi.org/10.1038/s41467-017-00062-0 CrossRefPubMedPubMedCentralGoogle Scholar
- 2.Alami NH, Smith RB, Carrasco MA, Williams LA, Winborn CS, Han SSW, Kiskinis E, Winborn B, Freibaum BD, Kanagaraj A, Clare AJ, Badders NM, Bilican B, Chaum E, Chandran S, Shaw CE, Eggan KC, Maniatis T, Taylor JP (2014) Axonal transport of TDP-43 mRNA granules is impaired by ALS-causing mutations. Neuron 81:536–543. https://doi.org/10.1016/j.neuron.2013.12.018 CrossRefPubMedPubMedCentralGoogle Scholar
- 3.Arai T, Hasegawa M, Akiyama H, Ikeda K, Nonaka T, Mori H, Mann D, Tsuchiya K, Yoshida M, Hashizume Y, Oda T (2006) TDP-43 is a component of ubiquitin-positive tau-negative inclusions in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Biochem Biophys Res Commun 351:602–611. https://doi.org/10.1016/j.bbrc.2006.10.093 CrossRefPubMedGoogle Scholar
- 7.Boeynaems S, Bogaert E, Kovacs D, Konijnenberg A, Timmerman E, Volkov A, Guharoy M, De Decker M, Jaspers T, Ryan VH, Janke AM, Baatsen P, Vercruysse T, Kolaitis R-M, Daelemans D, Taylor JP, Kedersha N, Anderson P, Impens F, Sobott F, Schymkowitz J, Rousseau F, Fawzi NL, Robberecht W, Van Damme P, Tompa P, Van Den Bosch L (2017) Phase separation of C9orf72 dipeptide repeats perturbs stress granule dynamics. Mol Cell 65:1044–1055. https://doi.org/10.1016/j.molcel.2017.02.013 CrossRefPubMedPubMedCentralGoogle Scholar
- 10.Braak H, Ludolph A, Thal DR, Del Tredici K (2010) Amyotrophic lateral sclerosis: dash-like accumulation of phosphorylated TDP-43 in somatodendritic and axonal compartments of somatomotor neurons of the lower brainstem and spinal cord. Acta Neuropathol 120:67–74. https://doi.org/10.1007/s00401-010-0683-0 CrossRefPubMedGoogle Scholar
- 11.Brettschneider J, Del Tredici K, Toledo JB, Robinson JL, Irwin DJ, Grossman M, Suh E, Van Deerlin VM, Wood EM, Baek Y, Kwong L, Lee EB, Elman L, McCluskey L, Fang L, Feldengut S, Ludolph AC, Lee VM-Y, Braak H, Trojanowski JQ (2013) Stages of pTDP-43 pathology in amyotrophic lateral sclerosis. Ann Neurol 74:20–38. https://doi.org/10.1002/ana.23937 CrossRefPubMedPubMedCentralGoogle Scholar
- 12.Budini M, Buratti E, Stuani C, Guarnaccia C, Romano V, De Conti L, Baralle FE (2012) Cellular model of TAR DNA-binding protein 43 (TDP-43) aggregation based on its C-terminal Gln/Asn-rich region. J Biol Chem 287:7512–7525. https://doi.org/10.1074/jbc.M111.288720 CrossRefPubMedPubMedCentralGoogle Scholar
- 15.Davidson Y, Kelley T, Mackenzie IRA, Pickering-Brown S, Du Plessis D, Neary D, Snowden JS, Mann DMA (2007) Ubiquitinated pathological lesions in frontotemporal lobar degeneration contain the TAR DNA-binding protein, TDP-43. Acta Neuropathol 113:521–533. https://doi.org/10.1007/s00401-006-0189-y CrossRefPubMedGoogle Scholar
- 17.Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SSL, Stoddart JJ, Nicholson G, Chung R, Cole NJ (2017) A Tol2 gateway-compatible toolbox for the study of the nervous system and neurodegenerative disease. Zebrafish 14:69–72. https://doi.org/10.1089/zeb.2016.1321 CrossRefPubMedGoogle Scholar
- 21.Feiler MS, Strobel B, Freischmidt A, Helferich AM, Kappel J, Brewer BM, Li D, Thal DR, Walther P, Ludolph AC, Danzer KM, Weishaupt JH (2015) TDP-43 is intercellularly transmitted across axon terminals. J Cell Biol 211:897–911. https://doi.org/10.1083/jcb.201504057 CrossRefPubMedPubMedCentralGoogle Scholar
- 23.Giordana MT, Piccinini M, Grifoni S, De Marco G, Vercellino M, Magistrello M, Pellerino A, Buccinnà B, Lupino E, Rinaudo MT (2010) TDP-43 redistribution is an early event in sporadic amyotrophic lateral sclerosis. Brain Pathol 20:351–360. https://doi.org/10.1111/j.1750-3639.2009.00284.x CrossRefPubMedGoogle Scholar
- 24.Graeber MB, Moran LB (2002) Mechanisms of cell death in neurodegenerative diseases: fashion, fiction, and facts. Brain Pathol 12:385–390. https://doi.org/10.1111/j.1750-3639.2002.tb00452.x CrossRefPubMedGoogle Scholar
- 27.Johnson BS, Snead D, Lee JJ, McCaffery JM, Shorter J, Gitler AD (2009) TDP-43 is intrinsically aggregation-prone, and amyotrophic lateral sclerosis-linked mutations accelerate aggregation and increase toxicity. J Biol Chem 284:20329–20339. https://doi.org/10.1074/jbc.M109.010264 CrossRefPubMedPubMedCentralGoogle Scholar
- 29.Ke YD, van Hummel A, Stevens CH, Gladbach A, Ippati S, Bi M, Lee WS, Krüger S, van der Hoven J, Volkerling A, Bongers A, Halliday G, Haass NK, Kiernan M, Delerue F, Ittner LM (2015) Short-term suppression of A315T mutant human TDP-43 expression improves functional deficits in a novel inducible transgenic mouse model of FTLD-TDP and ALS. Acta Neuropathol 130:661–678. https://doi.org/10.1007/s00401-015-1486-0 CrossRefPubMedGoogle Scholar
- 30.Kim H-J, Raphael AR, LaDow ES, McGurk L, Weber RA, Trojanowski JQ, Lee VM-Y, Finkbeiner S, Gitler AD, Bonini NM (2013) Therapeutic modulation of eIF2α phosphorylation rescues TDP-43 toxicity in amyotrophic lateral sclerosis disease models. Nat Genet 46:152–160. https://doi.org/10.1038/ng.2853 CrossRefPubMedPubMedCentralGoogle Scholar
- 34.Lagier-Tourenne C, Polymenidou M, Hutt KR, Vu AQ, Baughn M, Huelga SC, Clutario KM, Ling S-C, Liang TY, Mazur C, Wancewicz E, Kim AS, Watt A, Freier S, Hicks GG, Donohue JP, Shiue L, Bennett F, Ravits J, Cleveland DW, Yeo GW (2012) Divergent roles of ALS-linked proteins FUS/TLS and TDP-43 intersect in processing long pre-mRNAs. Nat Neurosci 15:1488–1497. https://doi.org/10.1038/nn.3230 CrossRefPubMedPubMedCentralGoogle Scholar
- 37.Love S (2015) Spinal muscular atrophy (SMA): neuronal swelling. In: Love S, Perry A, Ironside J, Budka H (eds) Greenfield’s neuropathology, 9th edn. CRC Press, Boca Raton, FL, p 838Google Scholar
- 38.Lowe J, Mirra SS, Hyman BT, Dickson DW (2008) Accumulation of intracellular proteins as inclusion bodies: proteinopathies. In: Love S, Louis DN, Ellison DW (eds) Greenfield’s neuropathology, 8th edn. CRC Press, Boca Raton, pp 1031–1032Google Scholar
- 39.Moisse K, Volkening K, Leystra-Lantz C, Welch I, Hill T, Strong MJ (2009) Divergent patterns of cytosolic TDP-43 and neuronal progranulin expression following axotomy: implications for TDP-43 in the physiological response to neuronal injury. Brain Res 1249:202–211. https://doi.org/10.1016/j.brainres.2008.10.021 CrossRefPubMedGoogle Scholar
- 43.Neumann M, Sampathu DM, Kwong LK, Truax AC, Micsenyi MC, Chou TT, Bruce J, Schuck T, Grossman M, Clark CM, McCluskey LF, Miller BL, Masliah E, Mackenzie IR, Feldman H, Feiden W, Kretzschamer HA, Trojanowski JQ, Lee VM-Y (2006) Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science 314:130–133. https://doi.org/10.1126/science.1134108 CrossRefPubMedGoogle Scholar
- 45.Onozato T, Nakahara A, Suzuki-Kouyama E, Hineno A, Yasude T, Nakamura T, Yahikozawa H, Watanabe M, Kayanuma K, Makishita H, Ohara S, Hashimoto T, Higuchi K, Sakai T, Asano K, Hashimoto T, Kanno H, Nakayama J, Oyanagi K (2016) Axonal TDP-43 aggregates in sporadic amyotrophic lateral sclerosis. Neuropathol Appl Neurobiol 42:561–572. https://doi.org/10.1111/nan.12310 CrossRefPubMedGoogle Scholar
- 46.Paolicelli RC, Jawaid A, Henstridge CM, Valeri A, Merlini M, Robinson JL, Lee EB, Rose J, Appel S, Lee VM-Y, Trojanowski JQ, Spires-Jones T, Schulz PE, Rajendran L (2017) TDP-43 depletion in microglia promotes amyloid clearance but also induces synapse loss. Neuron 95:1–12. https://doi.org/10.1016/j.neuron.2017.05.037 CrossRefGoogle Scholar
- 49.Prajsnar TK, Cunliffe VT, Foster SJ, Renshaw SA (2008) A novel vertebrate model of Staphylococcus aureus infection reveals phagocyte-dependent resistance of zebrafish to non-host specialized pathogens. Cell Microbiol 10:2312–2325. https://doi.org/10.1111/j.1462-5822.2008.01213.x CrossRefPubMedGoogle Scholar
- 52.Rüb U, Vonsattel JPG, Heinsen H, Korf H-W (2015) Pathological nerve cell alterations in Huntington’s disease (HD) and their possible role for the demise of nerve cells. In: Rüb U, Vonsattel JPG, Heinsen H, Korf H-W (eds) The neuropathology of Huntington’s disease: classical findings, recent developments and correlation to functional neuroanatomy. Springer International Publishing, Cham, pp 119–123CrossRefGoogle Scholar
- 54.Sato T, Takeuchi S, Saito A, Ding W, Bamba H, Matsuura H, Hisa Y, Tooyama I, Urushitani M (2009) Axonal ligation induces transient redistribution of TDP-43 in brainstem motor neurons. Neuroscience 164:1565–1578. https://doi.org/10.1016/j.neuroscience.2009.09.050 CrossRefPubMedGoogle Scholar
- 55.Sephton CF, Cenik C, Kucukural A, Dammer EB, Cenik B, Han Y, Dewey CM, Roth FP, Herz J, Peng J, Moore MJ, Yu G (2011) Identification of neuronal RNA targets of TDP-43-containing ribonucleoprotein complexes. J Biol Chem 286:1204–1215. https://doi.org/10.1074/jbc.M110.190884 CrossRefPubMedGoogle Scholar
- 59.Spiller KJ, Restrepo CR, Khan T, Dominique MA, Fang TC, Canter RG, Roberts CJ, Miller KR, Ransohoff RM, Trojanowski JQ, Lee VM-Y (2018) Microglia-mediated recovery from ALS-relevant motor neuron degeneration in a mouse model of TDP-43 proteinopathy. Nat Neurosci 21:329. https://doi.org/10.1038/s41593-018-0083-7 CrossRefPubMedPubMedCentralGoogle Scholar
- 61.Sumi H, Kato S, Mochimaru Y, Fujimura H, Etoh M, Sakoda S (2009) Nuclear TAR DNA binding protein 43 expression in spinal cord neurons correlates with the clinical course in amyotrophic lateral sclerosis. J Neuropathol Exp Neurol 68:37–47. https://doi.org/10.1097/NEN.0b013e3181919cb5 CrossRefPubMedGoogle Scholar
- 63.Walker AK, Spiller KJ, Ge G, Zheng A, Xu Y, Zhou M, Tripathy K, Kwong LK, Trojanowski JQ, Lee VM-Y (2015) Functional recovery in new mouse models of ALS/FTLD after clearance of pathological cytoplasmic TDP-43. Acta Neuropathol 130:643–660. https://doi.org/10.1007/s00401-015-1460-x CrossRefPubMedPubMedCentralGoogle Scholar
- 65.Westerfield M (2000) The zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio). University of Oregon Press, EugeneGoogle Scholar
- 66.Winton MJ, Igaz LM, Wong MM, Kwong LK, Trojanowski JQ, Lee VM-Y (2008) Disturbance of nuclear and cytoplasmic TAR DNA-binding protein (TDP-43) induces disease-like redistribution, sequestration, and aggregate formation. J Biol Chem 283:13302–13309. https://doi.org/10.1074/jbc.M800342200 CrossRefPubMedPubMedCentralGoogle Scholar
- 71.Zhu L, Xu M, Yang M, Yang Y, Li Y, Deng J, Ruan L, Liu J, Du S, Liu X, Feng W, Fushimi K, Bigio EH, Mesulam M, Wang C, Wu JY (2014) An ALS-mutant TDP-43 neurotoxic peptide adopts an anti-parallel b-structure and induces TDP-43 redistribution. Hum Mol Genet 23:6863–6877. https://doi.org/10.1093/hmg/ddu409 CrossRefPubMedPubMedCentralGoogle Scholar
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