Abstract
Nearly twenty thousand wheat lines were phenotyped for the presence of leaf tip necrosis (LTN), a phenotypic trait linked to adult plant leaf rust resistance (APR) genes, viz. Lr34, Lr46 and Lr67 having pleiotropic association with multiple disease resistance genes. Thirty-six lines showed varied expression of LTN and moderate level of leaf rust severity at adult plant stage with slow rusting (disease progress at a retarded rate). Seedling resistance test (SRT) revealed susceptible and mixed infection types, a characteristic of adult plant resistance (APR) genes. Further molecular confirmation for the presence of these genes using available microsatellite markers revealed that of the 36 lines, five lines carried Lr46 + alone and five other lines carried Lr67 + alone. Seven lines carried the combination of Lr34 + and Lr67 + while six lines confirmed to carry the combination of Lr46 + and Lr67 +. Remarkably three lines carried all the three APR genes, viz. Lr34 +, Lr46 + and Lr67 +. All these stocks can be a source of APR multiple disease resistance genes. Ten lines were not confirmed to carry any of the genes but still had LTN and SRT results showing an infection type typical of APR genes and these can be the source of identifying newer APR genes. The resistance based on minor APR genes when combined with a few additional minor genes in the background of high yielding cultivars is expected to have high level of race non-specific resistance and to be durable.
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Agarwal, S., Saini, R.G. 2009. Undescribed wheat gene for partial leaf rust and stripe rust resistance from Thatcher derivatives RL6058 and 90RN2491 carrying Lr34. J. Appl. Genet. 50:199–204.
Bolton, M.D., Kolmer, J.A., Xu, W.W., Garvin, D.F. 2008. Lr34-mediated leaf rust resistance in wheat: Transcript profiling reveals a high energetic demand supported by transient recruitment of multiple metabolic pathways. Mol. Plant Microbe Interact. 21:1515–1527.
Borghi, B. 2001. Italian wheat pool. In: Bonjean, A.P., Angus, W.J. (eds), The World Wheat Book: A History of Wheat Breeding. Intercept, London, UK, pp. 289–309.
Broers, L.H.M. 1989. Influence of development stage and host genotype on three components of partial resistance to leaf rust in spring wheat. Euphytica 44:187–195.
Caldwell, R.M. 1968. Breeding for general and/or specific plant disease resistance. In: Finlay, K.W., Shephard, K.W. (eds), Proc. 3rd Int. Wheat Genetics Symp. Australian Academy of Sciences, Canberra, Australia, pp. 263–272.
Chen, X.M., Jones, S., Line, R.F. 1995. Chromosomal location of genes for stripe rust resistance in spring wheat cultivars Compair, Fielder, Lee, and Lemhi and interactions of aneuploid wheat with races of Puccinia striiformis. Phytopathol. 85:375–381.
Dyck, P.L. 1977. Genetics of leaf rust reaction in three introductions of common wheat. Canadian J. Gen. Cytol. 19:711–716.
Dyck, P.L. 1987. The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29:467–469.
Dyck, P.L., Samborski, D.J. 1982. The inheritance of resistance to Puccinia recondita in a group of common wheat cultivars. Can. J. Genet. Cytol. 24:273–283.
Hiebert, C.W., Thomas, J.B., McCallum, B.D., Humphreys, D.G., DePauw, R.M., Hayden, M.J., Mago, R., Schnippenkoetter, W., Spielmeyer, W. 2010. An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67). Theor. Appl. Genet. 121:1083–1091.
Jacobs, T., Buurlage, M.B. 1990. Growth of wheat leaf rust colonies in susceptible and partially resistant spring wheats. Euphytica 45:71–80.
Kolmer, J.A. 2005. Tracking wheat rust on a continental scale. Curr. Opin. Plant Biol. 8:441–449.
Kolmer, J.A., Singh, R.P., Garvin, D.F., Viccars, L., William, H.M., Huerta-Espino, J., Ogbonnaya, F.C., Raman, H., Orford, S., Bariana, H.S., Lagudah, E.S. 2008. Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm. Crop Sci. 48:1841–1852.
Krattinger, S.G., Lagudah, E.S., Spielmeyer, W., Singh, R.P., Huerta-Espino, J., McFadden, H., Bossolini, E., Selter, L.L., Keller, B. 2009. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363.
Lagudah, E.S. 2010. Molecular genetics of race non-specific rust resistance in wheat. In: BGRI Technical Workshop, 30–31 May, St Petersburg, Russia, pp. 184–196.
Lagudah, E.S. 2011. Molecular genetics of race non-specific rust resistance in wheat. Euphytica 179:81–91.
Lagudah, E.S., McFadden, H., Singh, R.P., Huerta-Espino, J., Bariana, H.S., Spielmeyer, W. 2006. Molecular genetic characterisation of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor. Appl. Genet. 114:21–30.
Lagudah, E.S., Krattinger, S.G., Herrera-Foessel, S., Singh, R.P., Huerta-Espino, J., Spielmeyer, W., Brown-Guedira, G., Selter, L.L., Keller, B. 2009. Gene-specific markers for the wheat gene Lr34/Yr18/ Pm38 which confers resistance to multiple fungal pathogens. Theor. Appl. Genet. 119:889–898.
Long, D.L., Kolmer, J.A. 1989. A north American system of nomenclature for Puccinia triticina. Phytopathol. 79:525–529.
Ma, H., Singh, R.P. 1996. Contribution of adult plant resistance gene Yr18 in protecting wheat from yellow rust. Plant Dis. 80:66–69.
Martínez, F., Niks, R.E., Singh, R.P., Rubiales, D. 2001. Characterization of Lr46, a gene conferring partial resistance to wheat leaf rust. Hereditas 135:111–114.
McCallum, B.D., Seto-Goh, P. 2005. Physiologic specialization of wheat leaf rust (Puccinia triticina) in Canada in 2002. Can. J. Plant Pathol. 27:90–99.
McGregor, A.J., Manners, J.G. 1985. The effect of temperature and light intensity on growth and sporulation of Puccinia striiformis on wheat. Plant Pathol. 34:263–271.
McIntosh, R.A. 1992. Close genetic linkage of genes conferring adultplant resistance to leaf rust and stripe rust in wheat. Plant Pathol. 41:523–527.
Messmer, M.M., Seyfarth, R., Keller, M., Schachermayr, G., Winzeler, M., Zanetti, S., Feuillet, C., Keller, B. 2000. Genetic analysis of durable leaf rust resistance in winter wheat. Theor. Appl. Genet. 100:419–431.
Nayar, S.K., Prashar, M., Bhardwaj, S.C. 1997. Manual of current techniques in wheat rusts. Directorate of Wheat Research, Regional Station, Flowerdale, Shimla, India. Research Bulletin No. 2, p. 18.
Parlevliet, J.E. 1975. Partial resistance of barley to leaf rust, Puccinia hordei. I. Effect of cultivar and development stage on latent period. Euphytica 24:21–27.
Peterson, R.F., Campbell, A.B., Hannah, A.E. 1948. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can. J. Res. 26:496–500.
Prabhu, K.V., Gupta, S.K., Charpe, A., Koul, S. 2008. SCAR marker tagged to the alien leaf rust resistance gene Lr19 uniquely marking the Agropyron elongatum-derived gene Lr24 in wheat. Plant Breed. 123:417–420.
Roelfs, A.P., Singh, R.P., Saari, E.E. 1992. Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico, pp. 1–81.
Rosewarne, G.M., Singh, R.P., Huerta-Espino, J., William, H.M., Bouchet, S., Cloutier, S., McFadden, H., Lagudah, E.S. 2006. Leaf tip necrosis, molecular markers and beta1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theor. Appl. Genet. 112:500–508.
Saari, E.E., Prescott, J.M. 1985. World distribution in relation to economic losses. In: Roelfs, A.P., Bushnell, W.R. (eds), The Cereal Rusts, Vol. 2. Diseases, distribution, epidemiology, and control. Academic Press, Orlando, FL, USA, pp. 259–298.
Singh, R.P. 1992. Association between gene Lr34 for leaf rust resistance and leaf tip necrosis in wheat. Crop Sci. 32:874–878.
Singh, R.P. 1993. Genetic association of gene Bdv1 for tolerance to barley yellow dwarf virus with genes, Lr34 and Yr18 for APR to rusts in bread wheat. Plant Disease 77:1103–1106.
Singh, R.P., Gupta, A.K. 1992. Expression of wheat leaf rust resistance gene Lr34 in seedlings and adult plants. Plant Dis. 76:489–491.
Singh, R.P., Rajaram, S. 1992. Genetics of adult-plant resistance to leaf rust in ‘Frontana’ and three CIMMYT wheats. Genome 35:24–31.
Singh, R.P., Huerta-Espino, J., William, H.M. 2005. Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turk. J. Agric. 29:121–127.
Singh, R.P., Mujeeb-Kazi, A., Huerta-Espino, J. 1998. Lr46: A gene conferring slow-rusting resistance to leaf rust in wheat. Phytopathol. 88:890–894.
Stubbs, R.W., Prescott, J.M., Saari, E.E., Dubin, H.J. 1986. Cereal disease methodology manual. Mexico, DF, CIMMYT, 46 pp.
Suenaga, K., Singh, R.P., Huerta-Espino, J., William, H.M. 2003. Microsatellite markers for genes Lr34/ Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathol. 93:881–890.
Wilcoxon, R.D. 1981. Genetics of slow rusting in cereals. Phytopathol. 71:989–993.
William, M., Singh, R.P., Huerta-Espino, J., Ortiz Islas, S., Hoisington, D. 2003. Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathol. 93:153–159.
Zadoks, J.C., Chang, T.T., Konzak, C.F. 1974. A decimal code for the growth stages of cereals Weed Res. 14:415–421.
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Sivasamy, M., Aparna, M., Kumar, J. et al. Phenotypic and Molecular Confirmation of Durable Adult Plant Leaf Rust Resistance (APR) Genes Lr34 +, Lr46 + and Lr67 + Linked to Leaf Tip Necrosis (LTN) In Select Registered Indian Wheat (T. aestivum) Genetic Stocks. CEREAL RESEARCH COMMUNICATIONS 42, 262–273 (2014). https://doi.org/10.1556/CRC.2013.0054
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DOI: https://doi.org/10.1556/CRC.2013.0054