Abstract
The study aimed at investigating the dynamics of the distribution and localization of the gap junction protein connexin-43 (Cx43) in rat dorsal root ganglion (DRG) cells at different stages of postnatal ontogenesis to assess morphological signs of age-related changes in intercellular interactions. The work was performed on Wistar rats aged 4 and 18 months using immunohistochemical methods. Glial cells were detected using antibodies to glutamine synthetase, and Iba-1 marker was used for macrophages. It has been established that connexin-43-containing structures are identified predominantly in satellite glial cells of young and aging animals. Sensory neurons, as well as DRG macrophages, of the examined age groups do not contain connexin-43. When analyzing age-related changes in intercellular contacts in the DRG of rats, it was found that areas enriched with connexin-43, corresponding to plaques of protein channels that ensure metabolic interaction of satellite glial cells in the dorsal root ganglia, became more numerous with age. This fact may indicate activation of interaction between glial cells in the sensory ganglia during rat aging.
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The study was supported by the Russian Science Foundation (project no. 23-25-10003, https://rscf.ru/project/23-25-10003) and the St. Petersburg Science Foundation, Russia in accordance with agreement no. 23-25-10003 dated May 5, 2023.
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E.A. Kolos: developing a research plan, performing immunohistochemical reactions, analyzing the material, writing the text of the article; D.E. Korzhevsky: editing the text of the article.
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All studies were conducted in accordance with the principles of biomedical ethics as set out in the Declaration of Helsinki of 1964 and its subsequent amendments. They were also approved by the Ethics Committee of the Institute of Experimental Medicine (St. Petersburg, Russia), protocol no. 4/22 of September 29, 2022.
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Translated by I. Fridlyanskaya
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Abbreviations: PNS—peripheral nervous system; Cx43—connexin-43; DRG—dorsal root ganglion.
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Kolos, E.A., Korzhevsky, D.E. Gap Junction Protein Connexin-43 in a Rat Dorsal Root Ganglion. Cell Tiss. Biol. 18, 189–198 (2024). https://doi.org/10.1134/S1990519X23700049
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DOI: https://doi.org/10.1134/S1990519X23700049