Abstract
Heterozygosity in three cultivars of the blackcurrant (Ribes nigrum L.) was estimated. A selfed population of each cultivar was screened for Random Amplified Polymorphic DNA markers (RAPDs) and heterozygous loci were identified by band segregation in contrast with the non-segregation of homozygous loci. On average, 21% of the loci scored in each cultivar were heterozygous. The implications for mapping studies in Ribes nigrum are discussed.
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References
Anonymous, 1994. Production Yearbook. Food and Agriculture Organisation, United Nations, Rome, Vol. 48. Table 75, p. 168.
Brennan R.M., 1990. Currants and gooseberries (Ribes), pp. 457–488 in Genetic Resources of Temperate Fruit and Nut Crops, edited by J.N. Moore and J.R. Ballingten. ISHS Publications, Netherlands.
Brennan R.M., P.C. Lanham & R.J. McNicol, 1993. Ribes breeding and research in the UK. Acta Horticulturas 352: 267–275.
Byrne M., J.C. Murrell, B. Allen & G.F. Meran, 1995. An integrated genetic liakage map for eucalypts using RFLP, RAPD and isozyme markers. Theoretical and Applied Genetics 19: 869–875.
Hamrick J.L. & M.J.W. Godt, 1990. Allozyme diversity in plant species, pp. 43–63 in Plant Population Genetics, Breeding and Genetic Resources, edited by A.H.D. Brown, M.T. Clegg, A.L. Kahler and B.S. Weig. Sinaeur Association Massachusetts.
Keep, E., 1975. Currants and gooseberries, pp. 197–268 in Advances in Fruit Breeding, edited by J. Janick & J.N. Moore. Purdue University Press.
Kronenberg H.G. & K. Hofman, 1965. Research on some characters in blaekcurrant progenies. Euphytica 14: 23–35.
Lanaud C., A.M. Risterucci, A.K.J. N'Ooren, D. Clement, M.H. Flament, V. Laurent & M. Palque, 1995. A genetic linkage map of Theobroma cacao L. Theoretical and Applied Genetics 91: 937–993.
Lanham P.G., R.M. Brennan, C. Hackett & R.J. McNicol, 1995. RAPD fingerprinting of blackcurrant (Ribes nigrum L.) cultivars. Theoretical & Applied Genetics 90: 166–172.
Milligan B.G., 1992. Plant DNA Isolation, pp. 59–88 in Molecular Genetics of Populations: A Practical Approach, edited by A.R. Hoelzed. IRL Press, Oxford, UK.
Ritter E., C. Gebhardt & F. Salamini, 1990. Estimation of recombination frequencies and construction of RFLP linkage maps in plants from crosses between heterozygous parents. Genetics 125: 645–654.
Trajkovski, V., 1992. Currant and gooseberry breeding. Verksamhetsberättelse 1990–1991 Report. Balsgärd, pp. 41–45.
Viruel M.A., R. Messeguer, M.C.de Vicente, J. Garcia-Mas, P. Puigdomenech, F. Vargas & P. Arus, 1995. A linkage map with RFLP and isozyme markers for almond. Theoretical and Applied Genetics 91: 964–971.
Wilson D., 1970. Blackcurrant breeding a progeny test of four cultivars and a study of inbreeding effects. Journal of Horticultural Science 45: 239–247.
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Lanham, P.G. Estimation of heterozygosity in Ribes nigrum L. using RAPD markers. Genetica 98, 193–197 (1996). https://doi.org/10.1007/BF00121366
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DOI: https://doi.org/10.1007/BF00121366