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Distinctive features of immune proteasome expression during the development of the central nervous system in rats

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Abstract

Formation of the central nervous system in ontogeny and its functioning in adult mammals are controlled by the universal ubiquitin-proteasome proteolytic system. The aim of the present work was to study the dynamics of immune proteasome expression as compared with the dynamics of chymotrypsin-like and caspase-like activities of the proteasome (ChLA and CLA) and expression of the transcription factor Zif268 in selected brain structures (cortex, hippocampus, and brainstem) in embryonic (days E19 and E21 of embryonic development) and early postnatal (postnatal days P1, P3, P4, P5, P7, and P15) development in rats. ChLA and CLA were determined in clarified homogenates of rat brain structures using commercial fluorogenic oligopeptide substrates Suc-LLVY-AMC and Z-LLG-AMC, respectively. Levels of constitutive (β1 and β5) and immune (LMP7 and LMP2) proteasome subunits, as well as those of proteasome activators PA700 and PA28 and the transcription factor Zif268, were evaluated by Western blotting. Increased expression of the immune subunit LMP7 in the cerebral cortex and hippocampus was observed during the period of active formation of the biochemical mediator structure of neurons (P7-P15). Increased ChLA and CLA were registered in the cortex during this period. The content of the immune subunit LMP2 was significantly elevated in all brain structures between P7 and P15. The content of the constitutive proteolytic subunit β1 in all structures was lower on P4 than on P1 and increased to the same level as on P1 by P15. However, the expression level of the constitutive proteolytic β5 subunit of the proteasome in the cortex, hippocampus, and brainstem increased starting from E21 and reached maximal values at P3, P5 and P1, respectively, with a sharp decline in all structures by P7. Expression of the immune subunit LMP2 and the constitutive subunit β1 in all structures increased sharply around P15 concomitantly to the increase in the content of the immune subunit LMP7. Moreover, a positive correlation of increased expression of the activator PA28 and the constitutive β5 subunit in the hippocampus during P3-P5 and in the brain stem during P1-P5 has been demonstrated. Elevation of proteasome CLA and expression levels of immune proteasome subunits LMP2 and LMP7 during days P7-P15 of postnatal development in the brain regions investigated were related to the expression of the transcription factor Zif268. Immune proteasomes probably play an important role in the regulation of key biochemical processes during early ontogenesis of the central nervous system and are necessary for the emergence and maintenance of synaptic plasticity in rat brain structures analyzed.

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Abbreviations

AMC:

7-amido-4-methylcoumarin

Suc:

succinyl

Z:

benzyloxycarbonyl

P1-P15:

days of postnatal development

UPS:

the ubiquitin-proteasome system

CNS:

central nervous system

E19-E21:

days of embryonic development

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Authors and Affiliations

  1. Kol’tsov Institute of Developmental Biology of the Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119334, Russia

    A. Sh. Orlova, Yu. V. Lyupina, S. B. Abaturova & N. P. Sharova

Authors
  1. A. Sh. Orlova
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  2. Yu. V. Lyupina
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  3. S. B. Abaturova
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  4. N. P. Sharova
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Correspondence to Yu. V. Lyupina.

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Original Russian Text © A.Sh. Orlova, Yu.V. Lyupina, S.B. Abaturova, N.P. Sharova, 2014, published in Bioorganicheskaya Khimiya, 2014, Vol. 40, No. 6, pp. 703–711.

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Orlova, A.S., Lyupina, Y.V., Abaturova, S.B. et al. Distinctive features of immune proteasome expression during the development of the central nervous system in rats. Russ J Bioorg Chem 40, 649–657 (2014). https://doi.org/10.1134/S1068162014060119

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  • DOI: https://doi.org/10.1134/S1068162014060119

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