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Marinococcus salis sp., nov., a moderately halophilic bacterium isolated from a salt marsh

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Abstract

A novel Gram-stain-positive, coccoid-shaped, facultative anaerobic, motile and halophilic bacterium strain 5MT was isolated from Surajbari in India. Based on the 16S rRNA gene sequence analysis, it was identified as belonging to the genus Marinococcus and was most closely related to Marinococcus luteus KCTC 13214T (99.3 %, sequence similarity), Marinococcus halotolerans KCTC 19045T (99.0 %), Marinococcus halophilus LMG 17439T (98.8 %) and Marinococcus tarijensis LMG 26930T (98.7 %). However, the DNA–DNA relatedness of strain 5MT with M. luteus KCTC 13214T, M. halotolerans KCTC 19045T, M. halophilus LMG 17439T and M. tarijensis LMG 26930T was 42.6 ± 0.8, 48.6 ± 0.8, 40.9 ± 0.8 and 39.8 ± 0.9 %, respectively. Strain 5MT grows optimally at 5 % (w/v) NaCl, pH 7.5–8.5 and 37 °C. The cell-wall peptidoglycan of strain 5MT contains meso-diaminopimelic acid. Polar lipids of the strain 5MT include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a phospholipid and two unknown lipids. The predominant isoprenoid quinone was MK-7. DNA G+C content was 48.9 mol%, and anteiso-C15:0 (40.9 %) was the predominant fatty acid. The results of phylogenetic, biochemical tests and chemotaxonomic allowed a clear differentiation of strain 5MT from all of its nearest phylogenetic neighbours, which represents a novel member of the genus Marinococcus, for which the name Marinococcus salis sp., nov., is proposed. The type strain is 5MT (=KCTC 33743T = LMG 29101T = CGMCC 1.15385T).

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Acknowledgments

S.V.R. and M.T. thank Department of Science and Technology, Government of India, for providing FTSYS Project Grants (SB/FT/LS-115/2012 and SB/FT/LS-320/2012, respectively). M.F. also thanks DST for PA fellowship.

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Authors and Affiliations

  1. Microbial Ecology Laboratory, Department of Biochemistry, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda, Telangana, 508254, India

    Sultanpuram Vishnuvardhan Reddy, Mothe Thirumala & Mohammed Farooq

  2. Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad, Telangana, 500085, India

    Chintalapati Sasikala

  3. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, Telangana, 500046, India

    Chintalapati Venkata Ramana

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  1. Sultanpuram Vishnuvardhan Reddy
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  2. Mothe Thirumala
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  4. Chintalapati Sasikala
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  5. Chintalapati Venkata Ramana
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Corresponding author

Correspondence to Sultanpuram Vishnuvardhan Reddy.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequences of strain 5MT is LN879357.

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Supplementary Fig. 1

Two-dimensional thin-layer chromatogram of polar lipid extracts of strain 5MT and related Marinococcus species. The first direction was developed in CHCl3:CH3OH:H2O (75:32:4 by volume) and the second in CHCl3:CH3OH:CH3COOH:H2O (86:16:15:4 by volume). PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PL1, unknown phospholipid; and L1-7, unknown lipids (PDF 437 kb)

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Vishnuvardhan Reddy, S., Thirumala, M., Farooq, M. et al. Marinococcus salis sp., nov., a moderately halophilic bacterium isolated from a salt marsh. Arch Microbiol 198, 1013–1018 (2016). https://doi.org/10.1007/s00203-016-1263-z

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