Skip to main content
SpringerLink
Log in

Determination of Mycoplasma hyopneumoniae-Specific IgG, IgG1, and IgG2a Titers in BALB/c Mice Induced by Mineral Oil-Based Oil-in-Water Emulsion Adjuvants Prepared Using a Self-Emulsifying Drug Delivery System

  • Research Article
  • Published:
AAPS PharmSciTech Aims and scope Submit manuscript

Abstract

We prepared mineral oil-based emulsion adjuvants by employing simple self-emulsifying drug delivery system (SEDDS). Mineral oil emulsions (3%, 5%, and 7%) were prepared using deionized water and C-971P NF and C-940 grade carbomer solutions with concentrations 0.01% (w/v) and 0.02% (w/v). In total, 15 emulsions were prepared and mixed with a solution containing inactivated Mycoplasma hyopneumoniae (J101 strain) antigen and porcine circovirus type 2 antigen to prepare vaccines. Droplet sizes in the submicron range and zeta potential values between − 40 and 0 mV were maintained by most emulsion adjuvants for a period of 6 months. Emulsion adjuvants were regarded safe, and their M. hyopneumoniae-specific IgG, IgG1, and IgG2a titers were either better or comparable to those of aluminum gel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Bastola R, Noh G, Keum T, Bashyal S, Seo JE, Choi J, et al. Vaccine adjuvants: smart components to boost the immune system. Arch Pharm Res. 2017;40(11):1238–48.

    Article  CAS  Google Scholar 

  2. Bobbala S, Hook S. Is there an optimal formulation and delivery strategy for subunit vaccines? Pharm Res. 2016;33(9):2078–97.

    Article  CAS  Google Scholar 

  3. Iyer V, Cayatte C, Marshall JD, Sun J, Schneider-Ohrum K, Maynard SK, et al. Feasibility of freeze-drying oil-in-water emulsion adjuvants and subunit proteins to enable single-vial vaccine drug products. J Pharm Sci. 2017;106(6):1490–8.

    Article  CAS  Google Scholar 

  4. Brito LA, Malyala P, O’Hagan DT. Vaccine adjuvant formulations: a pharmaceutical perspective. Semin Immunol. 2013;25:130–45.

    Article  CAS  Google Scholar 

  5. Thapa RK, Choi HG, Kim JO, Yong CS. Analysis and optimization of drug solubility to improve pharmacokinetics. J Pharm Investig. 2017;47(2):95–110.

    Article  CAS  Google Scholar 

  6. Wang Z, Pal R. Enlargement of nanoemulsion region in pseudo-ternary mixing diagrams for a drug delivery system. J Surfactant Deterg. 2014;17(1):49–58.

    Article  CAS  Google Scholar 

  7. Lee S, Lee J, Choi YW. Design and evaluation of prostaglandin E1 (PGE1) intraurethral liquid formulation employing self-microemulsifying drug delivery system (SMEDDS) for erectile dysfunction treatment. Biol Pharm Bull. 2008;31(4):668–72.

    Article  CAS  Google Scholar 

  8. Stipkovits L, Czifra G, Sundquist B. Indirect ELISA for the detection of a specific antibody response against Mycoplasma gallisepticum. Avian Pathol. 1993;22(3):481–94.

    Article  CAS  Google Scholar 

  9. Ostertag F, Weiss J, McClements DJ. Low-energy formation of edible nanoemulsions: factors influencing droplet size produced by emulsion phase inversion. J Colloid Interface Sci. 2012;388(1):95–102.

    Article  CAS  Google Scholar 

  10. Naskar MK, Kundu D, Chatterjee M. Effect of process parameters on surfactant-based synthesis of hydroxy sodalite particles. Mater Lett. 2011;65(3):436–8.

    Article  CAS  Google Scholar 

  11. The HLB System. https://caliscc.org/images/presentations/Mentor_2015_HLB.pdf Accessed 25 Sept 2018.

  12. Mair KH, Koinig H, Gerner W, Höhne A, Bretthauer J, Kroll JJ, et al. Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS® MLV. Vet Microbiol. 2015;176(3–4):352–7.

    Article  CAS  Google Scholar 

  13. Fox CB, Anderson RC, Dutill TS, Goto Y, Reed SG, Vedvick TS. Monitoring the effects of component structure and source on formulation stability and adjuvant activity of oil-in-water emulsions. Colloids Surf B: Biointerfaces. 2008;65(1):98–105.

    Article  CAS  Google Scholar 

  14. Nepal PR, Han HK, Choi HK. Preparation and in vitro–in vivo evaluation of Witepsol® H35 based self-nanoemulsifying drug delivery systems (SNEDDS) of coenzyme Q10. Eur J Pharm Sci. 2010;39(4):224–32.

    Article  CAS  Google Scholar 

  15. Honary S, Zahir F. Effect of zeta potential on the properties of nano-drug delivery systems-a review (part 2). Trop J Pharm Res. 2013;12(2):265–73.

    Google Scholar 

  16. Galliher-Beckley A, Pappan LK, Madera R, Burakova Y, Waters A, Nickles M, et al. Characterization of a novel oil-in-water emulsion adjuvant for swine influenza virus and Mycoplasma hyopneumoniae vaccines. Vaccine. 2015;33(25):2903–8.

    Article  CAS  Google Scholar 

  17. Fan Y, Ma X, Ma L, Zhang J, Zhang W, Song X. Antioxidative and immunological activities of ophiopogon polysaccharide liposome from the root of Ophiopogon japonicus. Carbohydr Polym. 2016;135:110–20.

    Article  CAS  Google Scholar 

  18. Burakova Y, Madera R, McVey S, Schlup JR, Shi J. Adjuvants for animal vaccines. Viral Immunol. 2018;31(1):11–22.

    Article  CAS  Google Scholar 

Download references

Funding

This research was supported by Technology Development Program (Project Nos. 316093-2 and 316094-2) for Bio-industry, Ministry for Agriculture, Food and Rural Affairs, Republic of Korea. This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1A6A1A03011325).

Author information

Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Rakesh Bastola, Jo-Eun Seo, Gyubin Noh, Taekwang Keum, Sooyeun Lee & Sangkil Lee

  2. Komipharm International Co., Ltd., 17 Gyeongje-ro, Siheung-si, Gyonggi-do, 15094, Republic of Korea

    Ju Hun Kim & Jong Il Shin

Authors
  1. Rakesh Bastola
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jo-Eun Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gyubin Noh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Taekwang Keum
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ju Hun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jong Il Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sooyeun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Sangkil Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sangkil Lee.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflicts of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bastola, R., Seo, JE., Noh, G. et al. Determination of Mycoplasma hyopneumoniae-Specific IgG, IgG1, and IgG2a Titers in BALB/c Mice Induced by Mineral Oil-Based Oil-in-Water Emulsion Adjuvants Prepared Using a Self-Emulsifying Drug Delivery System. AAPS PharmSciTech 20, 31 (2019). https://doi.org/10.1208/s12249-018-1245-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1208/s12249-018-1245-3

KEY WORDS

Search

Navigation