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A new restricted access molecularly imprinted polymer capped with albumin for direct extraction of drugs from biological matrices: the case of chlorpromazine in human plasma

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Abstract

A new restricted access molecularly imprinted polymer coated with bovine serum albumin (RAMIP-BSA) was developed, characterized, and used for direct analysis of chlorpromazine in human plasma samples. The RAMIP-BSA was synthesized using chlorpromazine, methacrylic acid, and ethylene glycol dimethacrylate as template, functional monomer, and cross-linker, respectively. Glycerol dimethacrylate and hydroxy methyl methacrylate were used to promote a hydrophilic surface (high density of hydroxyl groups). Afterward, the polymer was coated with BSA using glutaraldehyde as cross-linker, resulting in a protein chemical shield around it. The material was able to eliminate ca. 99 % of protein when a 44-mg mL−1 BSA aqueous solution was passed through it. The RAMIP-BSA was packed in a column and used for direct analysis of chlorpromazine in human plasma samples in an online column switching high-performance liquid chromatography system. The analytical calibration curve was prepared in a pool of human plasma samples with chlorpromazine concentrations ranging from 30 to 350 μg L−1. The correlation coefficient obtained was 0.995 and the limit of quantification was 30 μg L−1. Intra-day and inter-day precision and accuracy presented variation coefficients and relative errors lower than 15 % and within −15 and 15 %, respectively. The sample throughput was 3 h−1 (sample preparation and chromatographic analysis steps) and the same RAMIP-BSA column was efficiently used for about 90 cycles.

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Acknowledgments

We thank the “Fundação de Amparo à Pesquisa do Estado de Minas Gerais” (Belo Horizonte, Brazil; projects CDS-APQ-01612-10 and CDS-APQ-01323-09), “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (Brasília, Brazil), and “Fundação de Amparo à Pesquisa do Estado de São Paulo” (São Paulo, Brazil; process 2007/50970-5).

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Authors and Affiliations

  1. Laboratory of Toxicant and Drug Analysis, Faculty of Pharmaceutical Sciences, Federal University of Alfenas—Unifal-MG, Gabriel Monteiro da Silva St. 700, 37130-000, Alfenas, MG, Brazil

    Gabriel de Oliveira Isac Moraes, Larissa Meirelles Rodrigues da Silva & Eduardo Costa Figueiredo

  2. Laboratory of Chromatography, Institute of Chemistry of São Carlos, University of São Paulo, P.O. Box 780, 13566-590, São Carlos, SP, Brazil

    Álvaro José dos Santos-Neto

  3. Instituto de Ciências Exatas, Federal University of Alfenas—Unifal-MG, Gabriel Monteiro da Silva St. 700, 37130-000, Alfenas, MG, Brazil

    Fábio Herbst Florenzano

Authors
  1. Gabriel de Oliveira Isac Moraes
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  2. Larissa Meirelles Rodrigues da Silva
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  3. Álvaro José dos Santos-Neto
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  4. Fábio Herbst Florenzano
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  5. Eduardo Costa Figueiredo
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Correspondence to Eduardo Costa Figueiredo.

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de Oliveira Isac Moraes, G., da Silva, L.M.R., dos Santos-Neto, Á.J. et al. A new restricted access molecularly imprinted polymer capped with albumin for direct extraction of drugs from biological matrices: the case of chlorpromazine in human plasma. Anal Bioanal Chem 405, 7687–7696 (2013). https://doi.org/10.1007/s00216-013-7275-5

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  • DOI: https://doi.org/10.1007/s00216-013-7275-5

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