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Acyl-Modified Water-Soluble Chitosan Derivatives as Carriers for Adsorption Immobilization of Papain

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Abstract

Acid hydrolysis of hydrochloric acid in a 0.1-M aqueous solution has led to the formation of chitosan samples of different molecular weights (350 and 200 kDa), which have been used for the synthesis of N-succinyl chitosan and N-maleoyl chitosan. The structure of these target compounds has been confirmed by IR spectroscopy. The degrees of substitution have been calculated using the titrimetric data. Papain has been immobilized by adsorption on chitosan and its derivatives. The values of the optimal ratio of the protein content (mg/g of a carrier), total activity (units/mL of a solution), and specific activity (units/mg of the protein) were evaluated when immobilizing papain on chitosan and N-succinyl chitosan of 200 kDa.

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REFERENCES

  1. Ravishankar, K. and Dhamodharan, R., React. Funct. Polym., 2020, vol. 149, p. 104517. https://doi.org/10.1016/j.reactfunctpolym.2020.104517

    Article  CAS  Google Scholar 

  2. Qureshi, M.A., Nishat, N., Jadoun, S., and Ansari, M.Z., Carbohydr. Polym. Technol. Appl., 2020, vol. 1, art. ID 100014. https://doi.org/10.1016/j.carpta.2020.100014

    Article  Google Scholar 

  3. Sanchez-Salvador, J.L., Balea, A., Concepcion Monte, M., Negro, C., and Blanco, A., Int. J. Biol. Macromol., 2021, vol. 178, pp. 325–343. https://doi.org/10.1016/j.ijbiomac.2021.02.200

    Article  CAS  PubMed  Google Scholar 

  4. Rizwan, M., Gilani, S.R., Durani, A.I., and Naseem, S., J. Adv. Res., 2021, vol. 33, pp. 15–40. https://doi.org/10.1016/j.jare.2021.03.007

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Pellá, M.C.G., Lima-Tenório, M.K., Tenório-Neto, E.T., Guilherme, M.R., Muniz, E.C., and Rubira, A.F., Carbohydr. Res., 2018, vol. 196, pp. 233–245. https://doi.org/10.1016/j.carbpol.2018.05.033

    Article  CAS  Google Scholar 

  6. Peers, S., Montembault, A., and Ladavière, C., J. Controlled Release, 2020, vol. 326, pp. 150–163. https://doi.org/10.1016/j.jconrel.2020.06.012

    Article  CAS  Google Scholar 

  7. Qu, B. and Luo, Y., Int. J. Biol. Macromol., 2020, vol. 152, pp. 437–448. https://doi.org/10.1016/j.ijbiomac.2020.02.240

    Article  CAS  PubMed  Google Scholar 

  8. Pita-López, M.L., Fletes-Vargas, G., Espinosa-Andrews, H., and Rodríguez-Rodríguez, R., Eur. Polym. J., 2021, vol. 145, art. ID 110176. https://doi.org/10.1016/j.eurpolymj.2020.110176

    Article  CAS  Google Scholar 

  9. Jiang, T., James, R., Kumbar, S.G., and Laurencin, C.T., in Natural and Synthetic Biomedical Polymers, Kumbar, S.G., Laurencin, C.T., and Deng, M., Eds., Amsterdam: Elsevier Science, 2014, pp. 91–113. https://doi.org/10.1016/b978-0-12-396983-5.00005-3

  10. Akpan, E.I., Gbenebor, O.P., Adeosun, S.O., and Cletus, O., in Handbook of Chitin and Chitosan, Gopi, S., Thomas, S., and Pius, A., Eds., Amsterdam: Elsevier Science, 2020, pp. 131–164. https://doi.org/10.1016/b978-0-12-817970-3.00005-5

  11. Niu, X., Zhu, L., Xi, L., Guo, L., and Wang, H., Food Control., 2019, vol. 108, art. ID 106829. https://doi.org/10.1016/j.foodcont.2019.106829

    Article  CAS  Google Scholar 

  12. Holyavka, M.G., Evstigneev, M.P., Artyukhov, V.G., and Savin, V.V., J. Mol. Catal. B: Enzym., 2016, vol. 129, pp. 1–5. https://doi.org/10.1016/j.molcatb.2016.03.006

    Article  CAS  Google Scholar 

  13. Holyavka, M.G., Kayumov, A.R., Baydamshina, D.R., Koroleva, V.A., Trizna, E.Y., Trushin, M.V., and Artyukhov, V.G., Int. J. Biol. Macromol., 2018, vol. 115, pp. 829–834. https://doi.org/10.1016/j.ijbiomac.2018.04.107

    Article  CAS  PubMed  Google Scholar 

  14. Holyavka, M.G., Kondratyev, M.S., Lukin, A.N., Agapov, B.L., and Artyukhov, V.G., Int. J. Biol. Macromol., 2019, vol. 138, pp. 681–692. https://doi.org/10.1016/j.ijbiomac.2019.07.132

    Article  CAS  PubMed  Google Scholar 

  15. Holyavka, M., Faizullin, D., Koroleva, V., Olshannikova, S., Zakhartchenko, N., Zuev, Yu., Kondratyev, M., Zakharova, E., and Artyukhov, V., Int. J. Biol. Macromol., 2021, vol. 180, pp. 161–176. https://doi.org/10.1016/j.ijbiomac.2021.03.016

    Article  CAS  PubMed  Google Scholar 

  16. Jain, J., Int. J. Eng. Appl. Sci. Technol., 2020, vol. 5, pp. 193–197.

    Google Scholar 

  17. Vasconcelos, N.F., Cunha, A.P., Ricardo, N.M.P.S., Freire, R.S., de Araújo Pinto Vieira, L., Brígida, A.I.S., de Fátima Borges, M., de Freitas Rosa, M., Vieira, R.S., and Andrade, F.K., Int. J. Biol. Macromol., 2020, vol. 165, pp. 3065–3077. https://doi.org/10.1016/j.ijbiomac.2020.10.200

    Article  CAS  PubMed  Google Scholar 

  18. Kuznetsov, V.A., Lavlinskaya, M.S., Ostankova, I.V., Shatalov, G.V., Shikhaliev, K.S., and Ryzhkova, E.A., Polym. Bull., 2018, vol. 75, pp. 1237–1251. https://doi.org/10.1007/s00289-017-2091-2

    Article  CAS  Google Scholar 

  19. Novikov, V.Y., Russ. J. Appl. Chem., 2004, vol. 77, pp. 484–487. https://doi.org/10.1023/b:rjac.0000031297.24742.b9

    Article  CAS  Google Scholar 

  20. Kasaai, M.R., Arul, J., and Charlet, G., Sci. World J., 2013, vol. 2013, art. ID 508540. https://doi.org/10.1155/2013/508540

    Article  Google Scholar 

  21. Santoso, J., Adiputra, K.C., Soerdirga, L.C., and Tarman, K., IOP Conf. Ser.: Earth Environ. Sci., 2020, vol. 414, art. ID 012021. https://doi.org/10.1088/1755-1315/414/1/012021

  22. Dimzon, I.K.D. and Knepper, T.P., Int. J. Biol. Macromol., 2015, vol. 72, pp. 939–945. https://doi.org/10.1016/j.ijbiomac.2014.09.050

    Article  CAS  PubMed  Google Scholar 

  23. Mucha, M. and Pawlak, A., Polimery, 2002, vol. 47, pp. 509–516. https://doi.org/10.14314/polimery.2002.509

    Article  CAS  Google Scholar 

  24. Aiping, Z., Tian, C., Lanhua, Y., Hao, W., and Ping, L., Carbohydr. Polym., 2006, vol. 66, pp. P. 274–279. https://doi.org/10.1016/j.carbpol.2006.03.014

  25. Sorokin, A. and Lavlinskaya, M., Polym. Bull., 2021. https://doi.org/10.1007/s00289-020-03521-9

  26. Kasaai, M.R., Carbohydr. Polym., 2007, vol. 68, pp. 477–488. https://doi.org/10.1016/j.carbpol.2006.11.006

    Article  CAS  Google Scholar 

  27. Rosebrough, N.J., Faar, A.L., and Randall, R.J., J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    Article  Google Scholar 

  28. Sabirova, A.R., Rudakova, N.L., Balaban, N.P., Ilyinskaya, O.N., Demidyuk, I.V., Kostrov, S.V., Rudenskaya, G.N., and Sharipova, M.R., FEBS Lett., 2010, vol. 584, pp. 4419–4425. https://doi.org/10.1016/j.febslet.2010.09.049

    Article  CAS  PubMed  Google Scholar 

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ACKNOWLEDGMENTS

Experiments were performed using the scientific and technical base of the Core Research Center of Voronezh State University.

Funding

The work was supported by the Russian Science Foundation (project no. 21-74-20053).

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Authors and Affiliations

  1. Voronezh State University, 1394018, Voronezh, Russia

    A. V. Sorokin, S. S. Olshannikova, N. V. Malykhina, F. A. Sakibaev, M. G. Holyavka, M. S. Lavlinskaya & V. G. Artyukhov

  2. Voronezh State University of Engineering Technologies, 394036, Voronezh, Russia

    A. V. Sorokin & M. S. Lavlinskaya

  3. Sevastopol State University, 299053, Sevastopol, Russia

    M. G. Holyavka

Authors
  1. A. V. Sorokin
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  2. S. S. Olshannikova
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  3. N. V. Malykhina
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  4. F. A. Sakibaev
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  5. M. G. Holyavka
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  6. M. S. Lavlinskaya
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  7. V. G. Artyukhov
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Corresponding author

Correspondence to M. G. Holyavka.

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This article does not contain any studies with the use of humans or animals as objects of research.

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Translated by A. Levina

Corresponding author: phone: +7 (473) 220-85-86.

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Sorokin, A.V., Olshannikova, S.S., Malykhina, N.V. et al. Acyl-Modified Water-Soluble Chitosan Derivatives as Carriers for Adsorption Immobilization of Papain. Russ J Bioorg Chem 48, 310–320 (2022). https://doi.org/10.1134/S1068162022020212

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