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Determination of lysine content based on an in situ pretreatment and headspace gas chromatographic measurement technique

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Abstract

This work reports on a simple method for the determination of lysine content by an in situ sample pretreatment and headspace gas chromatographic measurement (HS-GC) technique, based on carbon dioxide (CO2) formation from the pretreatment reaction (between lysine and ninhydrin solution) in a closed vial. It was observed that complete lysine conversion to CO2 could be achieved within 60 min at 60 °C in a phosphate buffer medium (pH = 4.0), with a minimum molar ratio of ninhydrin/lysine of 16. The results showed that the method had a good precision (RSD < 5.23%) and accuracy (within 6.80%), compared to the results measured by a reference method (ninhydrin spectroscopic method). Due to the feature of in situ sample pretreatment and headspace measurement, the present method becomes very simple and particularly suitable to be used for batch sample analysis in lysine-related research and applications.

The flow path of the reaction and HS-GC measurement for the lysine analysis

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Funding

The authors acknowledge the financial support from the National Major Research Project (Project No. 2017YFB0307900). This work is also partly supported by Science and Technology Planning Project of Guangdong Province (Project No. 2017B090901064).

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Authors and Affiliations

  1. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, 381 Wushan Road, Tianhe District, Guangzhou, Guangdong, 510640, China

    Xiao-fang Wan, Bao-lian Liu, Ning Yan, Xin-Sheng Chai, You-ming Li & Guang-xue Chen

  2. School of Materials Science and Engineering, South China University of Technology, 381 Wushan Road, Tianhe District, Guangzhou, Guangdong, 510640, China

    Teng Yu

Authors
  1. Xiao-fang Wan
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  2. Bao-lian Liu
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  3. Teng Yu
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  4. Ning Yan
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  5. Xin-Sheng Chai
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  6. You-ming Li
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  7. Guang-xue Chen
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Correspondence to Xin-Sheng Chai.

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Wan, Xf., Liu, Bl., Yu, T. et al. Determination of lysine content based on an in situ pretreatment and headspace gas chromatographic measurement technique. Anal Bioanal Chem 410, 3111–3117 (2018). https://doi.org/10.1007/s00216-018-0998-6

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  • DOI: https://doi.org/10.1007/s00216-018-0998-6

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