Abstract
In this work gliadin proteins were used to analyse the genetic variability in a sample of the durum wheat Spanish collection conserved at the CRF-INIA. In total 38 different alleles were identified at the loci Gli-A1, Gli-A3, Gli-B5, Gli-B1, Gli-A2 and Gli-B2. All the gliadin loci were polymorphic, possessed large genetic diversity and small and large differentiation within and between varieties, respectively. The Gli-A2 and Gli-B2 loci were the most polymorphic, the most fixed within varieties and the most useful to distinguish among varieties. Alternatively, Gli-B1 locus presented the least genetic variability out of the four main loci Gli-A1, Gli-B1, Gli-A2 and Gli-B2. The Gli-B1 alleles coding for the gliadin γ-45, associated with good quality, had an accumulated frequency of 69.7%, showing that the Spanish germplasm could be a good source for breeding quality. The Spanish landraces studied showed new gliadin alleles not catalogued so far. These new alleles might be associated with specific Spanish environment factors. The large number of new alleles identified also indicates that durum wheat Spanish germplasm is rather unique.
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References
M.J. Asins and E.A. Carbonell, Distribution of genetic variability in a durum wheat world collection. Theor. Appl. Genet. 77 (1989) 287-294
J.M. Carrillo, J.F. Vázquez and J. Orellana, Relationship between gluten strength and glutenin proteins in durum wheat cultivars. Plant Breed. 104 (1990) 325-333
T. Damidaux, J.C. Autran, L. Grignac and P. Feillet, Mise en évidence de relations applicables en sélection entre lȁ9électrophorégramme des gliadins et les propriétés viscoélastiques du gluten de Triticum durum Desf. C.R. Acad. Sci. Ser. D 287 (1978) 701-704
I. Felix, J.P. Martinant, M. Bernard, S. Bernard and G. Branlard, Genetic characterization of storage proteins in a set of F1-derived haploid lines in bread wheat. Theor. Appl. Genet. 92 (1996) 340-346
A.M. Kudryavtsev, Genetics of gliadin of spring durum wheat (Triticum durum Desf.). Russian J. Genet. 30 (1994) 69-75
A.M Kudryavtsev, G. Boggini, S. Benedettelli and N.N. Illichevskii, Gliadin polymorphism and genetic diversity of modern Italian durum wheat. J. Genet. Breed. 50 (1996) 239-248
D. Lafiandra and D.D. Kasarda, One and two-dimensional (two-pH) polyacrylamide gel electrophoresis in a single gel: separation of wheat proteins. Cereal Chem. 62 (1985) 314-319
M.C. Martinez, Prolamins in durum wheat: inheritance and relation with pasta quality parameters. Madrid: Ingenieros Agrónomos (2001).
E.V. Metakovsky, Gliadin allele identification in common wheat. 2. Catalogue of gliadin alleles in common wheat. J. Genet. Breed. 45 (1991) 325-344
E.V. Metakovsky and G. Branlard, Genetic diversity of French common wheat germplasm studied using gliadin alleles. Theor. Appl. Genet. 96 (1998) 209-218
E.V. Metakovsky, M.G. Akhmedov and A.A. Sozinov, The genetic analysis of gliadin-encoding genes reveals clusters and remote genes. Theor. Appl. Genet. 73 (1986) 278-285
E.V. Metakovsky, D. Knezevic and B. Javornik, Gliadin allele composition of Yugoslav winter wheat cultivars. Euphytica 54 (1991) 285-295
E.V. Metakovsky, M. Gomez, J.F. Vazquez and J.M. Carrillo, Large genetic diversity of Spanish common wheat as judged from gliadin alleles. Plant Breed. 119 (2000) 37-42
E.V. Metakovsky, N.E. Pogna, A.M. Biancardi and R. Redaelli, Gliadin allele composition of common wheat cultivars grown in Italy. J. Genet. Breed. 48 (1994) 55-66
E.V. Metakovsky, C.W. Wrigley, F. Bekes and R.B. Gupta, Gluten polypeptides as useful genetic markers of dough quality in Australian wheats. Aust. J. Agric. Res. 41 (1990) 289-306
E.V. Metakovsky, P.K.W. Ng, V.M. Chernakov, N.E. Pogna and W. Bushuk, Gliadin alleles in Canadian western red strong wheat cultivars: use of two different procedures of acid polyacrylamide gel electrophoresis for gliadin separation. Genome 36 (1993) 743-749
E.V. Metakovsky, V.M. Chernakov, V.P. Upelniek, R. Redaelli, M. Dardevet, G. Branlard and N.E. Pogna, Recombination mapping of minor ω-gliadin –coding loci on chromosome 1A of common wheat: a revision. J. Genet. Breed. 50 (1996) 277-286
M. Nei, Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70 (1973) 3321-3323
M.T. Nieto-Taladriz, M. Ruiz, M.C. Martinez, J.F. Vazquez and J.M. Carrillo, Variation and classification of B low-molecular-weight glutenin subunit alleles in durum wheat. Theor. Appl. Genet. 95 (1997) 1155-1160
P.I. Payne, L.M. Holt, C.J. Lawrence and C.N. Law, The genetics of gliadin and glutenin the major storage proteins of the wheat endosperm. Qualit. Plant Foods Hum. Nutr. 31 (1982) 229-241
E.N. Pogna, E.V. Metakovsky, R. Redaelli, F. Raineri and T. Dachkevitch, Recombination mapping of Gli-5, a new gliadin-coding locus on chromosomes 1A an 1B in common wheat. Theor. Appl. Genet. 87 (1993) 113-121
J.S. Rogers, Measures of genetic similarity and genetic distance. Austin, TX: University of Texas (1972).
F.J. Rohlf, NTSYS-pc Numerical Taxonomy and Multivariate Analysis System. NewYork: Exeter software (1992).
M. Ruiz, Genetic analysis of the gluten proteins and their relation with quality in durum wheat (Triticum turgidum L.). Madrid: Ingenieros Agrónomos (1993).
M. Ruiz and J.M. Carrillo, Linkage relationships between prolamin genes on chromosomes 1A and 1B in durum wheat. Theor. Appl. Genet. 87 (1993) 353-360
M. Ruiz, E.V. Metakovsky, M. Rodriguez-Quijano, J.F. Vazquez and J.M. Carrillo, Assessment of storage protein variation in relation to some morphological characters in a sample of Spanish landraces of common wheat (Triticum aestivum L. ssp. vulgare). Genet. Resour. our. Crop Evol. 49 (2002a) 371-382
M. Ruiz, M. Rodriguez-Quijano, E.V. Metakovsky, F. Vazquez and J.M. Carrillo, Polymorphismvariation and genetic identity of Spanish common wheat germplasm based on gliadin alleles. Field Crops Res. 79 (2002b) 185-196
T.I. Sobko, Identification of a new locus which controls the synthesis of alcohol-soluble endosperm proteins in soft winter wheat. J. Agric. Sci. Kiev 7 (1984) 78-80
S. Wright, Evolution and the Genetics of Populations, vol. 4. Variability within and Among Natural Populations. Chicago: University of Chicago Press (1978).
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Aguiriano, E., Ruiz, M., Fité, R. et al. Analysis of Genetic Variability in a Sample of the Durum Wheat (Triticum durum Desf.) Spanish Collection Based on Gliadin Markers. Genet Resour Crop Evol 53, 1543–1552 (2006). https://doi.org/10.1007/s10722-005-7767-z
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DOI: https://doi.org/10.1007/s10722-005-7767-z