Abstract
The inhibitory effects of Lactobacillus plantarum-fermented and non-fermented Inula britannica extracts on the tyrosinase activity were comparatively investigated to examine whether and how they improve the whitening activity, and the contents of total flavonoids and polyphenolics as bioactive compounds were determined. The skin whitening activity using in vitro or ex vivo tyrosinase and L-3,4-dihydroxyphenylalanine (L-DOPA) staining was examined. The total flavonoid content (TFC) was increased by 13.4% after 72 h-fermentation. The viabilities of the B16F10 cells treated with the fermented and non-fermented control extracts were 100.26% and 92.15% at 500 μg/ml, respectively. In addition, the inhibition of tyrosinase activity was increased by the fermented samples from 29.33% to 41.74% following fermentation for up to 72 h. The tyrosinase activity of the untreated control group was increased to 145.69% in B16F10 cells. The results showed that I. britannica fermented by L. plantarum dose-dependently inhibited tyrosinase activity, which was stimulated by α-melanocyte stimulating hormone. These results suggest that lactic fermented I. britannica extracts can be used as effective skin-whitening materials.
中文概要
目的
研究植物乳杆菌发酵对旋覆花花瓣提取物对酪氨 酸酶的抑制效果,为开发旋覆花提取物作为潜在 的皮肤增白成分提供依据。
创新点
发现旋覆花植物乳杆菌发酵的提取物可以作为有 效的美白材料。
方法
比较研究了植物乳杆菌发酵的旋覆花花瓣提取物 对酪氨酸酶活性的抑制作用,并测定了提取物中 总黄酮和多酚类化合物的含量。通过体外酪氨酸 酶和左旋多巴(L-DOPA)染色来测定其对皮肤 的美白活性。
结论
旋覆花花瓣经过72 小时的发酵后,其提取物中 总黄酮含量提高13.4%。采用500 μg/ml 发酵和未 发酵的提取物处理B16F10 细胞,细胞活性分别 为100.26%和92.15%。此外,发酵72 小时后, 该提取物对酪氨酸酶活性的抑制率由29.33%上 升到41.74%。未处理的对照组B16F10 细胞酪氨 酸酶活性增加到145.69%。结果表明,旋覆花发 酵提取物对酪氨酸酶活性呈剂量依赖性抑制是 促黑激素(α-MSH)的刺激引起。旋覆花植物乳 杆菌发酵的提取物可以作为有效的美白材料。
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Project supported by the Agriculture Bio-industry Technology Development Program, Ministry of Agriculture, Food and Rural Affairs of Korea (No. 314020-2), the Ministry for Food, Agriculture, Forestry and Fisheries of Korea (No. 614102-2), and the National Research Foundation of Korea (No. 2009-0093824)
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Park, Eh., Bae, Wy., Kim, Jy. et al. Antimelanogenic effects of Inula britannica flower petal extract fermented by Lactobacillus plantarum KCCM 11613P. J. Zhejiang Univ. Sci. B 18, 816–824 (2017). https://doi.org/10.1631/jzus.B1600234
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DOI: https://doi.org/10.1631/jzus.B1600234