Abstract
Morphological, developmental and antimetabolite-resistant mutants ofT. languinosus were characterized and used for screening with the aim to develop constitutive α-amylase-hyperproducing strains. The protoplast fusion of two spontaneous mutants ofT. lanuginosus, characterized as asporulating and resistant to 2-deoxy-d-glucose (2DG), resulted in sporulating, 2DG sensitive heterokaryotic fusants. A recombinant haploid strain F64fB developed there from produced α-amylase constitutively in glucose-containing medium. Constitutive α-amylase-hyperproducing mutant (III8) obtained after cyclic mutagenesis and screening yielded ≈20 fold more α-amylase in a glycerol-containing medium than the wild strain.
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This work was supported by research grant (BT/13/12/94-PID) of theDepartment of Biotechnology, Government of India.
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Chadha, B.S., Rubinder, K. & Saini, H.S. Constitutive α-amylase producing mutant and recombinant haploid strains of thermophilic fungusThermomyces lanuginosus . Folia Microbiol 50, 133–140 (2005). https://doi.org/10.1007/BF02931462
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DOI: https://doi.org/10.1007/BF02931462