Summary
A novel approach to solid phase extraction, based on the use of a highly selective molecularly imprinted polymer, is presented. The versatility of this type of sorbent for solid phase extraction was demonstrated in a model batch-wise pre-concentration of sameridine prior to gas chromatography. Problems associated with leakage of remaining imprint molecules during the desorption phase could be eliminated by the use of a close structural analogue of sameridine as the imprint species. It was found that a major benefit of the imprinted polymer was its specificity, which lead to distinctly cleaner chromatographic traces and ability to improve sensitivity by extracting sameridine from larger sample volumes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
G. Wulff, Angew. Chem. Int. Ed. Engl.34, 1812 (1995).
J. Steinke, D. C. Sherrington, I. R. Dunkin, Adv. Polym. Sci.123, 81 (1995).
K. Mosbach, O. Ramström, Bio/technology14, 163 (1996).
K. Mosbach, Trends Biochem. Sci.19, 9 (1994).
K. J. Shea, Trends Polym. Sci.2, 166 (1994).
S. Vidyasankar, F. H. Arnold, Curr. Opin. Biotechnol.6, 218. (1995).
R. J. Ansell, D. Kriz, K. Mosbach, Curr. Opin. Biotechnol.7, 89 (1996).
G. Vlatakis, L. I. Andersson, R. Müller, K. Mosbach, Nature361, 645 (1993).
L. I. Andersson, Anal. Chem.68, 111 (1996).
L. I. Andersson, K. Mosbach, J. Chromatrogr.516, 313 (1990).
G. Wulff, M. Minarik, J. Liq. Chromatogr.13, 2987 (1990).
L. Fischer, R. Müller, B. Ekberg, K. Mosbach, J. Am. Chem. Soc.113, 9358 (1991).
B. Sellergren, K. J. Shea, J. Chromatogr.635, 31 (1993).
O. Ramström, I. A. Nicholls, K. Mosbach, Tetrahedron: Assymetry5, 649 (1994).
M. Kempe, K. Mosbach, J. Chromatogr.664, 276 (1994).
J. Matsui, I. A. Nicholls, T. Takeuchi, Tetrahedron: Assymetry7, 1357 (1996).
M. Kempe, Anal. Chem.68, 1948 (1996).
A. G. Mayes, K. Mosbach, Anal. Chem.68, 3769 (1996).
L. Schweitz, L. I. Andersson, S. Nilsson, Anal. Chem.69, 1179 (1997).
R. Sandberg, A.-L., Ask, Synthesis and structure-activity relationship of some 1-alkyl-4-phenyl-4-piperidine-carboxamides regarding spinal anesthetic/analgesic effects. J. Med. Chem. Manuscript, submitted for publication.
P. Neidenström, C. Norsten-Höög, T. Arvidsson, J. Chromatogr. submitted for publication.
M. T. Muldoon, L. H. Stanker, Anal. Chem.69, 803 (1997).
P. Martin, I. D. Wilson, D. E. Morgan, G. R. Jones, K. Jones, Anal. Commun.34, 45 (1997).
T. Arvidsson, C. Norsten-Höög, E. Eklund, Determination of free concentration of sameridine in blood plasma by ultrafiltration and coupled-column liquid chromatography. J. Chromatogr. submitted for publication.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Andersson, L.I., Paprica, A. & Arvidsson, T. A highly selective solid phase extraction sorbent for pre-concentration of sameridine made by molecular imprinting. Chromatographia 46, 57–62 (1997). https://doi.org/10.1007/BF02490930
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02490930