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Electrochemical DNA biosensors for label-free breast cancer gene marker detection

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Abstract

We present an electrochemical DNA detection strategy based on self-assembled ferrocene-cored poly(amidoamine) dendrimers for the detection of a gene relevant to breast cancer. The chemisorption of three ferrocene-cored poly(amidoamine) generations and hybridization of single-stranded DNA on a Au electrode were studied by cyclic voltammetry and differential pulse voltammetry. The biosensor demonstrated high sensitivity of 0.13 μA/(ng/ml) in the detection of the target DNA with a linear range of 1.3–20 nM and a detection limit of 0.38 nM. The DNA biosensor also has high selectivity for the target DNA, showing a clear signal difference from a noncomplementary sequence and a single-base-mismatch sequence, which was used as a model of BRAC1 gene mutation. The results shown are highly motivating for exploring DNA biosensing technology in the diagnosis of breast cancer caused by mutation of the BRAC1 gene.

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Acknowledgements

Some of this work was performed at the former Fatih University as a part of the work presented in FK’s master thesis.

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Authors and Affiliations

  1. SANKARA Brain & Biotechnology Research Center, Nanoyasam Nanobiotechnology Company, Technocity, Avcılar, 34320, Istanbul, Turkey

    Mehmet Senel

  2. Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, 3052, Australia

    Muamer Dervisevic

  3. Dabska 18H 52-571, Kraków, Poland

    Firdevs Kokkokoğlu

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  1. Mehmet Senel
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  2. Muamer Dervisevic
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Correspondence to Mehmet Senel.

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Senel, M., Dervisevic, M. & Kokkokoğlu, F. Electrochemical DNA biosensors for label-free breast cancer gene marker detection. Anal Bioanal Chem 411, 2925–2935 (2019). https://doi.org/10.1007/s00216-019-01739-9

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