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Organoid Culture: Applications in Development and Cancer

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Ex Vivo Engineering of the Tumor Microenvironment

Part of the book series: Cancer Drug Discovery and Development ((CDD&D))

Abstract

The culture of primary human tissues ex vivo has historically been laden with challenges. Following the successful establishment of multiple different carcinoma cell lines, most notably HeLa cells, which helped support viral research in the 1940s and 1950s, the difficulty of propagating normal diploid cells was recognized by multiple investigators. Despite using similar 2-dimensional (2D) culture techniques that have stood the test of time—adherent culture on petri dishes using Eagle’s Medium with balanced salt solutions, 10 % calf serum, and penicillin/streptomycin - Hayflick and Moorhead perhaps best defined the limits of studying normal human tissue in a dish in 1961. Upon mincing fetal tissue they could derive multiple human diploid fibroblast cultures, but invariably the number of subcultivations was limited below 60 passages, for which they coined the term cellular “senescence,” also commonly known as the “Hayflick Limit.”

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  1. Department of Medical Oncology, Dana Farber Cancer Institute, 450 Brookline Avenue, Boston, MA, 02215, USA

    Israel Cañadas & David A. Barbie

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    Amir R. Aref

  2. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA

    David Barbie

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Cañadas, I., Barbie, D.A. (2017). Organoid Culture: Applications in Development and Cancer. In: Aref, A., Barbie, D. (eds) Ex Vivo Engineering of the Tumor Microenvironment. Cancer Drug Discovery and Development. Humana Press, Cham. https://doi.org/10.1007/978-3-319-45397-2_3

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