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Change of microbial community structure and functional gene abundance in nonylphenol-degrading sediment

  • Environmental biotechnology
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Abstract

Biodegradation by autochthonous microbial community is an important way to clean up nonylphenol (NP) from contaminated river sediment. Knowledge of sediment microbial community can aid in our understanding of biological processes related to NP degradation. However, the change in sediment microbial community associated with NP biodegradation remains unclear. The present study investigated the shift in bacterial community structure and NP-degrading gene abundance in response to NP attenuation in river sediment. Sediment microcosms with different levels of 4-NP (0, 100, or 300 μg/g) were constructed. A nearly complete attenuation of NP occurred in the microcosm with 100 μg/g NP after 9 days’ incubation, while a residual NP rate of 8.1 % was observed in the microcosm with 300 μg/g NP after 22 days’ incubation. Illumina MiSeq sequencing analysis indicated that Gammaproteobacteria, Alphaproteobacteria, and Bacteroidetes predominated in NP-degrading river sediment. Sediment bacterial community structure varied significantly during NP biodegradation and subsequent incubation, which was affected by the level of added NP. The n-alkane biodegradation (alkB) gene abundance showed a significant variation in each NP-amended microcosm (100 or 300 μg/g), while a significant increase in the single component monooxygenase (sMO) gene abundance only occurred in the microcosm spiked with 300 μg/g NP. This study can provide some new insights toward NP-degrading microbial ecology in the environment.

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Acknowledgments

This work was financially supported by a special fund of the State Key Joint Laboratory of Environment Simulation and Pollution Control (No. 14Y02ESPCP and No. 13K07ESPCT).

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Authors and Affiliations

  1. State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China

    Zhao Wang, Yuyin Yang & Shuguang Xie

  2. South China Institute of Environmental Sciences, Ministry of Environment Protection (MEP), Guangzhou, 510655, China

    Tao He

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  1. Zhao Wang
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  2. Yuyin Yang
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  4. Shuguang Xie
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Correspondence to Shuguang Xie.

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Zhao Wang and Yuyin Yang contributed equally to this study.

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Wang, Z., Yang, Y., He, T. et al. Change of microbial community structure and functional gene abundance in nonylphenol-degrading sediment. Appl Microbiol Biotechnol 99, 3259–3268 (2015). https://doi.org/10.1007/s00253-014-6222-5

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  • DOI: https://doi.org/10.1007/s00253-014-6222-5

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