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Preparation and Evaluation of Molecularly Imprinted Polymers for Promazine and Chlorpromazine by Multi-step Swelling and Polymerization: the Application for the Determination of Promazine in Rat Serum by Column-switching LC

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Abstract

Molecularly imprinted polymers (MIPs) for promazine (PZ) and chlorpromazine (CPZ), MIPPZ and MIPCPZ, were prepared by multi-step swelling and polymerization using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a crosslinker. The retention and molecular-recognition properties of MIPPZ and MIPCPZ were evaluated using a mixture of potassium phosphate buffer and acetonitrile, or a mixture of ammonium formate and acetonitrile as the mobile phase in LC. PZ and CPZ gave the maximal retentions on MIPPZ and MIPCPZ at an apparent pH 8.2 using a mixture of potassium phosphate buffer and acetonitrile as the mobile phase. The retentions of PZ and CPZ decreased with an increase of acetonitrile contents from 70 to 90 vol% using a mixture of ammonium formate and acetonitrile as the mobile phase. The template molecules (PZ and CPZ, respectively) were recognized the most on the respective MIPs, and the imprinting factor of PZ was higher on MIPCPZ than on MIPPZ. These results indicate that in addition to shape recognition, ionic and hydrophobic interactions seem to work for the retention and molecular-recognition of PZ and CPZ on the MIPs. MIPCPZ was successfully utilized for the selective extraction of PZ in rat-serum samples in columnswitching LC with fluorescence detection.

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Acknowledgments

This work was supported in part by JSPS KAKENHI Grant No. JP16K08212 to J. H.

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Authors and Affiliations

  1. Department of Pharmacy, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien Kyuban-cho, Nishinomiya, 663-8179, Japan

    Kanae Nishimura & Jun Haginaka

  2. Institute for Biosciences, Mukogawa Women's University, 11-68 Koshien Kyuban-cho, Nishinomiya, 663-8179, Japan

    Jun Haginaka

Authors
  1. Kanae Nishimura
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  2. Jun Haginaka
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Correspondence to Jun Haginaka.

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Nishimura, K., Haginaka, J. Preparation and Evaluation of Molecularly Imprinted Polymers for Promazine and Chlorpromazine by Multi-step Swelling and Polymerization: the Application for the Determination of Promazine in Rat Serum by Column-switching LC. ANAL. SCI. 35, 659–664 (2019). https://doi.org/10.2116/analsci.19P011

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  • DOI: https://doi.org/10.2116/analsci.19P011

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