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Application of graphene oxide nanosheets as probe oligonucleotide immobilization platform for DNA sensing

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Abstract

In this work, a simple and novel electrochemical biosensor based on a glassy carbon electrode (GCE) modified with graphene oxide nanosheets (GO) was developed for detection of DNA sequences. The morphology of prepared nanoplatform was investigated by scanning electron microscopy, infrared (FTIR) and UV/Vis absorption spectra. The fabrication processes of electrochemical biosensor were characterized with cyclic voltammetry and electrochemical impedance spectroscopy (EIS) in an aqueous solution. The optimization of experimental conditions such as immobilization of the probe BRCA1 and its hybridization with the complementary DNA was performed. Due to unique properties of graphene oxide nanosheets such as large surface area and high conductivity, a wide liner range of 1.0 × 10−17–1.0 × 10−9 M and detection limit of 3.3 × 10−18 M were obtained for detection of BRCA1 5382 mutation by EIS technique. Under the optimum conditions, the proposed biosensor (ssDNA/GO/GCE) revealed suitable selectivity for discriminating the complementary sequences from non-complementary sequences, so it can be applicable for detection of breast cancer.

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Acknowledgments

We gratefully acknowledge the support of this work by Yazd University research council.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Yazd University, Yazd, Islamic Republic of Iran

    Ali Benvidi, Marzieh Dehghan Tezerjani, Afsaneh Dehghani Firouzabadi & Mohammad Mazloum-Ardakani

  2. Department of Biology, Faculty of Science, Yazd University, Yazd, Islamic Republic of Iran

    Seyed Mohammad Moshtaghioun

Authors
  1. Ali Benvidi
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  2. Marzieh Dehghan Tezerjani
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  3. Afsaneh Dehghani Firouzabadi
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  4. Mohammad Mazloum-Ardakani
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  5. Seyed Mohammad Moshtaghioun
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Correspondence to Ali Benvidi.

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Benvidi, A., Tezerjani, M.D., Firouzabadi, A.D. et al. Application of graphene oxide nanosheets as probe oligonucleotide immobilization platform for DNA sensing. J IRAN CHEM SOC 13, 2135–2142 (2016). https://doi.org/10.1007/s13738-016-0931-x

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  • DOI: https://doi.org/10.1007/s13738-016-0931-x

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