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Green synthesis of R-phycoerythrin protected gold nanoclusters for sensitive detection of mercury(II) ions and their antibacterial properties

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Abstract

Gold nanoclusters (AuNCs) occupy a distinct place in the class of transition metal nanoclusters owing to their ultrasmall size, photostability and non-toxicity in biomedical applications. In this work, R-phycoerythrin (RPE) was extracted from Spyridia filamentosa and used as a stabilizing and reducing agent for the green synthesis of AuNCs. As-synthesized RPE-stabilized AuNCs exhibited pH-tuned stability and surface morphology. The average particle size of AuNCs is estimated to be 4.8 nm by HR-TEM analysis. The antibacterial activity of RPE-AuNCs against medically significant bacteria was determined using agar well diffusion method. The minimum inhibitory concentration (MIC) of RPE-AuNCs against Staphylococcus aureus and Escherichia coli were 2 mg/mL and 50 μg/mL, respectively. The as-synthesized RPE-AuNCs are applicable as a fluorescent probe for the detection of Hg2+ ion with a detection limit of 5.2 nM. These results demonstrate the green synthesized R-phycoerythrin-protected AuNCs for their potential applications in biomedical and sensing fields.

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The datasets generated during and/or analyzed during the current studies are available from the corresponding author on reasonable request.

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Acknowledgments

This work was rendered by Department of Science and Technology (DST)-Innovation in Science Pursuit for Inspired Research (INSPIRE) fellowship programme (IF170915). The authors wish to acknowledge the funding from Research Innovative and Quality Improvement (RI & QI), RUSA 2.0, University of Madras. We also acknowledge the GNR Instrumentation Centre, University of Madras for PL, IR, Raman, DLS, and Zeta potential analysis.

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Authors and Affiliations

  1. Department of Physical Chemistry, School of Chemical Science, University of Madras, Guindy Campus, Chennai, Tamilnadu, 600 025, India

    Vinitha Packirisamy & Prabhu Pandurangan

  2. Department of Centre for Advanced Study in Botany, University of Madras, Guindy Campus, Chennai, Tamilnadu, 600 025, India

    Brabakaran Ayyadurai, Jayappriyan Kothilmozhian Ranishree & Thangaraju Nallamuthu

  3. Department of Microbiology, University of Madras, Taramani Campus, Chennai, Tamilnadu, 600 113, India

    Damodharan Perumal & Prabu Dhandapani

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  1. Vinitha Packirisamy
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Packirisamy, V., Ayyadurai, B., Perumal, D. et al. Green synthesis of R-phycoerythrin protected gold nanoclusters for sensitive detection of mercury(II) ions and their antibacterial properties. Journal of Materials Research 38, 3009–3021 (2023). https://doi.org/10.1557/s43578-023-01021-w

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