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Cloning a peanut resveratrol synthase gene and its expression in purple sweet potato

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Abstract

A resveratrol synthase gene was cloned from the peanut plant (Arachis hypogaea) by RT-PCR and was transformed into purple sweet potato (Ipomoea batatas) by Agrobacterium-mediated transformation. Stem sections were infected with bacterial solution of OD600 = 0.4 for 20 min and then cocultured for 2 days. Infected explants were cultured on MS media containing 50 mg/l kanamycin, 0.02 mg/l NAA and 1 mg/l 6-BA for bud induction or containing 75 mg/l kanamycin, 1.0 mg/l NAA and 0.1 mg/l 6-BA for root formation. The bud and root induction rates were 37.5 and 25.0%, respectively. 105 regenerated plants were obtained, with 11 positive plants by PCR and Southern blotting analyses. A high level of resveratrol glucoside (340 μg/g dry weight), but no resveratrol, was detected in the transformed plants by HPLC. This study also provides a stable genetic transformation and plant regeneration method for metabolic modification of purple sweet potato.

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Abbreviations

6-BA:

6-Benzylaminopurine

CaMV:

Cauliflower mosaic virus

CHS:

Chalcone synthase

CK:

Control

CTAB:

Cetyl trimethylammonium bromide

GUS:

β-Glucuronidase

IPTG:

Isopropyl-β-d-thiogalactopyranoside

KT:

Kinetin

MS:

Murashige and Skoog medium

NAA:

2,4-Dichlorophenoxyacetic acid

RS:

Resveratrol synthase

UV:

Ultra violet

X-gluc:

5-Bromo-4-chloro-3-indolyl-β-d-glucuronide

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Acknowledgments

This work was supported by the Guangdong Provincial Science and Technology Programs (2006B20101005, 2011B020310008), Science and Technology Supporting Programs of Guangzhou Municipal Government (2008Z1-E591) and Panyu District Science, Technology Programs of Guangzhou City (2009-T-17-1) and Guangdong Natural Science Fund (program number 10151063101000002, S2011010003368), with all funds to H.-H. Li.

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  1. Li-Ping Pan

    Present address: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, School of Basic Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dongdan 3 Tiao, Beijing, 100005, China

Authors and Affiliations

  1. Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, 510631, China

    Li-Ping Pan, Si-Li Yu, Chang-Jian Chen, Hui Li, Yi-Liang Wu & Hai-Hang Li

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  1. Li-Ping Pan
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Correspondence to Hai-Hang Li.

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Communicated by H. Jones.

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Pan, LP., Yu, SL., Chen, CJ. et al. Cloning a peanut resveratrol synthase gene and its expression in purple sweet potato. Plant Cell Rep 31, 121–131 (2012). https://doi.org/10.1007/s00299-011-1145-4

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  • DOI: https://doi.org/10.1007/s00299-011-1145-4

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