Abstract
Key message
The role of miRNAs during viral pathogenesis is poorly understood in plants. Here, we demonstrate a miRNA/target module that acts as a susceptibility factor during ToLCNDV infection.
Abstract
Tomato leaf curl New Delhi virus (ToLCNDV) is a devastating pathogen that causes huge crop loss. It is spreading to new geographical locations at a very rapid rate-raising serious concerns. Evolution of insecticidal resistance in Bemisia tabaci which acts as the carrier for ToLCNDV has made insect control very difficult in the recent years. Thus, it is important that the host molecular mechanisms associated with ToLCNDV resistance/susceptibility are investigated to develop management strategies. In our study, we have identified that sly-miR166/SlyHB module acts as a susceptibility factor to ToLCNDV in Solanum lycopersicum. Sly-miR166 is differentially regulated upon ToLCNDV infection in two contrasting tomato cultivars; H-88-78-1 (tolerant to ToLCNDV) and Punjab Chhuhara (susceptible to ToLCNDV). Expression analysis of predicted sly-miR166 targets revealed that the expression of SlyHB is negatively correlated with its corresponding miRNA. Virus-induced gene silencing of SlyHB in the susceptible tomato cultivar resulted in the decrease in disease severity suggesting that SlyHB is a negative regulator of plant defence. In summary, our study highlights a miRNA/target module that acts as a susceptibility factor during ToLCNDV infection. To the best of our knowledge, this is the first report that highlights the role of sly-miR166/SlyHB module in ToLCNDV pathogenesis.
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Acknowledgements
This work was supported by projects from Ministry of Science and Technology, Govt. of India [National Institute of Plant Genome Research core grant and JC Bose Fellowship JCB/2018/000001] to M.P. Research fellowship from Council of Scientific and Industrial Research, Govt. of India to A.P. We thank Prof. Savithramma Dinesh-Kumar, Plant Biology Department, University of California, Davis, USA for providing TRV-based VIGS vectors. We thank DBT-eLibrary Consortium (DeLCON) for providing access to e-resources.
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MP, NS and AP planned the research. AP performed the experiments. NS and OC assisted AP with the experiments. AP and NS analysed the data. AP wrote the paper.
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Communicated by Richard G. F. Visser.
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Prasad, A., Sharma, N., Chirom, O. et al. The sly-miR166-SlyHB module acts as a susceptibility factor during ToLCNDV infection. Theor Appl Genet 135, 233–242 (2022). https://doi.org/10.1007/s00122-021-03962-4
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DOI: https://doi.org/10.1007/s00122-021-03962-4