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Variation of nonylphenol-degrading gene abundance and bacterial community structure in bioaugmented sediment microcosm

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Abstract

Nonylphenol (NP) can accumulate in river sediment. Bioaugmentation is an attractive option to dissipate heavy NP pollution in river sediment. In this study, two NP degraders were isolated from crude oil-polluted soil and river sediment. Microcosms were constructed to test their ability to degrade NP in river sediment. The shift in the proportion of NP-degrading genes and bacterial community structure in sediment microcosms were characterized using quantitative PCR assay and terminal restriction fragment length polymorphism analysis, respectively. Phylogenetic analysis indicated that the soil isolate belonged to genus Stenotrophomonas, while the sediment isolate was a Sphingobium species. Both of them could almost completely clean up a high level of NP in river sediment (150 mg/kg NP) in 10 or 14 days after inoculation. An increase in the proportion of alkB and sMO genes was observed in sediment microcosms inoculated with Stenotrophomonas strain Y1 and Sphingobium strain Y2, respectively. Moreover, bioaugmentation using Sphingobium strain Y2 could have a strong impact on sediment bacterial community structure, while inoculation of Stenotrophomonas strain Y1 illustrated a weak impact. This study can provide some new insights towards NP biodegradation and bioremediation.

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Acknowledgments

This work was financially supported by a special fund from State Key Joint Laboratory of Environment Simulation and Pollution Control (No. 14Y02ESPCP) and (No. 13K07ESPCT).

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Authors and Affiliations

  1. State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China

    Zhao Wang, Yuyin Yang, Yu Dai & Shuguang Xie

  2. Department of Environmental Science, Rutgers University, New Brunswick, NJ, 08901, USA

    Weimin Sun

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  1. Zhao Wang
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  2. Yuyin Yang
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  3. Weimin Sun
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  4. Yu Dai
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  5. Shuguang Xie
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Correspondence to Shuguang Xie.

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Responsible editor: Philippe Garrigues

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Wang, Z., Yang, Y., Sun, W. et al. Variation of nonylphenol-degrading gene abundance and bacterial community structure in bioaugmented sediment microcosm. Environ Sci Pollut Res 22, 2342–2349 (2015). https://doi.org/10.1007/s11356-014-3625-x

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  • DOI: https://doi.org/10.1007/s11356-014-3625-x

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