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Recent advances in the development of new transgenic animal technology

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Abstract

Transgenic animal technology is one of the fastest growing biotechnology areas. It is used to integrate exogenous genes into the animal genome by genetic engineering technology so that these genes can be inherited and expressed by offspring. The transgenic efficiency and precise control of gene expression are the key limiting factors in the production of transgenic animals. A variety of transgenic technologies are available. Each has its own advantages and disadvantages and needs further study because of unresolved technical and safety issues. Further studies will allow transgenic technology to explore gene function, animal genetic improvement, bioreactors, animal disease models, and organ transplantation. This article reviews the recently developed animal transgenic technologies, including the germ line stem cell-mediated method to improve efficiency, gene targeting to improve accuracy, RNA interference-mediated gene silencing technology, zinc-finger nuclease gene targeting technology and induced pluripotent stem cell technology. These new transgenic techniques can provide a better platform to develop transgenic animals for breeding new animal varieties and promote the development of medical sciences, livestock production, and other fields.

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Acknowledgments

This work was supported by a grant from The Major Science and Technology Project of New Variety Breeding of Genetically Modified Organisms (Nos. 2009ZX08008-004B and 2008ZX08008-003), the National High Technology Research Development Program of China (863 Program No. 2008AA10Z140), the National Natural Science Foundation of China (No. 30571339) and the Innovation Research Foundation of CAAS (No. 2004-CAAS-1).

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  1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Xiangyang Miao

  2. Harvard Medical School, Boston, MA, 02114, USA

    Xiangyang Miao

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Correspondence to Xiangyang Miao.

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Miao, X. Recent advances in the development of new transgenic animal technology. Cell. Mol. Life Sci. 70, 815–828 (2013). https://doi.org/10.1007/s00018-012-1081-7

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  • DOI: https://doi.org/10.1007/s00018-012-1081-7

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