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Generating Conditional Knockout Mice

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Transgenic Mouse Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 693))

Abstract

Gene targeting in ES cells is extensively used to generate designed mouse mutants and to study gene function in vivo. Knockout mice that harbor a null allele in their germline provide appropriate genetic models of inherited diseases and often exhibit embryonic or early postnatal lethality. To study gene function in adult mice and in selected cell types, a refined strategy for conditional gene inactivation has been developed that relies on the DNA recombinase Cre and its recognition (loxP) sites. For conditional mutagenesis, a target gene is modified by the insertion of two loxP sites that enable to excise the flanked (floxed) gene segment through Cre-mediated recombination. Conditional mutant mice are obtained by crossing the floxed strain with a Cre transgenic line such that the target gene becomes inactivated in vivo within the expression domain of Cre. A large collection of Cre transgenic lines has been generated over time and can be used in a combinatorial manner to achieve gene inactivation in many different cell types. A growing number of CreERT2 transgenic mice further allows for inducible inactivation of floxed alleles in adult mice upon administration of tamoxifen. This chapter covers the design and construction of loxP flanked alleles and refers to the vectors, ES cells, and mice generated by the European conditional mouse mutagenesis (EUCOMM) project. We further describe the design and use of Cre and CreERT2 transgenic mice and a convenient breeding strategy to raise conditional mutants and controls for phenotype analysis.

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Authors and Affiliations

  1. Mount Sinai School of Medicine, New York, NY, USA

    Roland H. Friedel

  2. Institute of Developmental Genetics, Helmholtz Center Munich – German Research Center for Environmental Health, Neuherberg/Munich, Germany

    Wolfgang Wurst, Benedikt Wefers & Ralf Kühn

  3. Molecular Neurogenetics, Max-Planck-Institute of Psychiatry, Munich, Germany

    Wolfgang Wurst

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  1. Roland H. Friedel
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  3. Benedikt Wefers
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  4. Ralf Kühn
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Editor information

Editors and Affiliations

  1. Dept. Pathology & Medical Biology, Medical Biology Section, University Medical Center Groningen, Antonius Deusinglaan 1, Groningen, 9713 AV, Netherlands

    Marten H. Hofker

  2. Mayo Clinic, First Street SW. 200, Rochester, 55905, Minnesota, USA

    Jan van Deursen

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© 2011 Springer Science+Business Media, LLC

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Friedel, R.H., Wurst, W., Wefers, B., Kühn, R. (2011). Generating Conditional Knockout Mice. In: Hofker, M., van Deursen, J. (eds) Transgenic Mouse Methods and Protocols. Methods in Molecular Biology, vol 693. Humana Press. https://doi.org/10.1007/978-1-60761-974-1_12

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  • DOI: https://doi.org/10.1007/978-1-60761-974-1_12

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-973-4

  • Online ISBN: 978-1-60761-974-1

  • eBook Packages: Springer Protocols

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