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Complete Amino Acid Sequence of a Subunit from Rapeseed Protein

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Abstract

A subunit of molecular weight 18300 has been separated and isolated from seeds of Brassica campestris L. This subunit was cleaved by using cyanogen bromide, trypsin, Staphylococcus aureus V8 protease and chymotrypsin; the fragments obtained from enzymatlc and chemical cleavages were separated and isolated by polyacrylamide gel electrophoresis and gel filtration. The amino acid analyses were carried out. The complete amino acid sequence of the subunit containing 172 amino acid residues has been established by manual Edman method.

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Authors and Affiliations

  1. Department of Chemistry, University of Roorkee, Roorkee, 247 667, India

    Ravi Bhushan & Anil K. Tyagi

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  1. Ravi Bhushan
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  2. Anil K. Tyagi
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Bhushan, R., Tyagi, A.K. Complete Amino Acid Sequence of a Subunit from Rapeseed Protein. J. Plant Biochem. Biotechnol. 7, 13–21 (1998). https://doi.org/10.1007/BF03263027

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  • DOI: https://doi.org/10.1007/BF03263027

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