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The Two-Operon-Coded ABC Transporter Complex FpvWXYZCDEF is Required for Pseudomonas aeruginosa Growth and Virulence Under Iron-Limiting Conditions

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Abstract

Iron is essential for all organisms. Bacteria have devolved sophisticated systems to maintain intracellular iron homeostasis. FpvCDEF(PA2407-2410) has been reported as an ABC transporter involved in pyoverdine-Fe uptake which does not affect growth under iron-limiting condition, when it is deleted in PAO1. In this study, we proved that fpvCDEF and fpvWXYZ(PA2403-2406) constituted an ABC transporter complex containing two operons: fpvWXYZCDE and fpvF. The operon fpvWXYZCDE was regulated by iron negatively and the single gene operon fpvF was constitutively expressed. Inactivation of any one of the components, fpvW, fpvC, fpvD, fpvE, and fpvF, led to increased expression of fpvWXYZCDE suggesting that each component of fpvWXYZCDEF could be involved in iron uptake. The ABC transporter complex encoded by fpvWXYZCDEF plays important roles in growth, oxidative stress resistance, and virulence, since the deletion of fpvWXYZCDEF resulted in defective growth, increased sensitivity to H2O2, and decreased virulence compared with PAO1(ΔfpvCDEF) and the wild type PAO1 under iron-limiting condition.

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Acknowledgements

This work was supported by NSFC (81171620, 31570131), NSERC Canada (No. 402943-2011 RGPIN), PCSIRT (IRT 15R55), and Science and Technology Overall Innovation Project of Shaanxi Province China (2016KTCQ03-18).

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  1. Lang Gao and Zisheng Guo have equally contributed to this work.

Authors and Affiliations

  1. Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi’an, 710069, China

    Lang Gao, Zisheng Guo, Yue Wang, Yulu Wang, Kerong Wang, Bo Li & Lixin Shen

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  1. Lang Gao
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  2. Zisheng Guo
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Correspondence to Bo Li or Lixin Shen.

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Gao, L., Guo, Z., Wang, Y. et al. The Two-Operon-Coded ABC Transporter Complex FpvWXYZCDEF is Required for Pseudomonas aeruginosa Growth and Virulence Under Iron-Limiting Conditions. J Membrane Biol 251, 91–104 (2018). https://doi.org/10.1007/s00232-017-9979-5

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