Abstract
The antitumor properties of aqueous extracts of Inonotus rheades basidiomycete mushrooms were evaluated when the fungi were cultured on wood discs and wood shavings (birch) at 25 ± 1°C in the dark and under constant illumination with blue light (12.8 W/m2). The aqueous fraction containing water-soluble polysaccharides was isolated and analyzed: WS-5 fraction cultured on wood discs under blue light illumination, WS-8 fraction grown under similar conditions on wood shavings; WS-6 fraction grown in the dark on wood discs; and WS-7 fraction grown under similar conditions on wood shavings. The antitumor effects of the extracts were studied on the model of Ehrlich ascites carcinoma in mice. It was found that the extracts isolated from the basidial fungus Inonotus rheades exhibit antitumor properties and that their accumulation during growth is determined by different cultivation conditions.
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The study was supported by the Russian Foundation for Basic Research and the Government of the Irkutsk oblast (project no. 20-44-380010).
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Statement on the welfare of animals. The experiments were carried out according to the rules of humane treatment of animals and on the basis of the permission of the Ethics Committee of the Irkutsk Research Center for Surgery and Traumatology no. 04 dated April 23, 2021.
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Borovskii, G.B., Gornostai, T.G., Polyakova, M.S. et al. Antitumor Properties of Aqueous Extracts from Inonotus rheades Mycelium and Their Evaluation under Various Cultivation Conditions. Dokl Biochem Biophys 505, 151–155 (2022). https://doi.org/10.1134/S1607672922040019
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DOI: https://doi.org/10.1134/S1607672922040019