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Paraquat resistance in a Lolium rigidum population is governed by one major nuclear gene

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Abstract

Paraquat resistance in an annual ryegrass (Lolium rigidum Gaud.) population (AFLR1) has been attributed to reduced paraquat translocation. Genetic inheritance of paraquat resistance in this population was investigated in the present study. The paraquat dose response of progeny from 8 F1 families was more similar to that of the resistant than the susceptible parent, while the equivalent data for a further three families were intermediate compared to those of the parental populations. No significant differences in dose response were observed between reciprocal crosses of specific F1 families. These results suggest that paraquat resistance in AFLR1 is inherited as a dominant or partially dominant nuclear-encoded trait. Pseudo-F2 (ψ-F2) generation seedlings were treated with multiple dose rates sufficient to control the susceptible parental population, and observed segregation ratios in all instances conformed to a 3:1 (resistant:susceptible) segregation ratio, and this ratio was further confirmed by individual phenotyping of cloned plant genotypes. A single major nuclear gene is hence apparently responsible for evolved paraquat resistance in AFLR1.

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Acknowledgments

The Western Australian Herbicide Research Initiative (WAHRI) is funded by the Grains Research and Development Corporation of Australia (GRDC). This research is partially funded by the Australian Research Council through an ARC-Linkage Project (LP0669035). The authors wish to thank Dr. Noel Cogan for fruitful discussion.

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Authors and Affiliations

  1. Western Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, Crawley, WA, 6009, Australia

    Qin Yu, Heping Han, Linh Nguyen & Stephen B. Powles

  2. Biosciences Research Division, Department of Primary Industries, Victorian AgriBiosciences Centre, La Trobe University Research and Development Park, 1 Park Drive, Bundoora, VIC, Australia

    Linh Nguyen & John W. Forster

Authors
  1. Qin Yu
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  2. Heping Han
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  3. Linh Nguyen
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  4. John W. Forster
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  5. Stephen B. Powles
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Correspondence to Qin Yu.

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Communicated by M. Kearsey.

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Yu, Q., Han, H., Nguyen, L. et al. Paraquat resistance in a Lolium rigidum population is governed by one major nuclear gene. Theor Appl Genet 118, 1601–1608 (2009). https://doi.org/10.1007/s00122-009-1008-3

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  • DOI: https://doi.org/10.1007/s00122-009-1008-3

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