Skip to main content
SpringerLink
Log in

Breast cancer detection using charge sensors coupled to DNA monolayer

  • Articles
  • Published:
MRS Online Proceedings Library Aims and scope

Abstract

We report the development of a label-free biosensors based on DNA hybridization, using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This study uses DNA sequences based on microRNA related with breast cancer. The biosensor was fabricated by immobilizing a self-assembled monolayer of single-stranded 23-mer oligonucleotide (ssDNA) via a thiol linker on gold work electrodes. Residual binding places were filled with 6 -mercaptohexanol (MCH). The electrode was electrochemicaly characterized in the presence of a redox system ferri/ferrocyanide. Different concentrations of complementary DNA sequence for hybridization were incubated; an increase of charge transfer resistance (Rct) was observed, used as sensor parameter and correlated with concentrations of complementary DNA sequence. A debate was presented on the effect of the MgCl2 influence on ssDNA immobilization solution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. T. G. Drummond, M. G. Hill and J. K. Barton. Nature Biotechnology 21, 1192 – 1199 (2003).

    Article  CAS  Google Scholar 

  2. J. Reichert, A. Csaki, J.M. Kohler,W. Fritzsche. Anal. Chem. 72, 6025 (2000).

    Article  CAS  Google Scholar 

  3. F. Caruso, E. Rodda, D.N. Furlong, K. Niikura, Y. Okahata. Anal. Chem. 69, 2043 (1997).

    Article  CAS  Google Scholar 

  4. B.P. Nelson, T.E. Grimsrud, M.R. Liles, R.M. Goodman, R.M. Corn. Anal. Chem. 73, 1 (2001).

    Article  CAS  Google Scholar 

  5. Z.L. Zhang, D.W. Pang, H. Yuan, R.X. Cai, H. Abruna. Anal. Bioanal. Chem. 381, 833 (2005).

    Article  CAS  Google Scholar 

  6. J. Wang, Electrochemical nucleic acid biosensors, in: E. Palecek, F. Scheller, J. Wang, Elsevier, 175 (2005).

  7. J.J. Gooding, Electroanalysis 14, 1149 (2002).

    Article  CAS  Google Scholar 

  8. M. Mir1, S. M. Rodríguez, O. C. Fernández, A. H. Corbera, J. Samitier and P. Wynblatt, Electrophoresis 32, 811–821 (2011).

    Article  CAS  Google Scholar 

  9. A. Esquela-Kerscher and F.J. Slack. Nat. Rev. Cancer 6, 259–269 (2006).

    Article  CAS  Google Scholar 

  10. Y. Wen et al. Methods 64, 276–282(2013).

    Article  CAS  Google Scholar 

  11. U. Nagalakshmi, K. Waern, and M. Snyder. Current Protocols in Molecular Biology 4.11.1–4.11.13 (2010).

  12. A. Kukol, P. Li, P. Estrela, P. Ko-Ferrigno and P. Migliorato. Anal. Biochem. 374. 143–153 (2008).

    Article  CAS  Google Scholar 

  13. J. Kafka, O. Panke, B. Abendroth and F. Lisdat. Electrochimica Acta 53, 7467–7474 (2008).

    Article  CAS  Google Scholar 

  14. A. B. Steel, T, M. Herne, and M. J. Tarlov. Anal. Chem. 70, 4670–4677 (1998).

    Article  CAS  Google Scholar 

  15. L. M. Fiscger. Microelectronic engineering 86, 1282 – 1285 (2009).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Sao Paulo, 14040-901, Brazil

    Marina R. Batistuti & Marcelo Mulato

  2. Instituto de Química, Universidade Estadual Paulista, CP 355, Araraquara, São Paulo, 14800-900, Brazil

    Paulo R. Bueno

Authors
  1. Marina R. Batistuti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marcelo Mulato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paulo R. Bueno
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Batistuti, M.R., Mulato, M. & Bueno, P.R. Breast cancer detection using charge sensors coupled to DNA monolayer. MRS Online Proceedings Library 1793, 19–26 (2015). https://doi.org/10.1557/opl.2015.671

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1557/opl.2015.671

Search

Navigation