Skip to main content
SpringerLink
Log in

Difference in resistance of subunits A and B ofVibrio cholerae toxin (choleragen) to treatment with pronase

  • Specialia
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Disc electrophoresis in sodium dodecyl sulphate, performed on choleragen after incubation with pronase, only showed the band corresponding to the B subunit, while the A subunit was lost. When examined in immunodiffusion, the digested choleragen was still able to precipitate with specific antibodies. On the other hand, toxicity was considerably reduced.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P. Tarli, A. Ricci, M. Porro and P. Neri, IRCS Med. Sci.3, 205 (1975).

    CAS  Google Scholar 

  2. G. Antoni, I. Marsili, P. Tarli and P. Neri, Experientia32, 971 (1976).

    Article  CAS  PubMed  Google Scholar 

  3. M. Saletti, A. Ricci and P. Neri, in: Proc. Symp. comb. Vaccines, Zagreb 1972, p. 75.

  4. V.K. Laemnli, Nature227, 680 (1970).

    Article  Google Scholar 

  5. O. Outcherlony, in: Handbook of Immunodiffusion and immunoelectrophoresis, Ann Arbor Sci. Publ., Ann Arbor. Michigan, USA.

  6. J.P. Craig, J. Bact.92, 793 (1966).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. O. Lowry, N.J. Rosebrough, A.L. Farr and R.J. Randall, J. biol. Chem.193, 265 (1951).

    Article  CAS  PubMed  Google Scholar 

  8. Y. Narahashi, Meth. Enzym.19, 651 (1970).

    Article  Google Scholar 

  9. S. Van Heyningen, J. inf. Dis.133, 55 (1976).

    Article  Google Scholar 

  10. P. Cuatrecasas, I. Parikh and M.D. Hollenberg, Biochemistry12, 4253 (1973).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. ISVT Sclavo, Research Centre, Via Fiorentina 1, I-53100, Siena, Italy

    P. Neri, G. Antoni & P. Tarli

Authors
  1. P. Neri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Antoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Tarli
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Neri, P., Antoni, G. & Tarli, P. Difference in resistance of subunits A and B ofVibrio cholerae toxin (choleragen) to treatment with pronase. Experientia 34, 1293–1294 (1978). https://doi.org/10.1007/BF01981426

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01981426

Keywords

Search

Navigation